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Real-time imaging through optical fiber array-assisted laser-induced fluorescence of capillary electrophoretic enantiomer separations

Johansson, T; Petersson, M; Johansson, Jonas LU and Nilsson, S (1999) In Analytical Chemistry 71(19). p.4190-4197
Abstract
An advanced detection system based on laser-induced fluorescence imaging for capillary electrophoresis (CE) is presented. An optical fiber array was constructed for collection and transportation of the emitted fluorescent light to the charge-coupled device (CCD) camera. The fiber array makes the setup compact compared with a setup where the capillary is imaged through a camera objective. The imaging detector captures the sample zones in motion during the migration through the capillary. This allows unique studies on dynamic events otherwise unrevealed. During the study, unexplained nonlinear migration behavior was revealed. Enantiomer separations of dansylated amino acids using cyclodextrins, imaged between 1.5 and 12 cm of a 28-cm-long... (More)
An advanced detection system based on laser-induced fluorescence imaging for capillary electrophoresis (CE) is presented. An optical fiber array was constructed for collection and transportation of the emitted fluorescent light to the charge-coupled device (CCD) camera. The fiber array makes the setup compact compared with a setup where the capillary is imaged through a camera objective. The imaging detector captures the sample zones in motion during the migration through the capillary. This allows unique studies on dynamic events otherwise unrevealed. During the study, unexplained nonlinear migration behavior was revealed. Enantiomer separations of dansylated amino acids using cyclodextrins, imaged between 1.5 and 12 cm of a 28-cm-long 50-mu m i.d. capillary, were used for evaluation of the system. Comparing the optical fiber array with a camera lens system, the signal-to-noise-ratio (S/N) was 10 times higher. This is due to a combination of both higher signal and lower noise levels. To improve the S/N ratio further, a computer program for signal processing was designed. Using dichlorofluorescein, a concentration limit of detection (CLOD) of 350 pM was achieved and improved 10 times to 35 pM with computer postprocessing using 79 images. This is equal to 400 zeptomole for a 3-mm-long sample zone in a 50-mu m i.d. capillary. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
71
issue
19
pages
4190 - 4197
publisher
The American Chemical Society
external identifiers
  • scopus:0033215414
ISSN
1520-6882
DOI
10.1021/ac990398l
language
English
LU publication?
yes
id
d0b19dce-8833-4b47-b878-ad24220e1757 (old id 2258513)
date added to LUP
2012-02-20 21:00:29
date last changed
2017-01-01 04:25:04
@article{d0b19dce-8833-4b47-b878-ad24220e1757,
  abstract     = {An advanced detection system based on laser-induced fluorescence imaging for capillary electrophoresis (CE) is presented. An optical fiber array was constructed for collection and transportation of the emitted fluorescent light to the charge-coupled device (CCD) camera. The fiber array makes the setup compact compared with a setup where the capillary is imaged through a camera objective. The imaging detector captures the sample zones in motion during the migration through the capillary. This allows unique studies on dynamic events otherwise unrevealed. During the study, unexplained nonlinear migration behavior was revealed. Enantiomer separations of dansylated amino acids using cyclodextrins, imaged between 1.5 and 12 cm of a 28-cm-long 50-mu m i.d. capillary, were used for evaluation of the system. Comparing the optical fiber array with a camera lens system, the signal-to-noise-ratio (S/N) was 10 times higher. This is due to a combination of both higher signal and lower noise levels. To improve the S/N ratio further, a computer program for signal processing was designed. Using dichlorofluorescein, a concentration limit of detection (CLOD) of 350 pM was achieved and improved 10 times to 35 pM with computer postprocessing using 79 images. This is equal to 400 zeptomole for a 3-mm-long sample zone in a 50-mu m i.d. capillary.},
  author       = {Johansson, T and Petersson, M and Johansson, Jonas and Nilsson, S},
  issn         = {1520-6882},
  language     = {eng},
  number       = {19},
  pages        = {4190--4197},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Real-time imaging through optical fiber array-assisted laser-induced fluorescence of capillary electrophoretic enantiomer separations},
  url          = {http://dx.doi.org/10.1021/ac990398l},
  volume       = {71},
  year         = {1999},
}