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Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson, H; Johansson, Jonas LU ; Svanberg, Katarina LU ; Svanberg, Sune LU ; Jori, G; Reddi, E; Segalla, A; Gust, D and Moore, T. A (1997) In British Journal of Cancer 76(3). p.355-364
Abstract
The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)(3)) and trimethoxylated (CP(OMe)(3)) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the... (More)
The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)(3)) and trimethoxylated (CP(OMe)(3)) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
British Journal of Cancer
volume
76
issue
3
pages
355 - 364
publisher
Nature Publishing Group
external identifiers
  • scopus:12644304244
ISSN
1532-1827
DOI
10.1038/bjc.1997.390
language
English
LU publication?
yes
id
cb37a8f4-2f23-4004-9bde-153287fb4396 (old id 2259060)
date added to LUP
2012-02-17 22:48:25
date last changed
2017-01-01 07:32:44
@article{cb37a8f4-2f23-4004-9bde-153287fb4396,
  abstract     = {The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)(3)) and trimethoxylated (CP(OMe)(3)) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.},
  author       = {Nilsson, H and Johansson, Jonas and Svanberg, Katarina and Svanberg, Sune and Jori, G and Reddi, E and Segalla, A and Gust, D and Moore, T. A},
  issn         = {1532-1827},
  language     = {eng},
  number       = {3},
  pages        = {355--364},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice},
  url          = {http://dx.doi.org/10.1038/bjc.1997.390},
  volume       = {76},
  year         = {1997},
}