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Optical absorption of blood depends on temperature during a 0.5 ms laser pulse at 586 nm

Verkruysse, W; Nilsson, A. M. K; Milner, T. E; Beek, J. F; Lucassen, G. W and van Gemert, M. J. C (1998) In Photochemistry and Photobiology 67(3). p.276-281
Abstract
Optical properties are important parameters in port wine stain laser treatment models. In this study we investigated whether changes in blood optical properties occur during a 0.5 ms laser pulse, Blood from three volunteers was irradiated in vitro with laser pulses (radiant exposure 2-12 J cm(-2), wavelength 586 nm, pulse length 0.5 ms). Reflection and transmission coefficients, measured using double integrating spheres, decreased slightly during the first part of the pulse. At 2.9 J cm(-2) radiant exposure, the reflectance increased, independent of total radiant exposure of the pulse, This was-caused by blood coagulation, A second sudden increase in reflection and a significant increase in transmission occurred near 6.3 9 cm(-2) and was... (More)
Optical properties are important parameters in port wine stain laser treatment models. In this study we investigated whether changes in blood optical properties occur during a 0.5 ms laser pulse, Blood from three volunteers was irradiated in vitro with laser pulses (radiant exposure 2-12 J cm(-2), wavelength 586 nm, pulse length 0.5 ms). Reflection and transmission coefficients, measured using double integrating spheres, decreased slightly during the first part of the pulse. At 2.9 J cm(-2) radiant exposure, the reflectance increased, independent of total radiant exposure of the pulse, This was-caused by blood coagulation, A second sudden increase in reflection and a significant increase in transmission occurred near 6.3 9 cm(-2) and was accompanied by a "popping" sound, indicating rapid expansion of bubbles due to blood vaporization. A multilayered model of blood was used to fit calculated transmission coefficient curves to the measurements and determine temperature-dependent optical blood absorption, Heat diffusion was shown to be of minor importance, A 2.5-fold increase in absorption for temperatures increasing from 20 to 100 degrees C, accurately describes transmission coefficients measured up to 2.9 J cm(-2). (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Photochemistry and Photobiology
volume
67
issue
3
pages
276 - 281
publisher
American Society for Photobiology
external identifiers
  • scopus:0032011135
ISSN
0031-8655
DOI
10.1111/j.1751-1097.1998.tb05199.x
language
English
LU publication?
no
id
82b494a8-af67-40bf-9014-3e4d7b4ca2ab (old id 2259653)
date added to LUP
2012-02-07 00:28:10
date last changed
2017-04-09 04:28:21
@article{82b494a8-af67-40bf-9014-3e4d7b4ca2ab,
  abstract     = {Optical properties are important parameters in port wine stain laser treatment models. In this study we investigated whether changes in blood optical properties occur during a 0.5 ms laser pulse, Blood from three volunteers was irradiated in vitro with laser pulses (radiant exposure 2-12 J cm(-2), wavelength 586 nm, pulse length 0.5 ms). Reflection and transmission coefficients, measured using double integrating spheres, decreased slightly during the first part of the pulse. At 2.9 J cm(-2) radiant exposure, the reflectance increased, independent of total radiant exposure of the pulse, This was-caused by blood coagulation, A second sudden increase in reflection and a significant increase in transmission occurred near 6.3 9 cm(-2) and was accompanied by a "popping" sound, indicating rapid expansion of bubbles due to blood vaporization. A multilayered model of blood was used to fit calculated transmission coefficient curves to the measurements and determine temperature-dependent optical blood absorption, Heat diffusion was shown to be of minor importance, A 2.5-fold increase in absorption for temperatures increasing from 20 to 100 degrees C, accurately describes transmission coefficients measured up to 2.9 J cm(-2).},
  author       = {Verkruysse, W and Nilsson, A. M. K and Milner, T. E and Beek, J. F and Lucassen, G. W and van Gemert, M. J. C},
  issn         = {0031-8655},
  language     = {eng},
  number       = {3},
  pages        = {276--281},
  publisher    = {American Society for Photobiology},
  series       = {Photochemistry and Photobiology},
  title        = {Optical absorption of blood depends on temperature during a 0.5 ms laser pulse at 586 nm},
  url          = {http://dx.doi.org/10.1111/j.1751-1097.1998.tb05199.x},
  volume       = {67},
  year         = {1998},
}