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Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours

Stenberg, M ; Soto Thompson, Marcelo LU ; Johansson, Thomas LU ; Pålsson, Sara LU ; af Klinteberg, C ; Andersson-Engels, Stefan LU ; Stenram, U ; Svanberg, Sune LU and Svanberg, Katarina LU (2000) Conference on Optical Biopsy and Tissue Optics, 2000 4161(32). p.151-157
Abstract
A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry... (More)
A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
OPTICAL BIOPSY AND TISSUE OPTICS
editor
Bigio, IJ ; Muller, GJ ; Puppels, GJ ; Steiner, RW and Svanberg, Katarina
volume
4161
issue
32
pages
151 - 157
publisher
SPIE
conference name
Conference on Optical Biopsy and Tissue Optics, 2000
conference location
Amsterdam, Netherlands
conference dates
2000-07-05 - 2000-07-06
external identifiers
  • scopus:0034587109
ISSN
0277-786X
1996-756X
ISBN
0-8194-3817-0
DOI
10.1117/12.409325
language
English
LU publication?
yes
id
f69a643e-0ae7-435e-9c8e-225d8dadc5f8 (old id 2260943)
date added to LUP
2016-04-01 11:53:21
date last changed
2025-01-14 22:30:39
@inproceedings{f69a643e-0ae7-435e-9c8e-225d8dadc5f8,
  abstract     = {{A recently developed multiple fibre system for treating malignant tumours with interstitial photodynamic therapy was used in studies on rats with colon adenocarcinoma inoculated into the muscles of the hind legs. The animals were intraperitonially administrated delta -aminolevulinic acid (ALA), which is metabolised to protoporphyrin IX (PpIX) in the tissue. The treatment system consists of a laser light source, a beam-splitting system dividing the light into three or six output fibres and a dosimetry programme calculating the optimal fibre position within the tumour as well as the treatment time needed to obtain a given threshold value of the light dose. One aim of the study was to compare the treatment outcome with the modelled dosimetry predictions. Tumour reduction was examined three days post treatment. A volume decrease was found in 85\% of the treated tumours. The mean volume reduction was 44\%, with one tumour completely disappearing. Histopathological examination three days post treatment showed substantial necrotic parts which, however, to a smaller extent were present also for non-treated tumours. These results indicated that the tumours have been under treated and the light dose has to be increased. Measurements of the build-up and photo-induced bleaching of PpIX using laser-induced fluorescence were also performed during the experiments.}},
  author       = {{Stenberg, M and Soto Thompson, Marcelo and Johansson, Thomas and Pålsson, Sara and af Klinteberg, C and Andersson-Engels, Stefan and Stenram, U and Svanberg, Sune and Svanberg, Katarina}},
  booktitle    = {{OPTICAL BIOPSY AND TISSUE OPTICS}},
  editor       = {{Bigio, IJ and Muller, GJ and Puppels, GJ and Steiner, RW and Svanberg, Katarina}},
  isbn         = {{0-8194-3817-0}},
  issn         = {{0277-786X}},
  language     = {{eng}},
  number       = {{32}},
  pages        = {{151--157}},
  publisher    = {{SPIE}},
  title        = {{Interstitial photodynamic therapy - diagnostic measurements and treatment in rat malignant experimental tumours}},
  url          = {{https://lup.lub.lu.se/search/files/2688386/2297593.pdf}},
  doi          = {{10.1117/12.409325}},
  volume       = {{4161}},
  year         = {{2000}},
}