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Solution structure of human prolactin

Teilum, Kaare LU ; Hoch, JC; Goffin, V; Kinet, S; Martial, JA and Kragelund, BB (2005) In Journal of Molecular Biology 351(4). p.810-823
Abstract
We report the solution structure of human prolactin determined by NMR spectroscopy. Our result is a significant improvement over a previous structure in terms of number and distribution of distance restraints, regularity of secondary structure, and potential energy. More significantly, the structure is sufficiently different that it leads to different conclusions regarding the mechanism of receptor activation and initiation of signal transduction. Here, we compare the structure of unbound prolactin to structures of both the homologue ovine placental lacto en and growth hormone. The structures of unbound and receptor bound prolactin/placental lactogen are similar and no noteworthy structural changes occur upon receptor binding. The... (More)
We report the solution structure of human prolactin determined by NMR spectroscopy. Our result is a significant improvement over a previous structure in terms of number and distribution of distance restraints, regularity of secondary structure, and potential energy. More significantly, the structure is sufficiently different that it leads to different conclusions regarding the mechanism of receptor activation and initiation of signal transduction. Here, we compare the structure of unbound prolactin to structures of both the homologue ovine placental lacto en and growth hormone. The structures of unbound and receptor bound prolactin/placental lactogen are similar and no noteworthy structural changes occur upon receptor binding. The observation of enhanced binding at the second receptor site when the first site is occupied has been widely interpreted to indicate conformational change induced by binding the first receptor. However, our results indicate that this enhanced binding at the second site could be due to receptor-receptor interactions or some other free energy sources rather than conformational change in the hormone. Titration of human prolactin with the extracellular domain of the human prolactin receptor was followed by NMR, gel filtration and electrophoresis. Both binary and ternary hormone-receptor complexes are clearly detectable by gel filtration and electrophoresis. The binary complex is not observable by NMR, possibly due to a dynamic equilibrium in intermediate exchange within the complex. The ternary complex of one hormone molecule bound to two receptor molecules is on the contrary readily detectable by NMR. This is in stark contrast to the widely held view that the ternary prolactin-receptor complex is only transiently formed. Thus, our results lead to improved understanding of the prolactin-prolactin receptor interaction. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
four-helix bundle, receptor interactions, NMR, cytokine, hormone
in
Journal of Molecular Biology
volume
351
issue
4
pages
810 - 823
publisher
Elsevier
external identifiers
  • wos:000231320800010
  • pmid:16045928
  • scopus:23444431656
ISSN
1089-8638
DOI
10.1016/j.jmb.2005.06.042
language
English
LU publication?
no
id
0ced3add-ae94-4fb6-bb43-d948df616294 (old id 226710)
date added to LUP
2007-10-05 16:02:36
date last changed
2017-10-01 04:50:53
@article{0ced3add-ae94-4fb6-bb43-d948df616294,
  abstract     = {We report the solution structure of human prolactin determined by NMR spectroscopy. Our result is a significant improvement over a previous structure in terms of number and distribution of distance restraints, regularity of secondary structure, and potential energy. More significantly, the structure is sufficiently different that it leads to different conclusions regarding the mechanism of receptor activation and initiation of signal transduction. Here, we compare the structure of unbound prolactin to structures of both the homologue ovine placental lacto en and growth hormone. The structures of unbound and receptor bound prolactin/placental lactogen are similar and no noteworthy structural changes occur upon receptor binding. The observation of enhanced binding at the second receptor site when the first site is occupied has been widely interpreted to indicate conformational change induced by binding the first receptor. However, our results indicate that this enhanced binding at the second site could be due to receptor-receptor interactions or some other free energy sources rather than conformational change in the hormone. Titration of human prolactin with the extracellular domain of the human prolactin receptor was followed by NMR, gel filtration and electrophoresis. Both binary and ternary hormone-receptor complexes are clearly detectable by gel filtration and electrophoresis. The binary complex is not observable by NMR, possibly due to a dynamic equilibrium in intermediate exchange within the complex. The ternary complex of one hormone molecule bound to two receptor molecules is on the contrary readily detectable by NMR. This is in stark contrast to the widely held view that the ternary prolactin-receptor complex is only transiently formed. Thus, our results lead to improved understanding of the prolactin-prolactin receptor interaction.},
  author       = {Teilum, Kaare and Hoch, JC and Goffin, V and Kinet, S and Martial, JA and Kragelund, BB},
  issn         = {1089-8638},
  keyword      = {four-helix bundle,receptor interactions,NMR,cytokine,hormone},
  language     = {eng},
  number       = {4},
  pages        = {810--823},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {Solution structure of human prolactin},
  url          = {http://dx.doi.org/10.1016/j.jmb.2005.06.042},
  volume       = {351},
  year         = {2005},
}