Advanced

Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5

Lopes, S M C D; van den Driesche, S; Carvalho, R L C; Larsson, Jonas LU ; Eggen, B; Surani, M A and Mummery, C L (2005) In Developmental Biology 284(1). p.194-203
Abstract
Transforming growth factor beta (TGF beta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGF beta signaling via the type I receptor ALK5. TGF beta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E) 10. Unexpectedly, the absence of TGF beta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere... (More)
Transforming growth factor beta (TGF beta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGF beta signaling via the type I receptor ALK5. TGF beta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E) 10. Unexpectedly, the absence of TGF beta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGF beta signaling in migration of PGCs in vivo distinct from that described previously. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
migration, TGF beta, primordial germ cells, mouse embryo, collagen, hindgut
in
Developmental Biology
volume
284
issue
1
pages
194 - 203
publisher
Elsevier
external identifiers
  • wos:000231352800016
  • pmid:15993878
  • scopus:23244448133
ISSN
1095-564X
DOI
10.1016/j.ydbio.2005.05.019
language
English
LU publication?
yes
id
75206c1a-dc88-4391-8b6e-5b025e9b473e (old id 226959)
date added to LUP
2007-08-03 13:53:39
date last changed
2017-04-09 03:36:20
@article{75206c1a-dc88-4391-8b6e-5b025e9b473e,
  abstract     = {Transforming growth factor beta (TGF beta) inhibits proliferation and promotes the migration of primordial germ cells (PGCs) towards explants of gonadal ridges in vitro. However, its effects in vivo are still unclear. Here, we analyzed the behavior of PGCs in embryos lacking TGF beta signaling via the type I receptor ALK5. TGF beta in vivo was neither a chemoattractant for PGCs, nor did it affect their proliferation during migration towards the gonadal ridges up to embryonic day (E) 10. Unexpectedly, the absence of TGF beta signaling in fact resulted in significant facilitation of PGC migration out of the hindgut, due to the reduced deposition of collagen type I surrounding the gut of Alk5-deficient mutant embryos. Migratory PGCs adhere strongly to collagen; therefore, reduced collagen type I along the gut may result in reduced adhesion, facilitating migration into the dorsal mesenterium and gonadal ridges. Our results provide new evidence for the role of TGF beta signaling in migration of PGCs in vivo distinct from that described previously.},
  author       = {Lopes, S M C D and van den Driesche, S and Carvalho, R L C and Larsson, Jonas and Eggen, B and Surani, M A and Mummery, C L},
  issn         = {1095-564X},
  keyword      = {migration,TGF beta,primordial germ cells,mouse embryo,collagen,hindgut},
  language     = {eng},
  number       = {1},
  pages        = {194--203},
  publisher    = {Elsevier},
  series       = {Developmental Biology},
  title        = {Altered primordial germ cell migration in the absence of transforming growth factor beta signaling via ALK5},
  url          = {http://dx.doi.org/10.1016/j.ydbio.2005.05.019},
  volume       = {284},
  year         = {2005},
}