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Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.

Semmrich, Monika LU ; Plantinga, M; Svensson Frej, Marcus LU ; Uronen-Hansson, Heli LU ; Gustafsson, T; Mowat, Allan LU ; Yrlid, U; Lambrecht, B N and Agace, William LU (2012) In Mucosal Immunology 5(2). p.150-160
Abstract
The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA... (More)
The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mucosal Immunology
volume
5
issue
2
pages
150 - 160
publisher
Nature Publishing Group
external identifiers
  • wos:000300508700005
  • pmid:22166938
  • scopus:84857220793
ISSN
1933-0219
DOI
10.1038/mi.2011.61
language
English
LU publication?
yes
id
747715f7-9187-415f-a592-cfc149fccd4d (old id 2274086)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22166938?dopt=Abstract
date added to LUP
2012-01-03 19:23:20
date last changed
2017-11-15 11:55:50
@article{747715f7-9187-415f-a592-cfc149fccd4d,
  abstract     = {The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61.},
  author       = {Semmrich, Monika and Plantinga, M and Svensson Frej, Marcus and Uronen-Hansson, Heli and Gustafsson, T and Mowat, Allan and Yrlid, U and Lambrecht, B N and Agace, William},
  issn         = {1933-0219},
  language     = {eng},
  number       = {2},
  pages        = {150--160},
  publisher    = {Nature Publishing Group},
  series       = {Mucosal Immunology},
  title        = {Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.},
  url          = {http://dx.doi.org/10.1038/mi.2011.61},
  volume       = {5},
  year         = {2012},
}