Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.
(2012) In Mucosal Immunology 5(2). p.150-160- Abstract
- The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA... (More)
- The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2274086
- author
- Semmrich, Monika LU ; Plantinga, M ; Svensson Frej, Marcus LU ; Uronen-Hansson, Heli LU ; Gustafsson, T ; Mowat, Allan LU ; Yrlid, U ; Lambrecht, B N and Agace, William LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Mucosal Immunology
- volume
- 5
- issue
- 2
- pages
- 150 - 160
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000300508700005
- pmid:22166938
- scopus:84857220793
- pmid:22166938
- ISSN
- 1933-0219
- DOI
- 10.1038/mi.2011.61
- language
- English
- LU publication?
- yes
- id
- 747715f7-9187-415f-a592-cfc149fccd4d (old id 2274086)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22166938?dopt=Abstract
- date added to LUP
- 2016-04-04 07:53:25
- date last changed
- 2022-03-23 01:42:29
@article{747715f7-9187-415f-a592-cfc149fccd4d, abstract = {{The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61.}}, author = {{Semmrich, Monika and Plantinga, M and Svensson Frej, Marcus and Uronen-Hansson, Heli and Gustafsson, T and Mowat, Allan and Yrlid, U and Lambrecht, B N and Agace, William}}, issn = {{1933-0219}}, language = {{eng}}, number = {{2}}, pages = {{150--160}}, publisher = {{Nature Publishing Group}}, series = {{Mucosal Immunology}}, title = {{Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.}}, url = {{http://dx.doi.org/10.1038/mi.2011.61}}, doi = {{10.1038/mi.2011.61}}, volume = {{5}}, year = {{2012}}, }