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Remodeling of fracture callus in mice is consistent with mechanical loading and bone remodeling theory

Isaksson, Hanna LU ; Gröngröft, Ina; Wilson, Wouter; van Donkelaar, Corrinus C; van Rietbergen, Bert; Tami, Andrea; Huiskes, Rik and Ito, Keita (2009) In Journal of Orthopaedic Research 27(5). p.664-672
Abstract
During the remodeling phase of fracture healing in mice, the callus gradually transforms into a double cortex, which thereafter merges into one cortex. In large animals, a double cortex normally does not form. We investigated whether these patterns of remodeling of the fracture callus in mice can be explained by mechanical loading. Morphologies of fractures after 21, 28, and 42 days of healing were determined from an in vivo mid-diaphyseal femoral osteotomy healing experiment in mice. Bone density distributions from microCT at 21 days were converted into adaptive finite element models. To assess the effect of loading mode on bone remodeling, a well-established remodeling algorithm was used to examine the effect of axial force or bending... (More)
During the remodeling phase of fracture healing in mice, the callus gradually transforms into a double cortex, which thereafter merges into one cortex. In large animals, a double cortex normally does not form. We investigated whether these patterns of remodeling of the fracture callus in mice can be explained by mechanical loading. Morphologies of fractures after 21, 28, and 42 days of healing were determined from an in vivo mid-diaphyseal femoral osteotomy healing experiment in mice. Bone density distributions from microCT at 21 days were converted into adaptive finite element models. To assess the effect of loading mode on bone remodeling, a well-established remodeling algorithm was used to examine the effect of axial force or bending moment on bone structure. All simulations predicted that under axial loading, the callus remodeled to form a single cortex. When a bending moment was applied, dual concentric cortices developed in all simulations, corresponding well to the progression of remodeling observed experimentally and resulting in quantitatively comparable callus areas of woven and lamellar bone. Effects of biological differences between species or other reasons cannot be excluded, but this study demonstrates how a difference in loading mode could explain the differences between the remodeling phase in small rodents and larger mammals. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
27
issue
5
pages
664 - 672
publisher
John Wiley & Sons
external identifiers
  • scopus:65249165926
ISSN
1554-527X
language
English
LU publication?
no
id
1d51516b-17c0-4c8b-9027-b50def3af1e7 (old id 2275375)
date added to LUP
2012-01-27 10:19:45
date last changed
2017-11-12 03:26:08
@article{1d51516b-17c0-4c8b-9027-b50def3af1e7,
  abstract     = {During the remodeling phase of fracture healing in mice, the callus gradually transforms into a double cortex, which thereafter merges into one cortex. In large animals, a double cortex normally does not form. We investigated whether these patterns of remodeling of the fracture callus in mice can be explained by mechanical loading. Morphologies of fractures after 21, 28, and 42 days of healing were determined from an in vivo mid-diaphyseal femoral osteotomy healing experiment in mice. Bone density distributions from microCT at 21 days were converted into adaptive finite element models. To assess the effect of loading mode on bone remodeling, a well-established remodeling algorithm was used to examine the effect of axial force or bending moment on bone structure. All simulations predicted that under axial loading, the callus remodeled to form a single cortex. When a bending moment was applied, dual concentric cortices developed in all simulations, corresponding well to the progression of remodeling observed experimentally and resulting in quantitatively comparable callus areas of woven and lamellar bone. Effects of biological differences between species or other reasons cannot be excluded, but this study demonstrates how a difference in loading mode could explain the differences between the remodeling phase in small rodents and larger mammals.},
  author       = {Isaksson, Hanna and Gröngröft, Ina and Wilson, Wouter and van Donkelaar, Corrinus C and van Rietbergen, Bert and Tami, Andrea and Huiskes, Rik and Ito, Keita},
  issn         = {1554-527X},
  language     = {eng},
  number       = {5},
  pages        = {664--672},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Orthopaedic Research},
  title        = {Remodeling of fracture callus in mice is consistent with mechanical loading and bone remodeling theory},
  volume       = {27},
  year         = {2009},
}