Timing of collagen and mineral deposition in cortical bone and effect on tissue properties
Isaksson, Hanna LU
; Harjula, Terhi
; Koistinen, Arto
; Iivarinen, Jaakko
; Seppänen, Kari
; Arikoski, Jari
; Brama, Pieter
; Jurvelin, Jukka
and Helminen, Heikki
(2010)
In Journal of Orthopaedic Research
28(12).
p.1626-1633
- Abstract
- Abstract in Undetermined
We characterized the composition and mechanical properties of cortical bone during maturation and growth and in adult life in the rabbit. We hypothesized that the collagen network develops earlier than the mineralized matrix. Growth was monitored, and the rabbits were euthanized at birth (newborn), and at 1, 3, 6, 9, and 18 months of age. The collagen network was assessed biochemically (collagen content, enzymatic and non-enzymatic cross-links) in specimens from the mid-diaphysis of the tibia and femur and biomechanically (tensile testing) from decalcified whole tibia specimens. The mineralized matrix was analyzed using pQCT and 3-point bend tests from intact femur specimens. The collagen content and the... (More) - Abstract in Undetermined
We characterized the composition and mechanical properties of cortical bone during maturation and growth and in adult life in the rabbit. We hypothesized that the collagen network develops earlier than the mineralized matrix. Growth was monitored, and the rabbits were euthanized at birth (newborn), and at 1, 3, 6, 9, and 18 months of age. The collagen network was assessed biochemically (collagen content, enzymatic and non-enzymatic cross-links) in specimens from the mid-diaphysis of the tibia and femur and biomechanically (tensile testing) from decalcified whole tibia specimens. The mineralized matrix was analyzed using pQCT and 3-point bend tests from intact femur specimens. The collagen content and the Young's modulus of the collagen matrix increased significantly until the rabbits were 3 months old, and thereafter remained stable. The amount of HP and LP collagen cross-links increased continuously from newborn to 18 months of age, whereas PEN cross-links increased after 6 months of age. Bone mineral density and the Young's modulus of the mineralized bone increased until the rabbits were at least 6 months old. We concluded that substantial changes take place during the normal process of development in both the biochemical and biomechanical properties of rabbit cortical bone. In cortical bone, the collagen network reaches its mature composition and mechanical strength prior to the mineralized matrix. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277184
- author
- Isaksson, Hanna
LU
; Harjula, Terhi
; Koistinen, Arto
; Iivarinen, Jaakko
; Seppänen, Kari
; Arikoski, Jari
; Brama, Pieter
; Jurvelin, Jukka
and Helminen, Heikki
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Orthopaedic Research
- volume
- 28
- issue
- 12
- pages
- 1626 - 1633
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:78149473425
- pmid:20540098
- ISSN
- 1554-527X
- DOI
- 10.1002/jor.21186
- language
- English
- LU publication?
- no
- id
- 1cd58ffa-ac71-4c7d-9da5-9b752f42d7d3 (old id 2277184)
- date added to LUP
- 2016-04-01 10:44:20
- date last changed
- 2023-08-31 10:18:40
@article{1cd58ffa-ac71-4c7d-9da5-9b752f42d7d3,
abstract = {{Abstract in Undetermined<br/>We characterized the composition and mechanical properties of cortical bone during maturation and growth and in adult life in the rabbit. We hypothesized that the collagen network develops earlier than the mineralized matrix. Growth was monitored, and the rabbits were euthanized at birth (newborn), and at 1, 3, 6, 9, and 18 months of age. The collagen network was assessed biochemically (collagen content, enzymatic and non-enzymatic cross-links) in specimens from the mid-diaphysis of the tibia and femur and biomechanically (tensile testing) from decalcified whole tibia specimens. The mineralized matrix was analyzed using pQCT and 3-point bend tests from intact femur specimens. The collagen content and the Young's modulus of the collagen matrix increased significantly until the rabbits were 3 months old, and thereafter remained stable. The amount of HP and LP collagen cross-links increased continuously from newborn to 18 months of age, whereas PEN cross-links increased after 6 months of age. Bone mineral density and the Young's modulus of the mineralized bone increased until the rabbits were at least 6 months old. We concluded that substantial changes take place during the normal process of development in both the biochemical and biomechanical properties of rabbit cortical bone. In cortical bone, the collagen network reaches its mature composition and mechanical strength prior to the mineralized matrix.}},
author = {{Isaksson, Hanna and Harjula, Terhi and Koistinen, Arto and Iivarinen, Jaakko and Seppänen, Kari and Arikoski, Jari and Brama, Pieter and Jurvelin, Jukka and Helminen, Heikki}},
issn = {{1554-527X}},
language = {{eng}},
number = {{12}},
pages = {{1626--1633}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Orthopaedic Research}},
title = {{Timing of collagen and mineral deposition in cortical bone and effect on tissue properties}},
url = {{http://dx.doi.org/10.1002/jor.21186}},
doi = {{10.1002/jor.21186}},
volume = {{28}},
year = {{2010}},
}