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Reproducibility and agreement of micro-CT and histomorphometry in human trabecular bone with different metabolic status

Tamminen, Inari; Isaksson, Hanna LU ; Aula, Antti; Honkanen, Ero; Jurvelin, Jukka and Kröger, Heikki (2011) In Journal of Bone and Mineral Metabolism 29(4). p.442-448
Abstract (Swedish)
Abstract in Undetermined

The use of micro-computed tomography (micro-CT) to study bone microstructure is continuously increasing. Thus, it is important to ensure that micro-CT can differentiate healthy and pathological bone. This study aimed to determine whether the reproducibility of bone histomorphometry and micro-CT, and agreement between the techniques, vary in bone samples with different metabolic status. Iliac crest biopsies (n = 36) were obtained from healthy subjects (n = 10) and from patients with osteoporosis (OP) (n = 15) or renal osteodystrophy (ROD) (n = 11). Micro-CT and histomorphometry analyses were repeated twice. Results were analyzed in separate groups and after pooling the data. Bone histomorphometry... (More)
Abstract in Undetermined

The use of micro-computed tomography (micro-CT) to study bone microstructure is continuously increasing. Thus, it is important to ensure that micro-CT can differentiate healthy and pathological bone. This study aimed to determine whether the reproducibility of bone histomorphometry and micro-CT, and agreement between the techniques, vary in bone samples with different metabolic status. Iliac crest biopsies (n = 36) were obtained from healthy subjects (n = 10) and from patients with osteoporosis (OP) (n = 15) or renal osteodystrophy (ROD) (n = 11). Micro-CT and histomorphometry analyses were repeated twice. Results were analyzed in separate groups and after pooling the data. Bone histomorphometry detected generally known differences between the diseases, whereas micro-CT did not detect differences between normal and ROD samples as effectively. Repeated measurements for BV/TV, Tb.Th, Tb.N, and Tb.Sp exhibited linear correlation coefficients (ρ) of 0.87–0.92 [coefficients of variations (CV), 8.3–27.2%] for histomorphometry and of 0.66–0.94 (CV, 4.4–23.4%) for micro-CT. There were no significant differences in reproducibility among samples from different study groups. Correlations between BV/TV (micro-CT) and mineralized bone volume (Md.V/TV, histomorphometry) were weaker than between BV/TV (micro-CT) and BV/TV (histomorphometry). When comparing the techniques, BV/TV, Tb.Th, and Tb.N displayed moderate correlations (ρ = 0.39–0.62, P < 0.05), and the agreement for BV/TV was highest in OP samples. The agreement between the techniques using clinical bone samples was moderate. Especially, micro-CT was less effective than bone histomorphometry in differentiating ROD from normal samples. The reproducibility was not affected by the health status of bone. Histomorphometry is still needed in clinical practice to study the remodeling balance in bone, and the methods are complementary. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bone and Mineral Metabolism
volume
29
issue
4
pages
442 - 448
publisher
Springer
external identifiers
  • scopus:80052429654
ISSN
0914-8779
DOI
10.1007/s00774-010-0236-6
language
English
LU publication?
no
id
3467f123-a814-4581-8557-6abcb54463c6 (old id 2277211)
date added to LUP
2012-01-27 09:24:42
date last changed
2017-11-05 03:23:27
@article{3467f123-a814-4581-8557-6abcb54463c6,
  abstract     = {<b>Abstract in Undetermined</b><br/><br>
The use of micro-computed tomography (micro-CT) to study bone microstructure is continuously increasing. Thus, it is important to ensure that micro-CT can differentiate healthy and pathological bone. This study aimed to determine whether the reproducibility of bone histomorphometry and micro-CT, and agreement between the techniques, vary in bone samples with different metabolic status. Iliac crest biopsies (n = 36) were obtained from healthy subjects (n = 10) and from patients with osteoporosis (OP) (n = 15) or renal osteodystrophy (ROD) (n = 11). Micro-CT and histomorphometry analyses were repeated twice. Results were analyzed in separate groups and after pooling the data. Bone histomorphometry detected generally known differences between the diseases, whereas micro-CT did not detect differences between normal and ROD samples as effectively. Repeated measurements for BV/TV, Tb.Th, Tb.N, and Tb.Sp exhibited linear correlation coefficients (ρ) of 0.87–0.92 [coefficients of variations (CV), 8.3–27.2%] for histomorphometry and of 0.66–0.94 (CV, 4.4–23.4%) for micro-CT. There were no significant differences in reproducibility among samples from different study groups. Correlations between BV/TV (micro-CT) and mineralized bone volume (Md.V/TV, histomorphometry) were weaker than between BV/TV (micro-CT) and BV/TV (histomorphometry). When comparing the techniques, BV/TV, Tb.Th, and Tb.N displayed moderate correlations (ρ = 0.39–0.62, P &lt; 0.05), and the agreement for BV/TV was highest in OP samples. The agreement between the techniques using clinical bone samples was moderate. Especially, micro-CT was less effective than bone histomorphometry in differentiating ROD from normal samples. The reproducibility was not affected by the health status of bone. Histomorphometry is still needed in clinical practice to study the remodeling balance in bone, and the methods are complementary.},
  author       = {Tamminen, Inari and Isaksson, Hanna and Aula, Antti and Honkanen, Ero and Jurvelin, Jukka and Kröger, Heikki},
  issn         = {0914-8779},
  language     = {eng},
  number       = {4},
  pages        = {442--448},
  publisher    = {Springer},
  series       = {Journal of Bone and Mineral Metabolism},
  title        = {Reproducibility and agreement of micro-CT and histomorphometry in human trabecular bone with different metabolic status},
  url          = {http://dx.doi.org/10.1007/s00774-010-0236-6},
  volume       = {29},
  year         = {2011},
}