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Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation

Kobrina, Yevgeniya; Isaksson, Hanna LU ; Sinisaari, Miikka; Rieppo, Lassi; Brama, Pieter; van Weeren, Rene; Helminen, Heikki; Jurvelin, Jukka and Saarakkala, Simo (2010) In Journal of Biomedical Optics 15(6).
Abstract
The collagen phase in bone is known to undergo major changes during growth and maturation. The objective of this study is to clarify whether Fourier transform infrared (FTIR) microspectroscopy, coupled with cluster analysis, can detect quantitative and qualitative changes in the collagen matrix of subchondral bone in horses during maturation and growth. Equine subchondral bone samples (n = 29) from the proximal joint surface of the first phalanx are prepared from two sites subjected to different loading conditions. Three age groups are studied: newborn (0 days old), immature (5 to 11 months old), and adult (6 to 10 years old) horses. Spatial collagen content and collagen cross-link ratio are quantified from the spectra. Additionally,... (More)
The collagen phase in bone is known to undergo major changes during growth and maturation. The objective of this study is to clarify whether Fourier transform infrared (FTIR) microspectroscopy, coupled with cluster analysis, can detect quantitative and qualitative changes in the collagen matrix of subchondral bone in horses during maturation and growth. Equine subchondral bone samples (n = 29) from the proximal joint surface of the first phalanx are prepared from two sites subjected to different loading conditions. Three age groups are studied: newborn (0 days old), immature (5 to 11 months old), and adult (6 to 10 years old) horses. Spatial collagen content and collagen cross-link ratio are quantified from the spectra. Additionally, normalized second derivative spectra of samples are clustered using the k-means clustering algorithm. In quantitative analysis, collagen content in the subchondral bone increases rapidly between the newborn and immature horses. The collagen cross-link ratio increases significantly with age. In qualitative analysis, clustering is able to separate newborn and adult samples into two different groups. The immature samples display some nonhomogeneity. In conclusion, this is the first study showing that FTIR spectral imaging combined with clustering techniques can detect quantitative and qualitative changes in the collagen matrix of subchondral bone during growth and maturation. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biomedical Optics
volume
15
issue
6
publisher
Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
external identifiers
  • scopus:78349243168
ISSN
1083-3668
DOI
10.1117/1.3512177
language
English
LU publication?
no
id
59d03ae8-ef95-4b92-93c5-c46eed85bcfa (old id 2277217)
date added to LUP
2012-01-27 09:17:00
date last changed
2018-05-29 10:11:59
@article{59d03ae8-ef95-4b92-93c5-c46eed85bcfa,
  abstract     = {The collagen phase in bone is known to undergo major changes during growth and maturation. The objective of this study is to clarify whether Fourier transform infrared (FTIR) microspectroscopy, coupled with cluster analysis, can detect quantitative and qualitative changes in the collagen matrix of subchondral bone in horses during maturation and growth. Equine subchondral bone samples (n = 29) from the proximal joint surface of the first phalanx are prepared from two sites subjected to different loading conditions. Three age groups are studied: newborn (0 days old), immature (5 to 11 months old), and adult (6 to 10 years old) horses. Spatial collagen content and collagen cross-link ratio are quantified from the spectra. Additionally, normalized second derivative spectra of samples are clustered using the k-means clustering algorithm. In quantitative analysis, collagen content in the subchondral bone increases rapidly between the newborn and immature horses. The collagen cross-link ratio increases significantly with age. In qualitative analysis, clustering is able to separate newborn and adult samples into two different groups. The immature samples display some nonhomogeneity. In conclusion, this is the first study showing that FTIR spectral imaging combined with clustering techniques can detect quantitative and qualitative changes in the collagen matrix of subchondral bone during growth and maturation.},
  articleno    = {067003},
  author       = {Kobrina, Yevgeniya and Isaksson, Hanna and Sinisaari, Miikka and Rieppo, Lassi and Brama, Pieter and van Weeren, Rene and Helminen, Heikki and Jurvelin, Jukka and Saarakkala, Simo},
  issn         = {1083-3668},
  language     = {eng},
  number       = {6},
  publisher    = {Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society},
  series       = {Journal of Biomedical Optics},
  title        = {Infrared spectroscopy reveals both qualitative and quantitative differences in equine subchondral bone during maturation},
  url          = {http://dx.doi.org/10.1117/1.3512177},
  volume       = {15},
  year         = {2010},
}