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NMR relaxation times of trabecular bone - reproducibility, relationships to tissue structure and effects of sample freezing

Pranter, Viktoria; Isaksson, Hanna LU ; Närväinen, Johanna; Lammentausta, Eveliina; Nissi, Mikko; Avela, Janne; Gröhn, Olli and Jurvelin, Jukka (2010) In Physics in Medicine and Biology 55(23). p.7363-7555
Abstract (Swedish)
Abstract in Undetermined

Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy... (More)
Abstract in Undetermined

Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy before and after freezing. The reproducibility of NMR relaxation parameters, as expressed by the coefficient of variation, ranged from 3.1% to 27.9%. In fresh samples, some correlations between NMR and structural parameters (Tb.N, Tb.Sp) were significant (e.g. the relaxation rate for T(2) of fat versus Tb.Sp: r = -0.716, p < 0.01). Freezing did not significantly change the NMR relaxation times but the correlations between relaxation parameters and the μCT structural parameters were not statistically significant after freezing, suggesting some nonsystematic alterations of the marrow structure. Therefore, the use of frozen bone samples for NMR relaxation studies may provide inferior information about the trabecular bone structure. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Physics in Medicine and Biology
volume
55
issue
23
pages
7363 - 7555
publisher
IOP Publishing
external identifiers
  • scopus:78650064279
ISSN
1361-6560
DOI
10.1088/0031-9155/55/23/012
language
English
LU publication?
no
id
b12ac467-cf62-463e-bfd1-552402217246 (old id 2277238)
date added to LUP
2012-01-27 09:03:03
date last changed
2018-05-29 11:41:40
@article{b12ac467-cf62-463e-bfd1-552402217246,
  abstract     = {<b>Abstract in Undetermined</b><br/><br>
Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy before and after freezing. The reproducibility of NMR relaxation parameters, as expressed by the coefficient of variation, ranged from 3.1% to 27.9%. In fresh samples, some correlations between NMR and structural parameters (Tb.N, Tb.Sp) were significant (e.g. the relaxation rate for T(2) of fat versus Tb.Sp: r = -0.716, p &lt; 0.01). Freezing did not significantly change the NMR relaxation times but the correlations between relaxation parameters and the μCT structural parameters were not statistically significant after freezing, suggesting some nonsystematic alterations of the marrow structure. Therefore, the use of frozen bone samples for NMR relaxation studies may provide inferior information about the trabecular bone structure.},
  author       = {Pranter, Viktoria and Isaksson, Hanna and Närväinen, Johanna and Lammentausta, Eveliina and Nissi, Mikko and Avela, Janne and Gröhn, Olli and Jurvelin, Jukka},
  issn         = {1361-6560},
  language     = {eng},
  number       = {23},
  pages        = {7363--7555},
  publisher    = {IOP Publishing},
  series       = {Physics in Medicine and Biology},
  title        = {NMR relaxation times of trabecular bone - reproducibility, relationships to tissue structure and effects of sample freezing},
  url          = {http://dx.doi.org/10.1088/0031-9155/55/23/012},
  volume       = {55},
  year         = {2010},
}