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An oat branmeal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects

Ulmius, Matilda LU ; Johansson Persson, Anna LU ; Krogh, Morten LU ; Olsson, Peter LU and Önning, Gunilla LU (2011) In Genes & Nutrition 6(4). p.429-439
Abstract
The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed... (More)
The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed genes in response either to meal intake or meal content, and enrichment analysis was used to identify functional gene sets responding to meal intake and specifically to oat bran intake. Meal intake as such affected gene expression for genes mainly involved in metabolic stress; indicating increased inflammation due to the switch from fasting to fed state. The oat bran meal affected gene sets associated with a lower insulin level, compared with the control meal. The gene sets included genes involved in insulin secretion and β-cell development, but also protein synthesis and genes related to cancer diseases. The oat bran meal also significantly lowered postprandial blood insulin IAUC compared to control. Further studies are needed to compare these acute effects with the long-term health effects of oat bran. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Oats Microarray gene expression Peripheral mononuclear blood cells Postprandial response
in
Genes & Nutrition
volume
6
issue
4
pages
429 - 439
publisher
New Century Health Publishers
external identifiers
  • wos:000300099500009
  • scopus:81755179422
ISSN
1555-8932
DOI
10.1007/s12263-011-0236-8
language
English
LU publication?
yes
id
ed64bdec-bc21-46f9-9216-c514c5725fd7 (old id 2277733)
date added to LUP
2012-01-09 09:11:11
date last changed
2017-09-03 03:12:04
@article{ed64bdec-bc21-46f9-9216-c514c5725fd7,
  abstract     = {The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed genes in response either to meal intake or meal content, and enrichment analysis was used to identify functional gene sets responding to meal intake and specifically to oat bran intake. Meal intake as such affected gene expression for genes mainly involved in metabolic stress; indicating increased inflammation due to the switch from fasting to fed state. The oat bran meal affected gene sets associated with a lower insulin level, compared with the control meal. The gene sets included genes involved in insulin secretion and β-cell development, but also protein synthesis and genes related to cancer diseases. The oat bran meal also significantly lowered postprandial blood insulin IAUC compared to control. Further studies are needed to compare these acute effects with the long-term health effects of oat bran.},
  author       = {Ulmius, Matilda and Johansson Persson, Anna and Krogh, Morten and Olsson, Peter and Önning, Gunilla},
  issn         = {1555-8932},
  keyword      = {Oats Microarray gene expression Peripheral mononuclear blood cells Postprandial response},
  language     = {eng},
  number       = {4},
  pages        = {429--439},
  publisher    = {New Century Health Publishers},
  series       = {Genes & Nutrition},
  title        = {An oat branmeal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects},
  url          = {http://dx.doi.org/10.1007/s12263-011-0236-8},
  volume       = {6},
  year         = {2011},
}