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Organization of Bacteriochlorophylls in Individual Chlorosomes from Chlorobaculum tepidum Studied by 2-Dimensional Polarization Fluorescence Microscopy

Tian, Yuxi LU ; Camacho Dejay, Rafael LU ; Thomsson, Daniel LU ; Reus, Michael; Holzwarth, Alfred R. and Scheblykin, Ivan LU (2011) In Journal of the American Chemical Society 133(43). p.17192-17199
Abstract
Chlorosomes are the largest and most efficient natural light-harvesting systems and contain supramolecular assemblies of bacteriochlorophylls that are organized without proteins. Despite a recent structure determination for chlorosomes from Chlorobaculum tepidum (Ganapathy et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 8525), the issue of a possible large structural disorder is still discussed controversially. We have studied individual chlorosomes prepared under very carefully controlled growth condition by a novel 2-dimensional polarization single molecule imaging technique giving polarization information for both fluorescence excitation and emission simultaneously. Contrary to the existing literature data, the polarization degree or... (More)
Chlorosomes are the largest and most efficient natural light-harvesting systems and contain supramolecular assemblies of bacteriochlorophylls that are organized without proteins. Despite a recent structure determination for chlorosomes from Chlorobaculum tepidum (Ganapathy et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 8525), the issue of a possible large structural disorder is still discussed controversially. We have studied individual chlorosomes prepared under very carefully controlled growth condition by a novel 2-dimensional polarization single molecule imaging technique giving polarization information for both fluorescence excitation and emission simultaneously. Contrary to the existing literature data, the polarization degree or modulation depth (M) for both excitation (absorption) and emission (fluorescence) showed extremely narrow distributions. The fluorescence was always highly polarized with M approximate to 0.77, independent of the excitation wavelength. Moreover, the fluorescence spectra of individual chlorosomes were identical within the error limits. These results lead us to conclude that all chlorosomes possess the same type of internal organization in terms of the arrangement of the bacteriochlorophyll c transition dipole moments and their total excitonic transition dipole possess a cylindrical symmetry in agreement with the previously suggested concentric multitubular chlorophyll aggregate organization (Ganapathy et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 8525). (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
133
issue
43
pages
17192 - 17199
publisher
The American Chemical Society
external identifiers
  • wos:000297380900022
  • scopus:80054987373
ISSN
1520-5126
DOI
10.1021/ja2019959
language
English
LU publication?
yes
id
22f8a215-0693-412c-9163-95d760806252 (old id 2291732)
date added to LUP
2012-01-11 12:46:52
date last changed
2017-09-03 04:12:07
@article{22f8a215-0693-412c-9163-95d760806252,
  abstract     = {Chlorosomes are the largest and most efficient natural light-harvesting systems and contain supramolecular assemblies of bacteriochlorophylls that are organized without proteins. Despite a recent structure determination for chlorosomes from Chlorobaculum tepidum (Ganapathy et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 8525), the issue of a possible large structural disorder is still discussed controversially. We have studied individual chlorosomes prepared under very carefully controlled growth condition by a novel 2-dimensional polarization single molecule imaging technique giving polarization information for both fluorescence excitation and emission simultaneously. Contrary to the existing literature data, the polarization degree or modulation depth (M) for both excitation (absorption) and emission (fluorescence) showed extremely narrow distributions. The fluorescence was always highly polarized with M approximate to 0.77, independent of the excitation wavelength. Moreover, the fluorescence spectra of individual chlorosomes were identical within the error limits. These results lead us to conclude that all chlorosomes possess the same type of internal organization in terms of the arrangement of the bacteriochlorophyll c transition dipole moments and their total excitonic transition dipole possess a cylindrical symmetry in agreement with the previously suggested concentric multitubular chlorophyll aggregate organization (Ganapathy et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 8525).},
  author       = {Tian, Yuxi and Camacho Dejay, Rafael and Thomsson, Daniel and Reus, Michael and Holzwarth, Alfred R. and Scheblykin, Ivan},
  issn         = {1520-5126},
  language     = {eng},
  number       = {43},
  pages        = {17192--17199},
  publisher    = {The American Chemical Society},
  series       = {Journal of the American Chemical Society},
  title        = {Organization of Bacteriochlorophylls in Individual Chlorosomes from Chlorobaculum tepidum Studied by 2-Dimensional Polarization Fluorescence Microscopy},
  url          = {http://dx.doi.org/10.1021/ja2019959},
  volume       = {133},
  year         = {2011},
}