Altered processing of human insulin by B lymphocytes from an immunologically insulinresistant type I diabetic patient
(1991) In Journal of Autoimmunity 4(2). p.277-289- Abstract
Immunologically insulin resistant (IIR) type I diabetic patients possess significantly elevated levels of anti-insulin serum autoantibodies. We investigated whether altered insulin processing by B lymphocytes contributes to this form of insulin resistance. A comparison was made of the 125I-labelled human insulin (HI) peptides processed by Epstein-Barr virus (EBV)-transformed B lymphocytes derived from HLA-identical and nonidentical IIR with non-IIR type I diabetic patients on insulin therapy and healthy non-diabetic individuals. Several peptides detected in the extracellular, membrane-associated and intracellular compartments of B cells from an IIR type I diabetic patient differed from those found in the corresponding... (More)
Immunologically insulin resistant (IIR) type I diabetic patients possess significantly elevated levels of anti-insulin serum autoantibodies. We investigated whether altered insulin processing by B lymphocytes contributes to this form of insulin resistance. A comparison was made of the 125I-labelled human insulin (HI) peptides processed by Epstein-Barr virus (EBV)-transformed B lymphocytes derived from HLA-identical and nonidentical IIR with non-IIR type I diabetic patients on insulin therapy and healthy non-diabetic individuals. Several peptides detected in the extracellular, membrane-associated and intracellular compartments of B cells from an IIR type I diabetic patient differed from those found in the corresponding compartments of B cells from two non-IIR type I diabetic patients and two normal individuals. These data suggest that HI is processed differently by B cells from an IIR type I diabetic patient compared with B cells from non-IIR type I diabetic patients and normal individuals. Further, we found that two of the five plasma-membrane associated processed HI peptides on the IIR patients' B cells were absent from the membrane compartments of the other B cell lines examined. Thus, it is possible that one or both of these peptides, unique to the IIR patients' B cells, consist of an immundominant epitope(s) that stimulates the production of insulin autoantibodies which mediate the onset of IIR in type I diabetes.
(Less)
- author
- Semple, John W. LU and Delovitch, Terry L.
- publishing date
- 1991-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Autoimmunity
- volume
- 4
- issue
- 2
- pages
- 277 - 289
- publisher
- Elsevier
- external identifiers
-
- pmid:1652968
- scopus:0025897055
- ISSN
- 0896-8411
- DOI
- 10.1016/0896-8411(91)90024-7
- language
- English
- LU publication?
- no
- id
- 229a267f-d2ee-4f94-bde2-125205cb701e
- date added to LUP
- 2019-12-03 10:35:38
- date last changed
- 2024-01-02 01:35:33
@article{229a267f-d2ee-4f94-bde2-125205cb701e,
abstract = {{<p>Immunologically insulin resistant (IIR) type I diabetic patients possess significantly elevated levels of anti-insulin serum autoantibodies. We investigated whether altered insulin processing by B lymphocytes contributes to this form of insulin resistance. A comparison was made of the <sup>125</sup>I-labelled human insulin (HI) peptides processed by Epstein-Barr virus (EBV)-transformed B lymphocytes derived from HLA-identical and nonidentical IIR with non-IIR type I diabetic patients on insulin therapy and healthy non-diabetic individuals. Several peptides detected in the extracellular, membrane-associated and intracellular compartments of B cells from an IIR type I diabetic patient differed from those found in the corresponding compartments of B cells from two non-IIR type I diabetic patients and two normal individuals. These data suggest that HI is processed differently by B cells from an IIR type I diabetic patient compared with B cells from non-IIR type I diabetic patients and normal individuals. Further, we found that two of the five plasma-membrane associated processed HI peptides on the IIR patients' B cells were absent from the membrane compartments of the other B cell lines examined. Thus, it is possible that one or both of these peptides, unique to the IIR patients' B cells, consist of an immundominant epitope(s) that stimulates the production of insulin autoantibodies which mediate the onset of IIR in type I diabetes.</p>}},
author = {{Semple, John W. and Delovitch, Terry L.}},
issn = {{0896-8411}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{277--289}},
publisher = {{Elsevier}},
series = {{Journal of Autoimmunity}},
title = {{Altered processing of human insulin by B lymphocytes from an immunologically insulinresistant type I diabetic patient}},
url = {{http://dx.doi.org/10.1016/0896-8411(91)90024-7}},
doi = {{10.1016/0896-8411(91)90024-7}},
volume = {{4}},
year = {{1991}},
}