Investigation into A antigen expression on O-2 heterozygous group O-labeled red blood cell units
Yazer, Mark H. ; Hult, Annika LU ; Hellberg, Åsa LU ; Hosseini Maaf, Bahram LU ; Palcic, Monica M. and Olsson, Martin L LU
(2008)
In Transfusion
48(8).
p.1650-1657
- Abstract
- BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The... (More)
- BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The following tests were performed on randomly selected O-2 samples (number): adsorption-elution with anti-A (3), flow cytometry (15), plasma enzyme activity (4), and attempts to convert group O RBCs to A (2) with O-2 plasma and titration of plasma anti-A/-A(1) (3). RESULTS: Forty O-2-heterozygous donors were identified (5.1%). Adsorption-elution and sensitive flow cytometry did not reveal A antigens on O-2 RBCs. Plasma enzyme analysis failed to show GTA activity above baseline; O-2 plasma was unable to add measurable A antigens to O RBCs. Titers of anti-A/-A(1) appeared reduced in O-2 plasma but did not cause ABO typing discrepancies. No immediate hemolysis or adverse reactions were reported following transfusion of O-2 RBCs to six evaluable group O recipients. CONCLUSIONS: Other than lower plasma anti-A titers, GTA activity was not found in these O-2 samples. Neither automated blood grouping discrepancies nor clinical problems related to transfusing these O-2 units were observed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1253566
- author
- Yazer, Mark H.
; Hult, Annika
LU
; Hellberg, Åsa
LU
; Hosseini Maaf, Bahram
LU
; Palcic, Monica M.
and Olsson, Martin L
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion
- volume
- 48
- issue
- 8
- pages
- 1650 - 1657
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000258077800019
- scopus:48249146230
- pmid:18482182
- ISSN
- 1537-2995
- DOI
- 10.1111/j.1537-2995.2008.01732.x
- language
- English
- LU publication?
- yes
- id
- 22c9c15f-66a5-4711-be39-3bad246d197b (old id 1253566)
- date added to LUP
- 2016-04-01 14:55:29
- date last changed
- 2022-01-28 03:10:04
@article{22c9c15f-66a5-4711-be39-3bad246d197b,
abstract = {{BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The following tests were performed on randomly selected O-2 samples (number): adsorption-elution with anti-A (3), flow cytometry (15), plasma enzyme activity (4), and attempts to convert group O RBCs to A (2) with O-2 plasma and titration of plasma anti-A/-A(1) (3). RESULTS: Forty O-2-heterozygous donors were identified (5.1%). Adsorption-elution and sensitive flow cytometry did not reveal A antigens on O-2 RBCs. Plasma enzyme analysis failed to show GTA activity above baseline; O-2 plasma was unable to add measurable A antigens to O RBCs. Titers of anti-A/-A(1) appeared reduced in O-2 plasma but did not cause ABO typing discrepancies. No immediate hemolysis or adverse reactions were reported following transfusion of O-2 RBCs to six evaluable group O recipients. CONCLUSIONS: Other than lower plasma anti-A titers, GTA activity was not found in these O-2 samples. Neither automated blood grouping discrepancies nor clinical problems related to transfusing these O-2 units were observed.}},
author = {{Yazer, Mark H. and Hult, Annika and Hellberg, Åsa and Hosseini Maaf, Bahram and Palcic, Monica M. and Olsson, Martin L}},
issn = {{1537-2995}},
language = {{eng}},
number = {{8}},
pages = {{1650--1657}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion}},
title = {{Investigation into A antigen expression on O-2 heterozygous group O-labeled red blood cell units}},
url = {{http://dx.doi.org/10.1111/j.1537-2995.2008.01732.x}},
doi = {{10.1111/j.1537-2995.2008.01732.x}},
volume = {{48}},
year = {{2008}},
}