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Köpek periferal kan örneklerinde Neospora caninum ve Toxoplasma gondii takizoitlerinin TaqMan prob bazli{dotless} Real Time PCR ile araşti{dotless}ri{dotless}lmasi{dotless}

Düzlü, Önder ; Inci, Abdullah ; Yildirim, Alparslan ; Önder, Zuhal and Çiloǧlu, Arif LU (2014) In Ankara Universitesi Veteriner Fakultesi Dergisi 61(4). p.283-288
Abstract

This study was carried out to investigate Neospora caninum and Toxoplasma gondii tachyzoites in total of 400 whole peripheral blood samples with EDTA which were collected from the clinically healthy dogs in Kayseri region. Following the isolation of genomic DNA from the blood samples, spectrophotometric measurements of the obtained genomic DNAs were performed and appropriate concentrations were prepared for Real Time PCR (qPCR). qPCR analyses of the samples were conducted using the specific primers and probes that target the gene regions NC5 for N. caninum and RE for T. gondii. Out of 400 blood samples, 15 (3.8%) were found to be infected with N. caninum while no T. gondii positivity was determined in the examined samples. In... (More)

This study was carried out to investigate Neospora caninum and Toxoplasma gondii tachyzoites in total of 400 whole peripheral blood samples with EDTA which were collected from the clinically healthy dogs in Kayseri region. Following the isolation of genomic DNA from the blood samples, spectrophotometric measurements of the obtained genomic DNAs were performed and appropriate concentrations were prepared for Real Time PCR (qPCR). qPCR analyses of the samples were conducted using the specific primers and probes that target the gene regions NC5 for N. caninum and RE for T. gondii. Out of 400 blood samples, 15 (3.8%) were found to be infected with N. caninum while no T. gondii positivity was determined in the examined samples. In conclusion, some molecular epidemiological data about N. caninum and T. gondii infections dogs in Kayseri region were firstly demonstrated in Turkey with this study.

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author
; ; ; and
alternative title
Investigation of Neospora caninum and Toxoplasma gondii tachyzoites in peripheral blood samples of dogs by TaqMan probe based Real Time PCR
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dog, Neospora caninum, Real Time PCR, Tachyzoit, Toxoplasma gondii
in
Ankara Universitesi Veteriner Fakultesi Dergisi
volume
61
issue
4
pages
6 pages
publisher
Chartered Institution of Building Services Engineers
external identifiers
  • scopus:84904541005
ISSN
1300-0861
DOI
10.1501/Vetfak_0000002643
language
Turkish
LU publication?
no
id
22dc994c-7947-46ab-ae6d-a227a66cd29c
date added to LUP
2017-12-04 15:17:16
date last changed
2022-03-24 22:42:15
@article{22dc994c-7947-46ab-ae6d-a227a66cd29c,
  abstract     = {{<p>This study was carried out to investigate Neospora caninum and Toxoplasma gondii tachyzoites in total of 400 whole peripheral blood samples with EDTA which were collected from the clinically healthy dogs in Kayseri region. Following the isolation of genomic DNA from the blood samples, spectrophotometric measurements of the obtained genomic DNAs were performed and appropriate concentrations were prepared for Real Time PCR (qPCR). qPCR analyses of the samples were conducted using the specific primers and probes that target the gene regions NC5 for N. caninum and RE for T. gondii. Out of 400 blood samples, 15 (3.8%) were found to be infected with N. caninum while no T. gondii positivity was determined in the examined samples. In conclusion, some molecular epidemiological data about N. caninum and T. gondii infections dogs in Kayseri region were firstly demonstrated in Turkey with this study.</p>}},
  author       = {{Düzlü, Önder and Inci, Abdullah and Yildirim, Alparslan and Önder, Zuhal and Çiloǧlu, Arif}},
  issn         = {{1300-0861}},
  keywords     = {{Dog; Neospora caninum; Real Time PCR; Tachyzoit; Toxoplasma gondii}},
  language     = {{tur}},
  number       = {{4}},
  pages        = {{283--288}},
  publisher    = {{Chartered Institution of Building Services Engineers}},
  series       = {{Ankara Universitesi Veteriner Fakultesi Dergisi}},
  title        = {{Köpek periferal kan örneklerinde Neospora caninum ve Toxoplasma gondii takizoitlerinin TaqMan prob bazli{dotless} Real Time PCR ile araşti{dotless}ri{dotless}lmasi{dotless}}},
  url          = {{http://dx.doi.org/10.1501/Vetfak_0000002643}},
  doi          = {{10.1501/Vetfak_0000002643}},
  volume       = {{61}},
  year         = {{2014}},
}