Non-genomic effects of androgens on isolated human vascular and nonvascular penile erectile tissue
(2008) In BJU International 101(1). p.71-74- Abstract
- OBJECTIVES To evaluate non-genomic effects of testosterone and dihydrotestosterone (DHT) on isolated human cavernosal arteries (HCA) and corpus cavernosum (HCC) using organ-bath studies and radio-immunoassays (RIA), as non-genomic effects of androgens are reported for vascular smooth musculature and there is evidence that the relaxant response involves a modulation of cyclic nucleotide tissue levels. MATERIALS AND METHODS The relaxation induced by the cumulative addition of testosterone and DHT (0.01-10 mu M) was studied using circular segments of HCA and strip preparations of HCC. To evaluate the effects of testosterone and DHT on tissue levels of cAMP and cGMP, specimens were exposed to increasing concentrations of the hormones.... (More)
- OBJECTIVES To evaluate non-genomic effects of testosterone and dihydrotestosterone (DHT) on isolated human cavernosal arteries (HCA) and corpus cavernosum (HCC) using organ-bath studies and radio-immunoassays (RIA), as non-genomic effects of androgens are reported for vascular smooth musculature and there is evidence that the relaxant response involves a modulation of cyclic nucleotide tissue levels. MATERIALS AND METHODS The relaxation induced by the cumulative addition of testosterone and DHT (0.01-10 mu M) was studied using circular segments of HCA and strip preparations of HCC. To evaluate the effects of testosterone and DHT on tissue levels of cAMP and cGMP, specimens were exposed to increasing concentrations of the hormones. Forskolin and sodium nitroprusside (SNP) served as reference compounds. RESULTS Testosterone and DHT dose-dependently reversed the noradrenaline-induced tension of vascular segments and HCC strips. At the maximum concentration, testosterone and DHT reduced the mean (SD) tension to 79.8 (4.43)% and 83.9 (10.94)%, respectively. SNP and forskolin significantly stimulated the production of cGMP and cAMP. No effects of testosterone and DHT on cGMP and cAMP levels were detected. CONCLUSION Rapid androgen-induced relaxation of HCA and HCC occurs via non-genomic mechanisms. In penile erectile tissue, non-genomic relaxant effects of testosterone and DHT are not mediated via modulation of cyclic nucleotide tissue levels. Additional studies are required to establish if non-genomic relaxant effects are important in ensuring a basal level of perfusion to maintain overall penile function. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/966398
- author
- Waldkirch, Eginhard ; Ucker, Stefan ; Schultheiss, Dirk ; Geismar, Ulrike ; Bruns, Carola ; Scheller, Friedemann ; Jonas, Udo ; Becker, Armin J. ; Stief, Christian G. and Hedlund, Petter LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- dihydrotestosterone, cavernosal arteries, corpus cavernosum, non-genomic effects, hypogonadism, testosterone
- in
- BJU International
- volume
- 101
- issue
- 1
- pages
- 71 - 74
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000251503000015
- scopus:36849018536
- pmid:17868421
- ISSN
- 1464-4096
- DOI
- 10.1111/j.1464-410X.2007.07182.x
- language
- English
- LU publication?
- yes
- id
- 231382c8-5370-4535-b9f0-4cfcf1ad4cab (old id 966398)
- date added to LUP
- 2016-04-01 12:32:33
- date last changed
- 2022-01-27 06:32:59
@article{231382c8-5370-4535-b9f0-4cfcf1ad4cab,
abstract = {{OBJECTIVES To evaluate non-genomic effects of testosterone and dihydrotestosterone (DHT) on isolated human cavernosal arteries (HCA) and corpus cavernosum (HCC) using organ-bath studies and radio-immunoassays (RIA), as non-genomic effects of androgens are reported for vascular smooth musculature and there is evidence that the relaxant response involves a modulation of cyclic nucleotide tissue levels. MATERIALS AND METHODS The relaxation induced by the cumulative addition of testosterone and DHT (0.01-10 mu M) was studied using circular segments of HCA and strip preparations of HCC. To evaluate the effects of testosterone and DHT on tissue levels of cAMP and cGMP, specimens were exposed to increasing concentrations of the hormones. Forskolin and sodium nitroprusside (SNP) served as reference compounds. RESULTS Testosterone and DHT dose-dependently reversed the noradrenaline-induced tension of vascular segments and HCC strips. At the maximum concentration, testosterone and DHT reduced the mean (SD) tension to 79.8 (4.43)% and 83.9 (10.94)%, respectively. SNP and forskolin significantly stimulated the production of cGMP and cAMP. No effects of testosterone and DHT on cGMP and cAMP levels were detected. CONCLUSION Rapid androgen-induced relaxation of HCA and HCC occurs via non-genomic mechanisms. In penile erectile tissue, non-genomic relaxant effects of testosterone and DHT are not mediated via modulation of cyclic nucleotide tissue levels. Additional studies are required to establish if non-genomic relaxant effects are important in ensuring a basal level of perfusion to maintain overall penile function.}},
author = {{Waldkirch, Eginhard and Ucker, Stefan and Schultheiss, Dirk and Geismar, Ulrike and Bruns, Carola and Scheller, Friedemann and Jonas, Udo and Becker, Armin J. and Stief, Christian G. and Hedlund, Petter}},
issn = {{1464-4096}},
keywords = {{dihydrotestosterone; cavernosal arteries; corpus cavernosum; non-genomic effects; hypogonadism; testosterone}},
language = {{eng}},
number = {{1}},
pages = {{71--74}},
publisher = {{Wiley-Blackwell}},
series = {{BJU International}},
title = {{Non-genomic effects of androgens on isolated human vascular and nonvascular penile erectile tissue}},
url = {{http://dx.doi.org/10.1111/j.1464-410X.2007.07182.x}},
doi = {{10.1111/j.1464-410X.2007.07182.x}},
volume = {{101}},
year = {{2008}},
}