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Assignment of the low-temperature fluorescence in oxygen-evolving Photosystem II

Krausz, E; Hughes, J L; Smith, P J; Pace, R J and Peterson Årsköld, Sindra LU (2005) In Photosynthesis Research 84(1-3). p.193-199
Abstract
Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in... (More)
Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in CP43 or CP47 is located within its inhomogeneous distribution. PS II fluorescence arises from CP43 and CP47 'slow-transfer' states, convolved by this dependence. At higher temperatures, thermally activated excitation transfer to the PS II charge-separating system bypasses such bottlenecks. As the charge-separating state of active PS II absorbs at >700 nm, PS II emission in the 680-700 nm region is unlikely to arise from reaction centre pigments. PS II emission at physiological temperatures is discussed in terms of these results. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fluorescence, fluorescence line narrowing, energy transfer, PSII
in
Photosynthesis Research
volume
84
issue
1-3
pages
193 - 199
publisher
Springer
external identifiers
  • wos:000230845200029
  • pmid:16049774
  • scopus:23444436729
ISSN
0166-8595
DOI
10.1007/s11120-004-7078-9
language
English
LU publication?
yes
id
4bf488a6-aee8-4a3e-8458-b90ec4441729 (old id 231414)
date added to LUP
2007-08-03 12:07:11
date last changed
2017-07-30 04:39:22
@article{4bf488a6-aee8-4a3e-8458-b90ec4441729,
  abstract     = {Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in CP43 or CP47 is located within its inhomogeneous distribution. PS II fluorescence arises from CP43 and CP47 'slow-transfer' states, convolved by this dependence. At higher temperatures, thermally activated excitation transfer to the PS II charge-separating system bypasses such bottlenecks. As the charge-separating state of active PS II absorbs at >700 nm, PS II emission in the 680-700 nm region is unlikely to arise from reaction centre pigments. PS II emission at physiological temperatures is discussed in terms of these results.},
  author       = {Krausz, E and Hughes, J L and Smith, P J and Pace, R J and Peterson Årsköld, Sindra},
  issn         = {0166-8595},
  keyword      = {fluorescence,fluorescence line narrowing,energy transfer,PSII},
  language     = {eng},
  number       = {1-3},
  pages        = {193--199},
  publisher    = {Springer},
  series       = {Photosynthesis Research},
  title        = {Assignment of the low-temperature fluorescence in oxygen-evolving Photosystem II},
  url          = {http://dx.doi.org/10.1007/s11120-004-7078-9},
  volume       = {84},
  year         = {2005},
}