Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD
(2005) In Photosynthesis Research 84(1-3). p.309-316- Abstract
- Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD)... (More)
- Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Q(y) region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/231420
- author
- Peterson Årsköld, Sindra LU ; Smith, PJ ; Shen, JR ; Pace, RJ and Krausz, E
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- chlorophyll, CD, electrochromic shift, MCD, P680, pea, pheophytin, spinach, PSII, Thermosynechococcus vulcanus, Synechocystis PCC 6803
- in
- Photosynthesis Research
- volume
- 84
- issue
- 1-3
- pages
- 309 - 316
- publisher
- Springer
- external identifiers
-
- wos:000230845200046
- scopus:23444460413
- ISSN
- 0166-8595
- DOI
- 10.1007/s11120-005-2135-6
- language
- English
- LU publication?
- yes
- id
- 1877ba6d-738f-40d7-9893-8287d2f459c1 (old id 231420)
- date added to LUP
- 2016-04-01 15:42:40
- date last changed
- 2022-01-28 06:43:55
@article{1877ba6d-738f-40d7-9893-8287d2f459c1, abstract = {{Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Q(y) region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.}}, author = {{Peterson Årsköld, Sindra and Smith, PJ and Shen, JR and Pace, RJ and Krausz, E}}, issn = {{0166-8595}}, keywords = {{chlorophyll; CD; electrochromic shift; MCD; P680; pea; pheophytin; spinach; PSII; Thermosynechococcus vulcanus; Synechocystis PCC 6803}}, language = {{eng}}, number = {{1-3}}, pages = {{309--316}}, publisher = {{Springer}}, series = {{Photosynthesis Research}}, title = {{Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD}}, url = {{http://dx.doi.org/10.1007/s11120-005-2135-6}}, doi = {{10.1007/s11120-005-2135-6}}, volume = {{84}}, year = {{2005}}, }