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Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD

Peterson Årsköld, Sindra LU ; Smith, PJ; Shen, JR; Pace, RJ and Krausz, E (2005) In Photosynthesis Research 84(1-3). p.309-316
Abstract
Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD)... (More)
Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Q(y) region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
chlorophyll, CD, electrochromic shift, MCD, P680, pea, pheophytin, spinach, PSII, Thermosynechococcus vulcanus, Synechocystis PCC 6803
in
Photosynthesis Research
volume
84
issue
1-3
pages
309 - 316
publisher
Springer
external identifiers
  • wos:000230845200046
  • scopus:23444460413
ISSN
0166-8595
DOI
10.1007/s11120-005-2135-6
language
English
LU publication?
yes
id
1877ba6d-738f-40d7-9893-8287d2f459c1 (old id 231420)
date added to LUP
2007-08-16 09:26:57
date last changed
2017-01-01 06:45:07
@article{1877ba6d-738f-40d7-9893-8287d2f459c1,
  abstract     = {Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from Q(A) reduction by 1.7-K illumination were recorded, and the Q(x) and Q(y) absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Q(x) transition is similar to 3 nm (100 cm(-1)supercript stop) to higher energy in cyanobacteria than in the plants. The predominant Q(y) shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Q(y) region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.},
  author       = {Peterson Årsköld, Sindra and Smith, PJ and Shen, JR and Pace, RJ and Krausz, E},
  issn         = {0166-8595},
  keyword      = {chlorophyll,CD,electrochromic shift,MCD,P680,pea,pheophytin,spinach,PSII,Thermosynechococcus vulcanus,Synechocystis PCC 6803},
  language     = {eng},
  number       = {1-3},
  pages        = {309--316},
  publisher    = {Springer},
  series       = {Photosynthesis Research},
  title        = {Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD},
  url          = {http://dx.doi.org/10.1007/s11120-005-2135-6},
  volume       = {84},
  year         = {2005},
}