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Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages

Brachvogel, B; Moch, H; Pausch, F; Schlotzer-Schrehardt, U; Hofmann, C; Hallmann, Rupert LU ; von der Mark, K; Winkler, T and Poschl, E (2005) In Development 132(11). p.2657-2668
Abstract
The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system. In this paper, the expression of an Anxa5-lacZ fusion gene was used to define the onset of expression in the vasculature and to characterize these Anxa5-lacZ-expressing vasculature-associated cells. After blastocyst implantation, Anxa5-lacZ-positive cells were first detected in extra-embryonic tissues and in angioblast progenitors forming the primary vascular plexus. Later, expression is highly restricted to perivascular cells in most blood vessels resembling pericytes or vascular smooth muscle cells. Viable Anxa5-lacZ(+) perivascular cells were isolated from embryos as well as adult brain meninges by... (More)
The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system. In this paper, the expression of an Anxa5-lacZ fusion gene was used to define the onset of expression in the vasculature and to characterize these Anxa5-lacZ-expressing vasculature-associated cells. After blastocyst implantation, Anxa5-lacZ-positive cells were first detected in extra-embryonic tissues and in angioblast progenitors forming the primary vascular plexus. Later, expression is highly restricted to perivascular cells in most blood vessels resembling pericytes or vascular smooth muscle cells. Viable Anxa5-lacZ(+) perivascular cells were isolated from embryos as well as adult brain meninges by specific staining with fluorescent X-gal substrates and cell-sorting. These purified lacZ(+) cells specifically express known markers of pericytes, but also markers characteristic for stem cell populations. In vitro and in vivo differentiation experiments show that this cell pool expresses early markers of chondrogenesis, is capable of forming a calcified matrix and differentiates into adipocytes. Hence, Anxa5 expression in perivascular cells from mouse defines a novel population of cells with a distinct developmental potential. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
perivascular cells, pericytes, annexin A5, adult stem cells
in
Development
volume
132
issue
11
pages
2657 - 2668
publisher
Society for International Development
external identifiers
  • wos:000230062800014
  • pmid:15857912
  • scopus:21244460348
ISSN
1477-9129
DOI
10.1242/dev.01846
language
English
LU publication?
yes
id
d282e91d-0e20-4646-8b22-b77b668300d7 (old id 233517)
date added to LUP
2007-08-22 08:34:14
date last changed
2017-04-09 03:29:06
@article{d282e91d-0e20-4646-8b22-b77b668300d7,
  abstract     = {The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system. In this paper, the expression of an Anxa5-lacZ fusion gene was used to define the onset of expression in the vasculature and to characterize these Anxa5-lacZ-expressing vasculature-associated cells. After blastocyst implantation, Anxa5-lacZ-positive cells were first detected in extra-embryonic tissues and in angioblast progenitors forming the primary vascular plexus. Later, expression is highly restricted to perivascular cells in most blood vessels resembling pericytes or vascular smooth muscle cells. Viable Anxa5-lacZ(+) perivascular cells were isolated from embryos as well as adult brain meninges by specific staining with fluorescent X-gal substrates and cell-sorting. These purified lacZ(+) cells specifically express known markers of pericytes, but also markers characteristic for stem cell populations. In vitro and in vivo differentiation experiments show that this cell pool expresses early markers of chondrogenesis, is capable of forming a calcified matrix and differentiates into adipocytes. Hence, Anxa5 expression in perivascular cells from mouse defines a novel population of cells with a distinct developmental potential.},
  author       = {Brachvogel, B and Moch, H and Pausch, F and Schlotzer-Schrehardt, U and Hofmann, C and Hallmann, Rupert and von der Mark, K and Winkler, T and Poschl, E},
  issn         = {1477-9129},
  keyword      = {perivascular cells,pericytes,annexin A5,adult stem cells},
  language     = {eng},
  number       = {11},
  pages        = {2657--2668},
  publisher    = {Society for International Development},
  series       = {Development},
  title        = {Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages},
  url          = {http://dx.doi.org/10.1242/dev.01846},
  volume       = {132},
  year         = {2005},
}