Differential expression of chemokine receptors on human IgA plus and IgG plus B cells
(2005) In Clinical and Experimental Immunology 141(2). p.279-287- Abstract
- Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+... (More)
- Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+ cells expressed CCR2, CCR3, or CCR9, and there was no difference in the expression of these receptors between IgA+ and IgG+ cells. In contrast, CCR4, CCR5, and CXCR3 was expressed on significantly more IgG+ than IgA+ cells. The function of chemokine receptors on memory B cells and ASC was then tested in the transwell system. IgG+ memory cells migrated to a higher extent than IgA+ cells towards the CXCR3 ligand CXCL11/I-TAC, while there was only a small migration towards the CCR4 ligand CCL17/TARC and the CCR9 ligand CCL25/TECK. ASC migrated poorly to all chemokines tested. In conclusion, this study shows that IgG+ and IgA+ memory B cells have a differential expression of the Th1 associated chemokine receptor CXCR3, as well as of CCR4 and CCR5. In contrast, none of the studied chemokine receptors was preferentially expressed by IgA+ cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/233527
- author
- Johansson, C ; Ahlstedt, I ; Furubacka, S ; Johnsson, E ; Agace, William LU and Quiding-Jarbrink, M
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lymphocyte trafficking, chemokine receptor, B cell, human, antibody-secreting cell
- in
- Clinical and Experimental Immunology
- volume
- 141
- issue
- 2
- pages
- 279 - 287
- publisher
- British Society for Immunology
- external identifiers
-
- wos:000230290900011
- pmid:15996192
- scopus:22344456234
- pmid:15996192
- ISSN
- 0009-9104
- DOI
- 10.1111/j.1365-2249.2005.02843.x
- language
- English
- LU publication?
- yes
- id
- ed901b41-25b6-4212-a51f-3c063fcabb65 (old id 233527)
- date added to LUP
- 2016-04-01 11:34:31
- date last changed
- 2022-02-03 01:33:43
@article{ed901b41-25b6-4212-a51f-3c063fcabb65, abstract = {{Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+ cells expressed CCR2, CCR3, or CCR9, and there was no difference in the expression of these receptors between IgA+ and IgG+ cells. In contrast, CCR4, CCR5, and CXCR3 was expressed on significantly more IgG+ than IgA+ cells. The function of chemokine receptors on memory B cells and ASC was then tested in the transwell system. IgG+ memory cells migrated to a higher extent than IgA+ cells towards the CXCR3 ligand CXCL11/I-TAC, while there was only a small migration towards the CCR4 ligand CCL17/TARC and the CCR9 ligand CCL25/TECK. ASC migrated poorly to all chemokines tested. In conclusion, this study shows that IgG+ and IgA+ memory B cells have a differential expression of the Th1 associated chemokine receptor CXCR3, as well as of CCR4 and CCR5. In contrast, none of the studied chemokine receptors was preferentially expressed by IgA+ cells.}}, author = {{Johansson, C and Ahlstedt, I and Furubacka, S and Johnsson, E and Agace, William and Quiding-Jarbrink, M}}, issn = {{0009-9104}}, keywords = {{lymphocyte trafficking; chemokine receptor; B cell; human; antibody-secreting cell}}, language = {{eng}}, number = {{2}}, pages = {{279--287}}, publisher = {{British Society for Immunology}}, series = {{Clinical and Experimental Immunology}}, title = {{Differential expression of chemokine receptors on human IgA plus and IgG plus B cells}}, url = {{http://dx.doi.org/10.1111/j.1365-2249.2005.02843.x}}, doi = {{10.1111/j.1365-2249.2005.02843.x}}, volume = {{141}}, year = {{2005}}, }