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Differential expression of chemokine receptors on human IgA plus and IgG plus B cells

Johansson, C; Ahlstedt, I; Furubacka, S; Johnsson, E; Agace, William LU and Quiding-Jarbrink, M (2005) In Clinical and Experimental Immunology 141(2). p.279-287
Abstract
Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+... (More)
Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+ cells expressed CCR2, CCR3, or CCR9, and there was no difference in the expression of these receptors between IgA+ and IgG+ cells. In contrast, CCR4, CCR5, and CXCR3 was expressed on significantly more IgG+ than IgA+ cells. The function of chemokine receptors on memory B cells and ASC was then tested in the transwell system. IgG+ memory cells migrated to a higher extent than IgA+ cells towards the CXCR3 ligand CXCL11/I-TAC, while there was only a small migration towards the CCR4 ligand CCL17/TARC and the CCR9 ligand CCL25/TECK. ASC migrated poorly to all chemokines tested. In conclusion, this study shows that IgG+ and IgA+ memory B cells have a differential expression of the Th1 associated chemokine receptor CXCR3, as well as of CCR4 and CCR5. In contrast, none of the studied chemokine receptors was preferentially expressed by IgA+ cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lymphocyte trafficking, chemokine receptor, B cell, human, antibody-secreting cell
in
Clinical and Experimental Immunology
volume
141
issue
2
pages
279 - 287
publisher
British Society for Immunology
external identifiers
  • wos:000230290900011
  • pmid:15996192
  • scopus:22344456234
ISSN
0009-9104
DOI
10.1111/j.1365-2249.2005.02843.x
language
English
LU publication?
yes
id
ed901b41-25b6-4212-a51f-3c063fcabb65 (old id 233527)
date added to LUP
2007-08-08 16:07:05
date last changed
2017-09-24 03:27:33
@article{ed901b41-25b6-4212-a51f-3c063fcabb65,
  abstract     = {Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+ cells expressed CCR2, CCR3, or CCR9, and there was no difference in the expression of these receptors between IgA+ and IgG+ cells. In contrast, CCR4, CCR5, and CXCR3 was expressed on significantly more IgG+ than IgA+ cells. The function of chemokine receptors on memory B cells and ASC was then tested in the transwell system. IgG+ memory cells migrated to a higher extent than IgA+ cells towards the CXCR3 ligand CXCL11/I-TAC, while there was only a small migration towards the CCR4 ligand CCL17/TARC and the CCR9 ligand CCL25/TECK. ASC migrated poorly to all chemokines tested. In conclusion, this study shows that IgG+ and IgA+ memory B cells have a differential expression of the Th1 associated chemokine receptor CXCR3, as well as of CCR4 and CCR5. In contrast, none of the studied chemokine receptors was preferentially expressed by IgA+ cells.},
  author       = {Johansson, C and Ahlstedt, I and Furubacka, S and Johnsson, E and Agace, William and Quiding-Jarbrink, M},
  issn         = {0009-9104},
  keyword      = {lymphocyte trafficking,chemokine receptor,B cell,human,antibody-secreting cell},
  language     = {eng},
  number       = {2},
  pages        = {279--287},
  publisher    = {British Society for Immunology},
  series       = {Clinical and Experimental Immunology},
  title        = {Differential expression of chemokine receptors on human IgA plus and IgG plus B cells},
  url          = {http://dx.doi.org/10.1111/j.1365-2249.2005.02843.x},
  volume       = {141},
  year         = {2005},
}