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Gel-forming and cell-associated mucins: preparation for structural and functional studies.

Davies, Julia; Wickström, Claes LU and Thornton, David J (2012) In Methods in Molecular Biology 842. p.27-47
Abstract
Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and... (More)
Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and high sugar content have been exploited for the separation of mucins from the majority of components in mucus secretions. In this chapter, we describe current and well-established methods (caesium chloride density-gradient centrifugation, gel-filtration and anion-exchange chromatography, and agarose gel electrophoresis) for the extraction and purification of gel-forming and cell-surface mucins which can subsequently be used for a variety of structural and functional studies. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
842
pages
27 - 47
publisher
Springer
external identifiers
  • pmid:22259128
  • scopus:84856347243
ISSN
1940-6029
DOI
10.1007/978-1-61779-513-8_2
language
English
LU publication?
yes
id
d6d4fe6d-4c15-4cc4-87dc-913100ceeed5 (old id 2336179)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22259128?dopt=Abstract
date added to LUP
2012-02-01 22:12:49
date last changed
2017-02-26 04:21:19
@article{d6d4fe6d-4c15-4cc4-87dc-913100ceeed5,
  abstract     = {Secreted and transmembrane mucins are important components of innate defence at the body's mucosal surfaces. The secreted mucins are large, polymeric glycoproteins, which are largely responsible for the gel-like properties of mucus secretions. The cell-tethered mucins, however, are monomeric but are typically composed of two subunits, a larger extracellular subunit which is heavily glycosylated while the smaller more sparsely glycosylated subunit has a short extracellular region, a single-pass transmembrane domain, and a cytoplasmic tail. These two families of mucins represent high-molecular-weight glycoproteins containing serine and threonine-rich domains that are the attachment sites for large numbers of O-glycans. The high-M ( r ) and high sugar content have been exploited for the separation of mucins from the majority of components in mucus secretions. In this chapter, we describe current and well-established methods (caesium chloride density-gradient centrifugation, gel-filtration and anion-exchange chromatography, and agarose gel electrophoresis) for the extraction and purification of gel-forming and cell-surface mucins which can subsequently be used for a variety of structural and functional studies.},
  author       = {Davies, Julia and Wickström, Claes and Thornton, David J},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {27--47},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Gel-forming and cell-associated mucins: preparation for structural and functional studies.},
  url          = {http://dx.doi.org/10.1007/978-1-61779-513-8_2},
  volume       = {842},
  year         = {2012},
}