Advanced

A method for rapid screening of ketone biotransformations: Detection of whole cell Baeyer-Villiger monooxygenase activity.

Linares-Pastén, Javier LU ; Chávez, Georgina LU ; Villagomez, Rodrigo; Mamo, Gashaw LU and Hatti-Kaul, Rajni LU (2012) In Enzyme and Microbial Technology 50(2). p.101-106
Abstract
A method for screening of ketone biotransformations was developed and applied to the identification of Baeyer-Villiger monooxygenase (BVMO) activity. The method was based on the formation of a purple coloured product on reaction between an enolizable ketone and 3,5-dinitrobenzoic acid in an alkaline solution. Absorbance of the colour decreased with the size of the cycloketone ring. Stoichiometric ratio between cycloketone and 3,5-dinitrobenzoic acid was 1:1 at maximum absorbance. The method was applied for monitoring the consumption of cyclohexanone by bacteria under aerobic conditions, and was found to be potentially useful for both screening assays and quantitative measurements of BVMO activity. Compared to other existing methods, this... (More)
A method for screening of ketone biotransformations was developed and applied to the identification of Baeyer-Villiger monooxygenase (BVMO) activity. The method was based on the formation of a purple coloured product on reaction between an enolizable ketone and 3,5-dinitrobenzoic acid in an alkaline solution. Absorbance of the colour decreased with the size of the cycloketone ring. Stoichiometric ratio between cycloketone and 3,5-dinitrobenzoic acid was 1:1 at maximum absorbance. The method was applied for monitoring the consumption of cyclohexanone by bacteria under aerobic conditions, and was found to be potentially useful for both screening assays and quantitative measurements of BVMO activity. Compared to other existing methods, this method is faster, cheaper and amenable for whole cell assays. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Enzyme and Microbial Technology
volume
50
issue
2
pages
101 - 106
publisher
Elsevier
external identifiers
  • wos:000300253900003
  • pmid:22226195
  • scopus:84855444200
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2011.10.004
language
English
LU publication?
yes
id
9304060d-38cd-437f-bd7b-56445197b712 (old id 2336607)
date added to LUP
2012-02-08 11:24:00
date last changed
2017-01-01 04:14:07
@article{9304060d-38cd-437f-bd7b-56445197b712,
  abstract     = {A method for screening of ketone biotransformations was developed and applied to the identification of Baeyer-Villiger monooxygenase (BVMO) activity. The method was based on the formation of a purple coloured product on reaction between an enolizable ketone and 3,5-dinitrobenzoic acid in an alkaline solution. Absorbance of the colour decreased with the size of the cycloketone ring. Stoichiometric ratio between cycloketone and 3,5-dinitrobenzoic acid was 1:1 at maximum absorbance. The method was applied for monitoring the consumption of cyclohexanone by bacteria under aerobic conditions, and was found to be potentially useful for both screening assays and quantitative measurements of BVMO activity. Compared to other existing methods, this method is faster, cheaper and amenable for whole cell assays.},
  author       = {Linares-Pastén, Javier and Chávez, Georgina and Villagomez, Rodrigo and Mamo, Gashaw and Hatti-Kaul, Rajni},
  issn         = {0141-0229},
  language     = {eng},
  number       = {2},
  pages        = {101--106},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {A method for rapid screening of ketone biotransformations: Detection of whole cell Baeyer-Villiger monooxygenase activity.},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2011.10.004},
  volume       = {50},
  year         = {2012},
}