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Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion - Interaction with the sensory system.

Csati, Anett; Tajti, Janos; Tuka, Bernadett; Edvinsson, Lars LU and Warfvinge, Karin LU (2012) In Brain Research 1435. p.29-39
Abstract
Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images... (More)
Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images were obtained using a light- and epifluorescence microscope coupled to a camera to visualize co-labeling by superimposing the digital images. In addition, Western blot technique was used to demonstrate the existence of CGRP receptor components in rat SPG. CGRP immunoreactive fibers were frequently found intraganglionic in the SPG in the vicinity of neurons. CLR immunoreactivity was observed in satellite glial cells (SGCs) as well as in nerve fibers, but not in neurons. RAMP1 immunoreactivity was localized in many neurons and SGCs. Thus, the two CGRP receptor components together were found in the SGCs. In addition, Western blot revealed the presence of RAMP1 and CLR in rat SPG. Our results suggest a possible sensory influence in the parasympathetic cranial ganglia. The sensory CGRP-containing fibers probably originate in the trigeminal ganglion, project to the SPG and act on CGRP receptors on SGCs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Brain Research
volume
1435
pages
29 - 39
publisher
Elsevier
external identifiers
  • wos:000301318400005
  • pmid:22208649
  • scopus:84856408156
ISSN
1872-6240
DOI
10.1016/j.brainres.2011.11.058
language
English
LU publication?
yes
id
4cd3e9de-8a76-42c0-a544-7191a009db9f (old id 2336820)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22208649?dopt=Abstract
date added to LUP
2012-02-01 18:48:27
date last changed
2017-11-19 04:17:49
@article{4cd3e9de-8a76-42c0-a544-7191a009db9f,
  abstract     = {Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images were obtained using a light- and epifluorescence microscope coupled to a camera to visualize co-labeling by superimposing the digital images. In addition, Western blot technique was used to demonstrate the existence of CGRP receptor components in rat SPG. CGRP immunoreactive fibers were frequently found intraganglionic in the SPG in the vicinity of neurons. CLR immunoreactivity was observed in satellite glial cells (SGCs) as well as in nerve fibers, but not in neurons. RAMP1 immunoreactivity was localized in many neurons and SGCs. Thus, the two CGRP receptor components together were found in the SGCs. In addition, Western blot revealed the presence of RAMP1 and CLR in rat SPG. Our results suggest a possible sensory influence in the parasympathetic cranial ganglia. The sensory CGRP-containing fibers probably originate in the trigeminal ganglion, project to the SPG and act on CGRP receptors on SGCs.},
  author       = {Csati, Anett and Tajti, Janos and Tuka, Bernadett and Edvinsson, Lars and Warfvinge, Karin},
  issn         = {1872-6240},
  language     = {eng},
  pages        = {29--39},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion - Interaction with the sensory system.},
  url          = {http://dx.doi.org/10.1016/j.brainres.2011.11.058},
  volume       = {1435},
  year         = {2012},
}