Proteome analysis of B-cell maturation.
Salonen, Johanna M ; Valmu, Leena ; Rönnholm, Gunilla ; Kalkkinen, Nisse and Vihinen, Mauno LU
(2006)
In Proteomics
6(19).
p.5152-5168
- Abstract
- Proteins affected by anti-mIgM stimulation during B-cell maturation were identified using 2-DE-based proteomics. We investigated the proteome profiles of stimulated and nonstimulated Ramos B-cells at eight time points during 5 d and compared the obtained proteomic data to the corresponding data from DNA-microarray studies. Anti-mIgM stimulation of the cells resulted in significant differences (> or =twofold) in the protein abundance close to 100 proteins and differences in post-translational protein modifications. Forty-eight up- or down-regulated proteins were identified by mass spectrometric methods and database searches. The identities of a further nine proteins were revealed by comparing their positions to the known proteins in... (More)
- Proteins affected by anti-mIgM stimulation during B-cell maturation were identified using 2-DE-based proteomics. We investigated the proteome profiles of stimulated and nonstimulated Ramos B-cells at eight time points during 5 d and compared the obtained proteomic data to the corresponding data from DNA-microarray studies. Anti-mIgM stimulation of the cells resulted in significant differences (> or =twofold) in the protein abundance close to 100 proteins and differences in post-translational protein modifications. Forty-eight up- or down-regulated proteins were identified by mass spectrometric methods and database searches. The identities of a further nine proteins were revealed by comparing their positions to the known proteins in other lymphocyte 2-DE databases. Several of the proteins are directly related to the functional and morphological characteristics of B-cells, such as cytoskeleton rearrangement and intracellular signalling triggered by the crosslinking of B-cell receptors. In addition to proteins known to be involved in human B-cell maturation, we identified several proteins that were not previously linked to lymphocyte differentiation. The results provide deeper insights into the process of B-cell maturation and may lead to novel therapeutic strategies for immunodeficiencies. An interactive 2-DE reference map is available at http://bioinf.uta.fi/BcellProteome. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3635356
- author
- Salonen, Johanna M
; Valmu, Leena
; Rönnholm, Gunilla
; Kalkkinen, Nisse
and Vihinen, Mauno
LU
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- B-Lymphocytes: chemistry, B-Lymphocytes: cytology, B-Lymphocytes: metabolism, Cell Differentiation: physiology, Down-Regulation: physiology, Immunoglobulin M: physiology, Proteome: genetics, Up-Regulation: physiology
- in
- Proteomics
- volume
- 6
- issue
- 19
- pages
- 5152 - 5168
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:16947121
- scopus:33749622765
- pmid:16947121
- ISSN
- 1615-9861
- DOI
- 10.1002/pmic.200600156
- language
- English
- LU publication?
- no
- id
- 23450a32-43f6-4588-bc03-369fcdcbca11 (old id 3635356)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/16947121?dopt=Abstract
- date added to LUP
- 2016-04-04 08:35:52
- date last changed
- 2022-01-29 03:42:53
@article{23450a32-43f6-4588-bc03-369fcdcbca11,
abstract = {{Proteins affected by anti-mIgM stimulation during B-cell maturation were identified using 2-DE-based proteomics. We investigated the proteome profiles of stimulated and nonstimulated Ramos B-cells at eight time points during 5 d and compared the obtained proteomic data to the corresponding data from DNA-microarray studies. Anti-mIgM stimulation of the cells resulted in significant differences (> or =twofold) in the protein abundance close to 100 proteins and differences in post-translational protein modifications. Forty-eight up- or down-regulated proteins were identified by mass spectrometric methods and database searches. The identities of a further nine proteins were revealed by comparing their positions to the known proteins in other lymphocyte 2-DE databases. Several of the proteins are directly related to the functional and morphological characteristics of B-cells, such as cytoskeleton rearrangement and intracellular signalling triggered by the crosslinking of B-cell receptors. In addition to proteins known to be involved in human B-cell maturation, we identified several proteins that were not previously linked to lymphocyte differentiation. The results provide deeper insights into the process of B-cell maturation and may lead to novel therapeutic strategies for immunodeficiencies. An interactive 2-DE reference map is available at http://bioinf.uta.fi/BcellProteome.}},
author = {{Salonen, Johanna M and Valmu, Leena and Rönnholm, Gunilla and Kalkkinen, Nisse and Vihinen, Mauno}},
issn = {{1615-9861}},
keywords = {{B-Lymphocytes: chemistry; B-Lymphocytes: cytology; B-Lymphocytes: metabolism; Cell Differentiation: physiology; Down-Regulation: physiology; Immunoglobulin M: physiology; Proteome: genetics; Up-Regulation: physiology}},
language = {{eng}},
number = {{19}},
pages = {{5152--5168}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Proteomics}},
title = {{Proteome analysis of B-cell maturation.}},
url = {{http://dx.doi.org/10.1002/pmic.200600156}},
doi = {{10.1002/pmic.200600156}},
volume = {{6}},
year = {{2006}},
}