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Overexpression of Aurora-A promotes laryngeal cancer progression by enhancing invasive ability and chromosomal instability

Zhang, Hao; Chen, Xuehua; Jin, Yuesheng LU ; Liu, Bingya and Zhou, Liang (2012) In European Archives of Oto-Rhino-Laryngology 269(2). p.607-614
Abstract
The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that... (More)
The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that expression of Aurora-A mRNA was significantly upregulated in laryngeal tumor tissue compared with that in normal tissue (P = 0.001), and overexpression of Aurora-A was found in 64.0% (16 of 25) of the patients by Western blotting. Upregulation of Aurora-A mRNA was significantly correlated with regional lymph node metastasis (P = 0.007) and clinical stage III/IV (P = 0.022). Overexpression of Aurora-A was significantly associated with lymph node metastasis (P = 0.027). Furthermore, disruption of Aurora-A using RNA interference technique suppressed invasive ability and chromosomal instability in HEp-2 cells. In conclusion, Aurora-A expression is elevated in human LSCC and associated with regional lymph node metastasis and late clinical stage. Overexpression of Aurora-A may contribute to LSCC carcinogenesis and progression partially due to enhancement of invasion ability and chromosomal instability. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aurora kinase, Laryngeal neoplasms, Cell invasion, Chromosomal, instability
in
European Archives of Oto-Rhino-Laryngology
volume
269
issue
2
pages
607 - 614
publisher
Springer
external identifiers
  • wos:000299174100035
  • scopus:84857064368
ISSN
0937-4477
DOI
10.1007/s00405-011-1629-4
language
English
LU publication?
yes
id
328d2a70-a4b5-44f3-901d-ddb0718910a9 (old id 2348805)
date added to LUP
2012-03-01 11:24:00
date last changed
2017-03-12 03:21:39
@article{328d2a70-a4b5-44f3-901d-ddb0718910a9,
  abstract     = {The purpose of the study was to investigate the expression of Aurora-A in human laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 cells. The expression of Aurora-A mRNA and protein were studied using reverse transcription-PCR and Western blot in LSCC tissues and corresponding normal epithelium, respectively. In addition, the correlation between Aurora-A expression and clinicopathologic characteristics was analyzed in LSCC patients. Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the effects of knockdown of Aurora-A on tumor invasion and chromosomal instability were investigated. The results showed that expression of Aurora-A mRNA was significantly upregulated in laryngeal tumor tissue compared with that in normal tissue (P = 0.001), and overexpression of Aurora-A was found in 64.0% (16 of 25) of the patients by Western blotting. Upregulation of Aurora-A mRNA was significantly correlated with regional lymph node metastasis (P = 0.007) and clinical stage III/IV (P = 0.022). Overexpression of Aurora-A was significantly associated with lymph node metastasis (P = 0.027). Furthermore, disruption of Aurora-A using RNA interference technique suppressed invasive ability and chromosomal instability in HEp-2 cells. In conclusion, Aurora-A expression is elevated in human LSCC and associated with regional lymph node metastasis and late clinical stage. Overexpression of Aurora-A may contribute to LSCC carcinogenesis and progression partially due to enhancement of invasion ability and chromosomal instability.},
  author       = {Zhang, Hao and Chen, Xuehua and Jin, Yuesheng and Liu, Bingya and Zhou, Liang},
  issn         = {0937-4477},
  keyword      = {Aurora kinase,Laryngeal neoplasms,Cell invasion,Chromosomal,instability},
  language     = {eng},
  number       = {2},
  pages        = {607--614},
  publisher    = {Springer},
  series       = {European Archives of Oto-Rhino-Laryngology},
  title        = {Overexpression of Aurora-A promotes laryngeal cancer progression by enhancing invasive ability and chromosomal instability},
  url          = {http://dx.doi.org/10.1007/s00405-011-1629-4},
  volume       = {269},
  year         = {2012},
}