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Immunoregulatory cytokines modify Escherichia coli induced uroepithelial cell IL-6 and IL-8 responses

Hedges, Spencer R. ; Bjarnadottir, Maria LU ; Agace, William LU ; Hang, Long LU and Svanborg, Catharina LU (1996) In Cytokine 8(9). p.686-697
Abstract

This study analysed the effects of immunoregulatory cytokines on uroepithelial cell cytokine responses. The A-498 human kidney cell line was treated with the interleukins IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, interferon gamma (IFN-α) and transforming growth factor beta (TGF-β1). Secreted IL-6 and IL-8 were quantitated by enzyme-linked immunoabsorbent assay (ELISA) and bioassay; IL-6 and IL-8 mRNA species were quantitated by reverse transcriptase polymerase chain reaction (RT-PCR). IL-4, IL-13, IFN-γ, and TGF-β1, but not IL-2, IL-5, IL-10 or IL-12, stimulated IL-6 secretion. At high concentrations, IL-4 and IL-13 stimulated low levels of IL-8 secretion. Immunoregulatory cytokines were analysed for their ability to modify the A-498... (More)

This study analysed the effects of immunoregulatory cytokines on uroepithelial cell cytokine responses. The A-498 human kidney cell line was treated with the interleukins IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, interferon gamma (IFN-α) and transforming growth factor beta (TGF-β1). Secreted IL-6 and IL-8 were quantitated by enzyme-linked immunoabsorbent assay (ELISA) and bioassay; IL-6 and IL-8 mRNA species were quantitated by reverse transcriptase polymerase chain reaction (RT-PCR). IL-4, IL-13, IFN-γ, and TGF-β1, but not IL-2, IL-5, IL-10 or IL-12, stimulated IL-6 secretion. At high concentrations, IL-4 and IL-13 stimulated low levels of IL-8 secretion. Immunoregulatory cytokines were analysed for their ability to modify the A-498 cells' IL-6 and IL-8 secretion in response to Escherichia coli. IL-5, IL-12, IL-13 and TGF-β1 additively enhanced the bacterially induced IL-6 secretion, but they did not affect IL-8 secretion. The strongest affects on uroepithelial cell IL-6 and IL-8 responses in the presence of bacteria were observed in conjunction with IL-4 and IFN-α. IL-4 induced IL-6 production in synergy with E. coli. IFN-α both enhanced and inhibited IL-6 and IL-8 responses in combination with E. coli, depending on the order of stimulant addition. The results demonstrate that immunoregulatory cytokines can modify the uroepithelial cell responses to bacteria in vitro. In this way T cells may regulate the cytokine responses of uroepithelial and possibly other mucosal epithelial cells in vivo.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Escherichia coli, IL-6 and IL-8 responses, RT-PCR
in
Cytokine
volume
8
issue
9
pages
12 pages
publisher
Academic Press
external identifiers
  • pmid:8932979
  • scopus:0030250030
ISSN
1043-4666
DOI
10.1006/cyto.1996.0091
language
English
LU publication?
yes
id
234ab6d5-74d8-476e-8b43-2ee6905414d7
date added to LUP
2019-05-30 13:52:30
date last changed
2024-04-02 07:54:28
@article{234ab6d5-74d8-476e-8b43-2ee6905414d7,
  abstract     = {{<p>This study analysed the effects of immunoregulatory cytokines on uroepithelial cell cytokine responses. The A-498 human kidney cell line was treated with the interleukins IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, interferon gamma (IFN-α) and transforming growth factor beta (TGF-β1). Secreted IL-6 and IL-8 were quantitated by enzyme-linked immunoabsorbent assay (ELISA) and bioassay; IL-6 and IL-8 mRNA species were quantitated by reverse transcriptase polymerase chain reaction (RT-PCR). IL-4, IL-13, IFN-γ, and TGF-β1, but not IL-2, IL-5, IL-10 or IL-12, stimulated IL-6 secretion. At high concentrations, IL-4 and IL-13 stimulated low levels of IL-8 secretion. Immunoregulatory cytokines were analysed for their ability to modify the A-498 cells' IL-6 and IL-8 secretion in response to Escherichia coli. IL-5, IL-12, IL-13 and TGF-β1 additively enhanced the bacterially induced IL-6 secretion, but they did not affect IL-8 secretion. The strongest affects on uroepithelial cell IL-6 and IL-8 responses in the presence of bacteria were observed in conjunction with IL-4 and IFN-α. IL-4 induced IL-6 production in synergy with E. coli. IFN-α both enhanced and inhibited IL-6 and IL-8 responses in combination with E. coli, depending on the order of stimulant addition. The results demonstrate that immunoregulatory cytokines can modify the uroepithelial cell responses to bacteria in vitro. In this way T cells may regulate the cytokine responses of uroepithelial and possibly other mucosal epithelial cells in vivo.</p>}},
  author       = {{Hedges, Spencer R. and Bjarnadottir, Maria and Agace, William and Hang, Long and Svanborg, Catharina}},
  issn         = {{1043-4666}},
  keywords     = {{Escherichia coli; IL-6 and IL-8 responses; RT-PCR}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{9}},
  pages        = {{686--697}},
  publisher    = {{Academic Press}},
  series       = {{Cytokine}},
  title        = {{Immunoregulatory cytokines modify Escherichia coli induced uroepithelial cell IL-6 and IL-8 responses}},
  url          = {{http://dx.doi.org/10.1006/cyto.1996.0091}},
  doi          = {{10.1006/cyto.1996.0091}},
  volume       = {{8}},
  year         = {{1996}},
}