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Large Scale Biobanking of Blood – The Importance of High Density Sample Processing Procedures

Malm, Johan LU ; Végvári, Ákos LU ; Rezeli, Melinda LU ; Upton, Paul; Danmyr, Pia LU ; Nilsson, Rolf; Steinfelder, Erik and Marko-Varga, György LU (2012) In Journal of Proteomics 76(1). p.116-124
Abstract
We introduce a novel automated sample-processing concept that will be of mandatory importance to proteomics and future clinical research, performing patient studies from resulting blood fractions in various disease areas. Biobank storage of small sample volumes allows for high replicate numbers to be processed and aliquoted, where each sample aliquot can be used for a dedicated clinical analysis and end-point measurement. In order to preserve sample integrity and value over time, the principle of single usage is gaining recognition. We hereby present a 384-format sample tube system for the preservation and archiving of clinical patient samples that will form the basis for future proteomics studies. This high density scaling allows for... (More)
We introduce a novel automated sample-processing concept that will be of mandatory importance to proteomics and future clinical research, performing patient studies from resulting blood fractions in various disease areas. Biobank storage of small sample volumes allows for high replicate numbers to be processed and aliquoted, where each sample aliquot can be used for a dedicated clinical analysis and end-point measurement. In order to preserve sample integrity and value over time, the principle of single usage is gaining recognition. We hereby present a 384-format sample tube system for the preservation and archiving of clinical patient samples that will form the basis for future proteomics studies. This high density scaling allows for reproducible aliquoting 70-µL volumes of blood fractions. Blood plasma with EDTA, Li-heparin, and citrate, as anti-coagulants, are fractioned along with the buffy coat and the erythrocyte fraction, in addition to the serum fraction. We demonstrate an automated sample handling for biobanking: samples from patients were processed and aliquoted in both 96- and 384-sample racks by liquid handling robotics and Laboratory Intelligence Management System (LIMS) overview and control. Within this study, the blood samples were analyzed by the Clinical Chemistry department at the Southern University Hospital in Malmö, using standard biomarker assays, quantifying 23 common markers used in everyday healthcare around the world. We were able to prove that the 384-format using an aluminum foil with a thin polymer film coating for sealing, is stable and can reproducibly be processed for automated biobank freezer units. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteomics
volume
76
issue
1
pages
116 - 124
publisher
Elsevier
external identifiers
  • wos:000313391100012
  • scopus:84869861248
ISSN
1874-3919
DOI
10.1016/j.jprot.2012.05.003
language
English
LU publication?
yes
id
f2eae3dd-ffbb-4448-81a8-7ea6d7222468 (old id 2364971)
date added to LUP
2012-05-09 14:44:41
date last changed
2017-04-09 03:17:51
@article{f2eae3dd-ffbb-4448-81a8-7ea6d7222468,
  abstract     = {We introduce a novel automated sample-processing concept that will be of mandatory importance to proteomics and future clinical research, performing patient studies from resulting blood fractions in various disease areas. Biobank storage of small sample volumes allows for high replicate numbers to be processed and aliquoted, where each sample aliquot can be used for a dedicated clinical analysis and end-point measurement. In order to preserve sample integrity and value over time, the principle of single usage is gaining recognition. We hereby present a 384-format sample tube system for the preservation and archiving of clinical patient samples that will form the basis for future proteomics studies. This high density scaling allows for reproducible aliquoting 70-µL volumes of blood fractions. Blood plasma with EDTA, Li-heparin, and citrate, as anti-coagulants, are fractioned along with the buffy coat and the erythrocyte fraction, in addition to the serum fraction. We demonstrate an automated sample handling for biobanking: samples from patients were processed and aliquoted in both 96- and 384-sample racks by liquid handling robotics and Laboratory Intelligence Management System (LIMS) overview and control. Within this study, the blood samples were analyzed by the Clinical Chemistry department at the Southern University Hospital in Malmö, using standard biomarker assays, quantifying 23 common markers used in everyday healthcare around the world. We were able to prove that the 384-format using an aluminum foil with a thin polymer film coating for sealing, is stable and can reproducibly be processed for automated biobank freezer units.},
  author       = {Malm, Johan and Végvári, Ákos and Rezeli, Melinda and Upton, Paul and Danmyr, Pia and Nilsson, Rolf and Steinfelder, Erik and Marko-Varga, György},
  issn         = {1874-3919},
  language     = {eng},
  number       = {1},
  pages        = {116--124},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics},
  title        = {Large Scale Biobanking of Blood – The Importance of High Density Sample Processing Procedures},
  url          = {http://dx.doi.org/10.1016/j.jprot.2012.05.003},
  volume       = {76},
  year         = {2012},
}