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Surface microdialysis sampling: a new approach described in a liver ischaemia model.

Abrahamsson, Pernilla; Aberg, Anna-Maja; Winsö, Ola; Johansson, Göran; Haney, Michael and Blind, Per-Jonas LU (2012) In Clinical Physiology and Functional Imaging 32(2). p.99-105
Abstract
We recently have shown that samples from microdialysis (MD) probes placed on the surface of the heart reflect metabolic events in the myocardium. This new interesting observation challenges us to consider whether surface application of MD applies to other parenchymatous organs and their surfaces. In 13 anesthetized pigs, transient liver ischaemia was achieved by occlusion of arterial and venous inflow to the liver. Two probes on liver surface and two in parenchyma were perfused with a flow rate of 1 μl per min (n = 13). An identical set-up was used for probes with a flow rate of 2 μl per min (n = 9). Samples were collected for every 15-min period during 60 min of baseline, 45 min of ischaemia and 60 min of reperfusion. Lactate, glucose,... (More)
We recently have shown that samples from microdialysis (MD) probes placed on the surface of the heart reflect metabolic events in the myocardium. This new interesting observation challenges us to consider whether surface application of MD applies to other parenchymatous organs and their surfaces. In 13 anesthetized pigs, transient liver ischaemia was achieved by occlusion of arterial and venous inflow to the liver. Two probes on liver surface and two in parenchyma were perfused with a flow rate of 1 μl per min (n = 13). An identical set-up was used for probes with a flow rate of 2 μl per min (n = 9). Samples were collected for every 15-min period during 60 min of baseline, 45 min of ischaemia and 60 min of reperfusion. Lactate, glucose, pyruvate and glycerol were analysed in MD samples. We focused on relative changes in the present study. There was a strong agreement in relative lactate and glucose levels between probes placed on liver surface and those on parenchyma. No significant differences in relative changes in lactate and glucose levels were seen between samples from surface probes and probes in liver parenchyma during equilibration, baseline, ischaemia or reperfusion with a flow rate of 1 μl per min. MD sampling applied on the liver surface is a new application area for the MD technique and may be used to monitor liver metabolism during both physiological and pathophysiological conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Physiology and Functional Imaging
volume
32
issue
2
pages
99 - 105
publisher
Wiley Online Library
external identifiers
  • wos:000299734400004
  • pmid:22296629
  • scopus:84856442807
ISSN
1475-0961
DOI
10.1111/j.1475-097X.2011.01061.x
language
English
LU publication?
yes
id
42792cb6-4f56-4f8f-a065-b0a3c84c1b57 (old id 2367464)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22296629?dopt=Abstract
date added to LUP
2012-03-02 08:21:18
date last changed
2017-04-30 15:20:49
@article{42792cb6-4f56-4f8f-a065-b0a3c84c1b57,
  abstract     = {We recently have shown that samples from microdialysis (MD) probes placed on the surface of the heart reflect metabolic events in the myocardium. This new interesting observation challenges us to consider whether surface application of MD applies to other parenchymatous organs and their surfaces. In 13 anesthetized pigs, transient liver ischaemia was achieved by occlusion of arterial and venous inflow to the liver. Two probes on liver surface and two in parenchyma were perfused with a flow rate of 1 μl per min (n = 13). An identical set-up was used for probes with a flow rate of 2 μl per min (n = 9). Samples were collected for every 15-min period during 60 min of baseline, 45 min of ischaemia and 60 min of reperfusion. Lactate, glucose, pyruvate and glycerol were analysed in MD samples. We focused on relative changes in the present study. There was a strong agreement in relative lactate and glucose levels between probes placed on liver surface and those on parenchyma. No significant differences in relative changes in lactate and glucose levels were seen between samples from surface probes and probes in liver parenchyma during equilibration, baseline, ischaemia or reperfusion with a flow rate of 1 μl per min. MD sampling applied on the liver surface is a new application area for the MD technique and may be used to monitor liver metabolism during both physiological and pathophysiological conditions.},
  author       = {Abrahamsson, Pernilla and Aberg, Anna-Maja and Winsö, Ola and Johansson, Göran and Haney, Michael and Blind, Per-Jonas},
  issn         = {1475-0961},
  language     = {eng},
  number       = {2},
  pages        = {99--105},
  publisher    = {Wiley Online Library},
  series       = {Clinical Physiology and Functional Imaging},
  title        = {Surface microdialysis sampling: a new approach described in a liver ischaemia model.},
  url          = {http://dx.doi.org/10.1111/j.1475-097X.2011.01061.x},
  volume       = {32},
  year         = {2012},
}