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Quality control workshops in standardization of sperm concentration and motility assessment in multicentre studies

Toft, G; Rignell-Hydbom, Anna LU ; Tyrkiel, E; Shvets, N and Giwercman, Aleksander LU (2005) In International Journal of Andrology 28(3). p.144-149
Abstract
Semen quality has been reported to vary markedly between different regions. To properly assess the differences among countries a minimization of the variation among centres in the assessments of sperm quality is essential. We here report on the training and two subsequent follow-up workshops on assessments of sperm concentration and motility. A total of 26 fresh ejaculates were analysed by four persons who were collecting these data in a multicentre study on sperm quality. In addition, two trained technicians from one of the laboratories analysed the samples. At the first training workshop, the median coefficient of variation among centres in sperm concentration was 27.7%, but decreased markedly to 17.0% at the second workshop and 8.1% (P... (More)
Semen quality has been reported to vary markedly between different regions. To properly assess the differences among countries a minimization of the variation among centres in the assessments of sperm quality is essential. We here report on the training and two subsequent follow-up workshops on assessments of sperm concentration and motility. A total of 26 fresh ejaculates were analysed by four persons who were collecting these data in a multicentre study on sperm quality. In addition, two trained technicians from one of the laboratories analysed the samples. At the first training workshop, the median coefficient of variation among centres in sperm concentration was 27.7%, but decreased markedly to 17.0% at the second workshop and 8.1% (P = 0.048) at the third workshop. The CV for evaluation of the proportion of progressive motile sperm decreased less and not statistically significant from 16.5 to 14.1% and 11.0% (P = 0.94). At the third workshop there were no statistical significant differences among centres in assessments of sperm concentration or motility (p > 0.57). Furthermore the coefficient of variation in assessments of sperm concentration and motility among centres were at the same level as between two trained technicians (p > 0.72). This study indicates that training and subsequent follow-up workshops can assure minimum variability among centres in assessments of sperm concentration and motility. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
motility, variance, sperm concentration, multicentre studies, quality control, sperm
in
International Journal of Andrology
volume
28
issue
3
pages
144 - 149
publisher
Wiley-Blackwell
external identifiers
  • pmid:15910539
  • wos:000229583800004
  • scopus:20344375959
ISSN
0105-6263
DOI
10.1111/j.1365-2605.2005.00518.x
language
English
LU publication?
yes
id
7e65722d-96dc-4af6-b455-40b4888f88e3 (old id 237472)
date added to LUP
2007-08-23 09:41:33
date last changed
2017-01-01 06:44:13
@article{7e65722d-96dc-4af6-b455-40b4888f88e3,
  abstract     = {Semen quality has been reported to vary markedly between different regions. To properly assess the differences among countries a minimization of the variation among centres in the assessments of sperm quality is essential. We here report on the training and two subsequent follow-up workshops on assessments of sperm concentration and motility. A total of 26 fresh ejaculates were analysed by four persons who were collecting these data in a multicentre study on sperm quality. In addition, two trained technicians from one of the laboratories analysed the samples. At the first training workshop, the median coefficient of variation among centres in sperm concentration was 27.7%, but decreased markedly to 17.0% at the second workshop and 8.1% (P = 0.048) at the third workshop. The CV for evaluation of the proportion of progressive motile sperm decreased less and not statistically significant from 16.5 to 14.1% and 11.0% (P = 0.94). At the third workshop there were no statistical significant differences among centres in assessments of sperm concentration or motility (p > 0.57). Furthermore the coefficient of variation in assessments of sperm concentration and motility among centres were at the same level as between two trained technicians (p > 0.72). This study indicates that training and subsequent follow-up workshops can assure minimum variability among centres in assessments of sperm concentration and motility.},
  author       = {Toft, G and Rignell-Hydbom, Anna and Tyrkiel, E and Shvets, N and Giwercman, Aleksander},
  issn         = {0105-6263},
  keyword      = {motility,variance,sperm concentration,multicentre studies,quality control,sperm},
  language     = {eng},
  number       = {3},
  pages        = {144--149},
  publisher    = {Wiley-Blackwell},
  series       = {International Journal of Andrology},
  title        = {Quality control workshops in standardization of sperm concentration and motility assessment in multicentre studies},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2005.00518.x},
  volume       = {28},
  year         = {2005},
}