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CD40 employs p38 MAP kinase in IgE isotype switching

Brady, K.; Fitzgerald, S.; Ingvarsson, Sigurdur LU ; Borrebaeck, Carl LU and Moynagh, P. N. (2001) In Biochemical and Biophysical Research Communications 289(1). p.276-281
Abstract
IgE switching requires the prior induction of CE germline transcripts which is mediated by the concerted binding of STAT-6 and NF kappaB to the CE promoter. These transcription factors are regulated by IL-4 and CD40, respectively. However the latter can effect other signaling pathways and the present study explores the role of p38 MAPK in induction of CE germline transcripts. CD40 and IL-4, both alone and in synergy, were initially shown to activate the CE promoter in a B cell lymphoma cell line. Under the same conditions CD40 caused activation of p38 MAPK, whereas IL-4 was ineffective. The p38 MAPK inhibitor, SB203580, and a dominant negative form of p38 MAPK decreased the CD40 activation of the CE promoter by reducing the ability of CD40... (More)
IgE switching requires the prior induction of CE germline transcripts which is mediated by the concerted binding of STAT-6 and NF kappaB to the CE promoter. These transcription factors are regulated by IL-4 and CD40, respectively. However the latter can effect other signaling pathways and the present study explores the role of p38 MAPK in induction of CE germline transcripts. CD40 and IL-4, both alone and in synergy, were initially shown to activate the CE promoter in a B cell lymphoma cell line. Under the same conditions CD40 caused activation of p38 MAPK, whereas IL-4 was ineffective. The p38 MAPK inhibitor, SB203580, and a dominant negative form of p38 MAPK decreased the CD40 activation of the CE promoter by reducing the ability of CD40 to increase the transactivation potential of NF kappaB. This study suggests that p38 MAPK is crucially important in mediating CD40 activation of NFKB which acts to induce CE germline transcripts, ultimately facilitating IgE switching. (C) 2001 Academic Press. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
CD40, IgE, isotype switching, allergy, p38 MAP kinase, NF kappa B, ACTIVATED PROTEIN-KINASE, TUMOR-NECROSIS-FACTOR, CROSS-LINKING CD40, FACTOR-KAPPA-B, GERMLINE PROMOTER, CELLS, TRANSCRIPTION, INTERLEUKIN-4, EXPRESSION, MICE
in
Biochemical and Biophysical Research Communications
volume
289
issue
1
pages
276 - 281
publisher
Elsevier
external identifiers
  • wos:000172451100046
  • scopus:0035941089
ISSN
1090-2104
DOI
10.1006/bbrc.2001.5968
language
English
LU publication?
yes
id
668628a4-1d50-41b2-8bfd-bcbd92e337cc (old id 2376275)
date added to LUP
2012-03-23 09:53:58
date last changed
2018-05-29 11:28:08
@article{668628a4-1d50-41b2-8bfd-bcbd92e337cc,
  abstract     = {IgE switching requires the prior induction of CE germline transcripts which is mediated by the concerted binding of STAT-6 and NF kappaB to the CE promoter. These transcription factors are regulated by IL-4 and CD40, respectively. However the latter can effect other signaling pathways and the present study explores the role of p38 MAPK in induction of CE germline transcripts. CD40 and IL-4, both alone and in synergy, were initially shown to activate the CE promoter in a B cell lymphoma cell line. Under the same conditions CD40 caused activation of p38 MAPK, whereas IL-4 was ineffective. The p38 MAPK inhibitor, SB203580, and a dominant negative form of p38 MAPK decreased the CD40 activation of the CE promoter by reducing the ability of CD40 to increase the transactivation potential of NF kappaB. This study suggests that p38 MAPK is crucially important in mediating CD40 activation of NFKB which acts to induce CE germline transcripts, ultimately facilitating IgE switching. (C) 2001 Academic Press.},
  author       = {Brady, K. and Fitzgerald, S. and Ingvarsson, Sigurdur and Borrebaeck, Carl and Moynagh, P. N.},
  issn         = {1090-2104},
  keyword      = {CD40,IgE,isotype switching,allergy,p38 MAP kinase,NF kappa B,ACTIVATED PROTEIN-KINASE,TUMOR-NECROSIS-FACTOR,CROSS-LINKING CD40,FACTOR-KAPPA-B,GERMLINE PROMOTER,CELLS,TRANSCRIPTION,INTERLEUKIN-4,EXPRESSION,MICE},
  language     = {eng},
  number       = {1},
  pages        = {276--281},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {CD40 employs p38 MAP kinase in IgE isotype switching},
  url          = {http://dx.doi.org/10.1006/bbrc.2001.5968},
  volume       = {289},
  year         = {2001},
}