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The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

Söderlind, Eskil LU ; Carlsson, Roland LU ; Borrebaeck, Carl LU and Ohlin, Mats LU (2001) In Combinatorial Chemistry & High Throughput Screening 4(5). p.409-416
Abstract
Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates... (More)
Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions. (Less)
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organization
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subject
keywords
COMPLEMENTARITY-DETERMINING REGIONS, PHAGE DISPLAY LIBRARY, SINGLE-CHAIN, FV, AFFINITY HUMAN-ANTIBODIES, BY-PASSING IMMUNIZATION, RANDOM, MUTAGENESIS, BINDING-SITE, V-H, SYNTHETIC REPERTOIRES, SOMATIC, HYPERMUTATION
in
Combinatorial Chemistry & High Throughput Screening
volume
4
issue
5
pages
409 - 416
publisher
Bentham Science Publishers
external identifiers
  • wos:000170235500005
  • scopus:0034869427
ISSN
1386-2073
language
English
LU publication?
yes
id
84d37d34-a8e2-473a-99b1-4a32be806190 (old id 2376451)
date added to LUP
2012-03-23 08:55:10
date last changed
2018-05-29 10:25:50
@article{84d37d34-a8e2-473a-99b1-4a32be806190,
  abstract     = {Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.},
  author       = {Söderlind, Eskil and Carlsson, Roland and Borrebaeck, Carl and Ohlin, Mats},
  issn         = {1386-2073},
  keyword      = {COMPLEMENTARITY-DETERMINING REGIONS,PHAGE DISPLAY LIBRARY,SINGLE-CHAIN,FV,AFFINITY HUMAN-ANTIBODIES,BY-PASSING IMMUNIZATION,RANDOM,MUTAGENESIS,BINDING-SITE,V-H,SYNTHETIC REPERTOIRES,SOMATIC,HYPERMUTATION},
  language     = {eng},
  number       = {5},
  pages        = {409--416},
  publisher    = {Bentham Science Publishers},
  series       = {Combinatorial Chemistry & High Throughput Screening},
  title        = {The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds},
  volume       = {4},
  year         = {2001},
}