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Novel glycolipid variations revealed by monoclonal antibody immunochemical analysis of weak ABO subgroups of A

Svensson, L; Rydberg, L; Hellberg, Åsa LU ; Gilliver, LG; Olsson, ML and Henry, SM (2005) In Vox Sanguinis 89(1). p.27-38
Abstract
Background and Objectives The chemical basis of the subgroups of A is largely unknown. We used thin-layer chromatography immunochemical staining techniques together with a range of characterized monoclonal reagents to analyse glycolipids isolated from a variety of weak subgroups. Materials and Methods Glycolipids isolated from red cells collected from nine genetically defined individuals of the rare subgroups of A, including a novel A(3) allele (A(2) 539G > A) not described previously, were subjected to a highly sensitive thin-layer chromatographic immunochemical analysis. Results Semicharacterized monoclonal antibodies revealed that, in addition to the expected quantitative differences between common phenotypes and the weak subgroups,... (More)
Background and Objectives The chemical basis of the subgroups of A is largely unknown. We used thin-layer chromatography immunochemical staining techniques together with a range of characterized monoclonal reagents to analyse glycolipids isolated from a variety of weak subgroups. Materials and Methods Glycolipids isolated from red cells collected from nine genetically defined individuals of the rare subgroups of A, including a novel A(3) allele (A(2) 539G > A) not described previously, were subjected to a highly sensitive thin-layer chromatographic immunochemical analysis. Results Semicharacterized monoclonal antibodies revealed that, in addition to the expected quantitative differences between common phenotypes and the weak subgroups, qualitative glycolipid differences (or at least an apparent qualitative basis), caused by major changes in the ratios of different structures exist. Specifically it was found that the weakest A-expressing samples (A(el) phenotype) appeared to express an unusual A structure in the 8-12 sugar region. Variable expression of several structures in one of the A weak samples were suggestive of novel blood group A structures. Conclusions Although no structural characterization could be undertaken, the results are clearly indicative that the variant glycosyltransferases of the rare ABO subgroups are not only inefficient, but they may potentially synthesize novel ABO structures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunochemistry, glycolipid, ABO, genotype, subgroups
in
Vox Sanguinis
volume
89
issue
1
pages
27 - 38
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000229491900004
  • pmid:15938737
  • scopus:20544456881
ISSN
1423-0410
DOI
10.1111/j.1423-0410.2005.00642.x
language
English
LU publication?
yes
id
2cc427c1-11ae-4cd5-98dd-51736ed1f82d (old id 237647)
date added to LUP
2007-08-20 14:17:31
date last changed
2017-10-01 04:44:11
@article{2cc427c1-11ae-4cd5-98dd-51736ed1f82d,
  abstract     = {Background and Objectives The chemical basis of the subgroups of A is largely unknown. We used thin-layer chromatography immunochemical staining techniques together with a range of characterized monoclonal reagents to analyse glycolipids isolated from a variety of weak subgroups. Materials and Methods Glycolipids isolated from red cells collected from nine genetically defined individuals of the rare subgroups of A, including a novel A(3) allele (A(2) 539G > A) not described previously, were subjected to a highly sensitive thin-layer chromatographic immunochemical analysis. Results Semicharacterized monoclonal antibodies revealed that, in addition to the expected quantitative differences between common phenotypes and the weak subgroups, qualitative glycolipid differences (or at least an apparent qualitative basis), caused by major changes in the ratios of different structures exist. Specifically it was found that the weakest A-expressing samples (A(el) phenotype) appeared to express an unusual A structure in the 8-12 sugar region. Variable expression of several structures in one of the A weak samples were suggestive of novel blood group A structures. Conclusions Although no structural characterization could be undertaken, the results are clearly indicative that the variant glycosyltransferases of the rare ABO subgroups are not only inefficient, but they may potentially synthesize novel ABO structures.},
  author       = {Svensson, L and Rydberg, L and Hellberg, Åsa and Gilliver, LG and Olsson, ML and Henry, SM},
  issn         = {1423-0410},
  keyword      = {immunochemistry,glycolipid,ABO,genotype,subgroups},
  language     = {eng},
  number       = {1},
  pages        = {27--38},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Vox Sanguinis},
  title        = {Novel glycolipid variations revealed by monoclonal antibody immunochemical analysis of weak ABO subgroups of A},
  url          = {http://dx.doi.org/10.1111/j.1423-0410.2005.00642.x},
  volume       = {89},
  year         = {2005},
}