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A novel membraneless β-glucan/O2 enzymatic fuel cell based on β-glucosidase (RmBgl3B)/pyranose dehydrogenase (AmPDH) co-immobilized onto buckypaper electrode

Rafighi, Parvin ; Nordberg Karlsson, Eva LU orcid ; Zubaida Gulshan Ara, Kazi ; Pankratova, Galina LU ; Bollella, Paolo ; Peterbauer, Clemens K. and Gorton, Lo LU (2022) In Bioelectrochemistry 148.
Abstract

A novel membraneless β-glucan/O2 enzymatic fuel cell was developed by combining a bioanode based on buckypaper modified with co-immobilized Agaricus meleagris pyranose dehydrogenase (AmPDH) and Rhodothermus marinus β-glucosidase (RmBgl3B) (RmBgl3B-AmPDH/buckypaper) with a biocathode based on solid graphite modified with Myrothecium verrucaria bilirubin oxidase (MvBOx/graphite). AmPDH was connected electrochemically with the buckypaper using an osmium redox polymer in a mediated reaction, whereas MvBOx was connected with graphite in a direct electron transfer reaction.

The fuel for the bioanode was produced by enzymatic hydrolysis... (More)

A novel membraneless β-glucan/O2 enzymatic fuel cell was developed by combining a bioanode based on buckypaper modified with co-immobilized Agaricus meleagris pyranose dehydrogenase (AmPDH) and Rhodothermus marinus β-glucosidase (RmBgl3B) (RmBgl3B-AmPDH/buckypaper) with a biocathode based on solid graphite modified with Myrothecium verrucaria bilirubin oxidase (MvBOx/graphite). AmPDH was connected electrochemically with the buckypaper using an osmium redox polymer in a mediated reaction, whereas MvBOx was connected with graphite in a direct electron transfer reaction.

The fuel for the bioanode was produced by enzymatic hydrolysis of β-glucan by the exoglucanase RmBgl3B into d-glucose, which in turn was enzymatically oxidised by AmPDH to generate a current response. This design allows to obtain an efficient enzymatic fuel cell, where the chemical energy converted into electrical energy is higher than the chemical energy stored in complex carbohydrate based fuel.

The maximum power density of the assembled β-glucan/O2 biofuel cell reached 26.3 ± 4.6 μWcm−2 at 0.36 V in phosphate buffer containing 0.5 % (w/v) β-glucan at 40 °C with excellent stability retaining 68.6 % of its initial performance after 5 days. The result confirms that β-glucan can be employed as fuel in an enzymatic biofuel cell.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Agaricales, Bioelectric Energy Sources, Electrodes, Enzymes, Immobilized, Glucose, Graphite, Osmium, Phosphates, Polymers, Rhodothermus, beta-Glucans, beta-Glucosidase
in
Bioelectrochemistry
volume
148
article number
108254
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85138027748
  • pmid:36122427
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2022.108254
language
English
LU publication?
yes
additional info
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
id
23775b4f-3eed-46bc-bc40-6e063ab43639
date added to LUP
2022-10-27 09:10:13
date last changed
2024-04-18 15:13:40
@article{23775b4f-3eed-46bc-bc40-6e063ab43639,
  abstract     = {{<p>A novel membraneless β-glucan/O<sub>2</sub> enzymatic fuel cell was developed by combining a bioanode based on buckypaper modified with co-immobilized <em>Agaricus meleagris</em> pyranose dehydrogenase (<em>Am</em>PDH) and <em>Rhodothermus marinus</em> β-glucosidase (<em>Rm</em>Bgl3B) (<em>Rm</em>Bgl3B-<em>Am</em>PDH/buckypaper) with a biocathode based on solid graphite modified with <em>Myrothecium verrucaria</em> bilirubin oxidase (<em>Mv</em>BOx/graphite). <em>Am</em>PDH was connected electrochemically with the buckypaper using an osmium redox polymer in a mediated reaction, whereas <em>Mv</em>BOx was connected with graphite in a direct electron transfer reaction.</p><p>The fuel for the bioanode was produced by enzymatic hydrolysis of β-glucan by the exoglucanase <em>Rm</em>Bgl3B into d-glucose, which in turn was enzymatically oxidised by <em>Am</em>PDH to generate a current response. This design allows to obtain an efficient enzymatic fuel cell, where the chemical energy converted into electrical energy is higher than the chemical energy stored in complex carbohydrate based fuel.</p><p>The maximum power density of the assembled β-glucan/O<sub>2</sub> biofuel cell reached 26.3 ± 4.6 μWcm<sup>−2</sup> at 0.36 V in phosphate buffer containing 0.5 % (w/v) β-glucan at 40 °C with excellent stability retaining 68.6 % of its initial performance after 5 days. The result confirms that β-glucan can be employed as fuel in an enzymatic biofuel cell.</p>}},
  author       = {{Rafighi, Parvin and Nordberg Karlsson, Eva and Zubaida Gulshan Ara, Kazi and Pankratova, Galina and Bollella, Paolo and Peterbauer, Clemens K. and Gorton, Lo}},
  issn         = {{1878-562X}},
  keywords     = {{Agaricales; Bioelectric Energy Sources; Electrodes; Enzymes, Immobilized; Glucose; Graphite; Osmium; Phosphates; Polymers; Rhodothermus; beta-Glucans; beta-Glucosidase}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Bioelectrochemistry}},
  title        = {{A novel membraneless β-glucan/O<sub>2</sub> enzymatic fuel cell based on β-glucosidase (<i>Rm</i>Bgl3B)/pyranose dehydrogenase (<i>Am</i>PDH) co-immobilized onto buckypaper electrode}},
  url          = {{http://dx.doi.org/10.1016/j.bioelechem.2022.108254}},
  doi          = {{10.1016/j.bioelechem.2022.108254}},
  volume       = {{148}},
  year         = {{2022}},
}