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A gene expression fingerprint of mouse stomach ECL cells

Andersson, N; Skrtic, S M; Håkanson, Rolf LU and Ohlsson, C (2005) In Biochemical and Biophysical Research Communications 332(2). p.404-410
Abstract
Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in all attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine... (More)
Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in all attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enterochromaffin-like cells, microarray, inbred C57BL/6, mice, stomach, gastric mucosa, ECL cells, parathyroid hormone-like hormone, parathyroid hormone-related peptide
in
Biochemical and Biophysical Research Communications
volume
332
issue
2
pages
404 - 410
publisher
Elsevier
external identifiers
  • pmid:15896709
  • wos:000229572300015
  • scopus:19444366893
ISSN
1090-2104
DOI
10.1016/j.bbrc.2005.05.004
language
English
LU publication?
yes
id
2abc4d7f-0d28-46ab-b370-c930c36934cb (old id 238816)
date added to LUP
2007-08-02 10:54:49
date last changed
2017-06-25 04:19:19
@article{2abc4d7f-0d28-46ab-b370-c930c36934cb,
  abstract     = {Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in all attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.},
  author       = {Andersson, N and Skrtic, S M and Håkanson, Rolf and Ohlsson, C},
  issn         = {1090-2104},
  keyword      = {enterochromaffin-like cells,microarray,inbred C57BL/6,mice,stomach,gastric mucosa,ECL cells,parathyroid hormone-like hormone,parathyroid hormone-related peptide},
  language     = {eng},
  number       = {2},
  pages        = {404--410},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {A gene expression fingerprint of mouse stomach ECL cells},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2005.05.004},
  volume       = {332},
  year         = {2005},
}