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Bacteria on catheters in patients undergoing peritoneal dialysis

Pihl, Maria; Davies, Julia R.; Johansson, Ann-Cathrine LU and Svensater, Gunnel (2013) In Peritoneal Dialysis International 33(1). p.51-59
Abstract
Background: Peritonitis is the leading cause of morbidity for peritoneal dialysis (PD) patients, and microbial biofilms have previously been identified on catheters from infected patients. However, few studies of catheters from patients without clinical signs of infection have been undertaken. The aim of the present study was to investigate the extent to which bacteria are present on catheters from PD patients with no symptoms of infection. Methods: Microbiologic culturing under aerobic and anaerobic conditions and confocal laser scanning microscopy were used to determine the distribution of bacteria on PD catheters from 15 patients without clinical signs of infection and on catheters from 2 infected patients. The 16S rRNA gene sequencing... (More)
Background: Peritonitis is the leading cause of morbidity for peritoneal dialysis (PD) patients, and microbial biofilms have previously been identified on catheters from infected patients. However, few studies of catheters from patients without clinical signs of infection have been undertaken. The aim of the present study was to investigate the extent to which bacteria are present on catheters from PD patients with no symptoms of infection. Methods: Microbiologic culturing under aerobic and anaerobic conditions and confocal laser scanning microscopy were used to determine the distribution of bacteria on PD catheters from 15 patients without clinical signs of infection and on catheters from 2 infected patients. The 16S rRNA gene sequencing technique was used to identify cultured bacteria.. Results: Bacteria were detected on 12 of the 15 catheters from patients without signs of infection and on the 2 catheters from infected patients. Single-species and mixed-microbial communities containing up to 5 species were present on both the inside and the outside along the whole length of the colonized catheters. The bacterial species most commonly found were the skin commensals Staphylococcus epidermidis and Propionibacterium acnes, followed by S. warneri and S. lugdunensis. The strains of these micro-organisms, particularly those of S. epidermidis, varied in phenotype with respect to their tolerance of the major classes of antibiotics. Conclusions: Bacteria were common on catheters from patients without symptoms of infection. Up to 4 different bacterial species were found in close association and may represent a risk factor for the future development of peritonitis in patients hosting such micro-organisms. Perit Dial Int 2013; 33(1):51-59 www.PDIConnect.com epub ahead of print: 01 Aug 2012 doi:10.3747/pdi.2011.00320 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biofilm, 16S rRNA gene sequencing, anaerobic culture, confocal, microscopy
in
Peritoneal Dialysis International
volume
33
issue
1
pages
51 - 59
publisher
Multimed Inc.
external identifiers
  • wos:000314186000008
  • scopus:84878969284
ISSN
1718-4304
DOI
10.3747/pdi.2011.00320
language
English
LU publication?
yes
id
23c496e4-5a31-42e5-93d5-d8e565b5feb4 (old id 3674799)
date added to LUP
2013-05-02 08:58:26
date last changed
2019-09-17 02:42:38
@article{23c496e4-5a31-42e5-93d5-d8e565b5feb4,
  abstract     = {Background: Peritonitis is the leading cause of morbidity for peritoneal dialysis (PD) patients, and microbial biofilms have previously been identified on catheters from infected patients. However, few studies of catheters from patients without clinical signs of infection have been undertaken. The aim of the present study was to investigate the extent to which bacteria are present on catheters from PD patients with no symptoms of infection. Methods: Microbiologic culturing under aerobic and anaerobic conditions and confocal laser scanning microscopy were used to determine the distribution of bacteria on PD catheters from 15 patients without clinical signs of infection and on catheters from 2 infected patients. The 16S rRNA gene sequencing technique was used to identify cultured bacteria.. Results: Bacteria were detected on 12 of the 15 catheters from patients without signs of infection and on the 2 catheters from infected patients. Single-species and mixed-microbial communities containing up to 5 species were present on both the inside and the outside along the whole length of the colonized catheters. The bacterial species most commonly found were the skin commensals Staphylococcus epidermidis and Propionibacterium acnes, followed by S. warneri and S. lugdunensis. The strains of these micro-organisms, particularly those of S. epidermidis, varied in phenotype with respect to their tolerance of the major classes of antibiotics. Conclusions: Bacteria were common on catheters from patients without symptoms of infection. Up to 4 different bacterial species were found in close association and may represent a risk factor for the future development of peritonitis in patients hosting such micro-organisms. Perit Dial Int 2013; 33(1):51-59 www.PDIConnect.com epub ahead of print: 01 Aug 2012 doi:10.3747/pdi.2011.00320},
  author       = {Pihl, Maria and Davies, Julia R. and Johansson, Ann-Cathrine and Svensater, Gunnel},
  issn         = {1718-4304},
  keyword      = {Biofilm,16S rRNA gene sequencing,anaerobic culture,confocal,microscopy},
  language     = {eng},
  number       = {1},
  pages        = {51--59},
  publisher    = {Multimed Inc.},
  series       = {Peritoneal Dialysis International},
  title        = {Bacteria on catheters in patients undergoing peritoneal dialysis},
  url          = {http://dx.doi.org/10.3747/pdi.2011.00320},
  volume       = {33},
  year         = {2013},
}