Phosphate permeases of Saccharomyces cerevisiae
(1998) In Biochimica et Biophysica Acta - Bioenergetics 1365(1-2). p.23-30- Abstract
The PHO84 and PHO89 genes of Saccharomyces cerevisiae encode two high- affinity phosphate cotransporters of the plasma membrane. Hydropathy analysis suggests a secondary structure arrangements of the proteins in 12 transmembrane domains. The derepressible Pho84 and Pho89 transporters appear to have characteristic similarities with the phosphate transporters of Neurospora crassa. The Pho84 protein catalyzes a proton-coupled phosphate transport at acidic pH, while the Pho89 protein catalyzes a sodium-dependent phosphate uptake at alkaline pH. The Pho84 transporter can be stably overproduced in the cytoplasmic membrane of Escherichia coli, purified and reconstituted in a functional state into proteoliposomes.
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- author
- B. L., Persson ; Berhe, A. ; Fristedt, U. ; Martinez, P. ; Pattison, J. ; Petersson, Jens LU and Weinander, R.
- publishing date
- 1998-06-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PHO regulation, Phosphate transporter, Plasma membrane transport, Saccharomyces cerevisiae
- in
- Biochimica et Biophysica Acta - Bioenergetics
- volume
- 1365
- issue
- 1-2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:9693717
- scopus:0032320415
- ISSN
- 0005-2728
- DOI
- 10.1016/S0005-2728(98)00037-1
- language
- English
- LU publication?
- no
- id
- 23fd7ec7-012d-43ec-adb6-e053943e2355
- date added to LUP
- 2019-05-21 14:17:00
- date last changed
- 2024-06-11 12:35:49
@article{23fd7ec7-012d-43ec-adb6-e053943e2355, abstract = {{<p>The PHO84 and PHO89 genes of Saccharomyces cerevisiae encode two high- affinity phosphate cotransporters of the plasma membrane. Hydropathy analysis suggests a secondary structure arrangements of the proteins in 12 transmembrane domains. The derepressible Pho84 and Pho89 transporters appear to have characteristic similarities with the phosphate transporters of Neurospora crassa. The Pho84 protein catalyzes a proton-coupled phosphate transport at acidic pH, while the Pho89 protein catalyzes a sodium-dependent phosphate uptake at alkaline pH. The Pho84 transporter can be stably overproduced in the cytoplasmic membrane of Escherichia coli, purified and reconstituted in a functional state into proteoliposomes.</p>}}, author = {{B. L., Persson and Berhe, A. and Fristedt, U. and Martinez, P. and Pattison, J. and Petersson, Jens and Weinander, R.}}, issn = {{0005-2728}}, keywords = {{PHO regulation; Phosphate transporter; Plasma membrane transport; Saccharomyces cerevisiae}}, language = {{eng}}, month = {{06}}, number = {{1-2}}, pages = {{23--30}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta - Bioenergetics}}, title = {{Phosphate permeases of Saccharomyces cerevisiae}}, url = {{http://dx.doi.org/10.1016/S0005-2728(98)00037-1}}, doi = {{10.1016/S0005-2728(98)00037-1}}, volume = {{1365}}, year = {{1998}}, }