Advanced

The humoral immune response to Helicobacter pylori infection in children with gastrointestinal symptoms

Janulaityte-Gunther, D; Kucinskiene, R; Kupcinskas, L; Pavilonis, A; Labanauskas, L; Cizauskas, A; Schmidt, U; Wadström, Torkel LU and Andersen, L P (2005) In Pathogens and Disease 44(2). p.205-212
Abstract
The prevalence of Helicobacter pylori is high in Eastern Europe. The purpose of this study was to estimate the prevalence of H. pylori in symptomatic Lithuanian children and to identify the infection by clinicopathological and serological analyses. One hundred sixteen symptomatic children (age 8-16) with gastritis and duodenal ulcer were included. Biopsies were histologically assessed according to the Sydney-System. Serum IgG antibodies against H. pylori were detected by an enzyme-linked immunosorbent assay (ELISA), using low molecular mass antigen. The western blot technique was used to detect serum antibodies against the cytotoxin-associated protein (CagA) using whole cell antigen. Histologically the prevalence of H. pylori infection was... (More)
The prevalence of Helicobacter pylori is high in Eastern Europe. The purpose of this study was to estimate the prevalence of H. pylori in symptomatic Lithuanian children and to identify the infection by clinicopathological and serological analyses. One hundred sixteen symptomatic children (age 8-16) with gastritis and duodenal ulcer were included. Biopsies were histologically assessed according to the Sydney-System. Serum IgG antibodies against H. pylori were detected by an enzyme-linked immunosorbent assay (ELISA), using low molecular mass antigen. The western blot technique was used to detect serum antibodies against the cytotoxin-associated protein (CagA) using whole cell antigen. Histologically the prevalence of H. pylori infection was 79% and not influenced by demographic factors. Mucosal inflammation and atrophy were associated with a H. pylori infection. Intestinal metaplasia was found in eight children, suggesting early H. pylori acquisition in life. Increased levels of IgG antibodies were detected in 57% of children. The prevalence of IgG antibodies was significantly higher in patients with duodenal ulcer compared to children with gastritis. Forty-four (67%) H. pylori-seropositive children had antibodies against CagA. Low molecular weight-ELISA and whole cell-western blot results were significantly associated with histopathology, the presence of duodenal ulcer and the CagA status. A high number of false seronegative cases were due to poor immunological responses in children and poor locally validated tests. The prevalence of H. pylori infection in Lithuanian children is higher compared to Western Europe. The infection is acquired in early life. Diagnosing H. pylori infection, serology is helpful, but endoscopy/histology remains as gold standard. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunoblot, ELISA, infection, Helicobacter pylori, IgG antibodies, lithuania, children
in
Pathogens and Disease
volume
44
issue
2
pages
205 - 212
publisher
Wiley-Blackwell
external identifiers
  • wos:000229259700015
  • pmid:15866217
  • scopus:24344457745
ISSN
2049-632X
DOI
10.1016/j.femsim.2005.02.005
language
English
LU publication?
yes
id
8258a815-ca78-4d09-b1db-ddbc2445804a (old id 240180)
date added to LUP
2007-09-28 10:07:45
date last changed
2017-01-01 04:59:24
@article{8258a815-ca78-4d09-b1db-ddbc2445804a,
  abstract     = {The prevalence of Helicobacter pylori is high in Eastern Europe. The purpose of this study was to estimate the prevalence of H. pylori in symptomatic Lithuanian children and to identify the infection by clinicopathological and serological analyses. One hundred sixteen symptomatic children (age 8-16) with gastritis and duodenal ulcer were included. Biopsies were histologically assessed according to the Sydney-System. Serum IgG antibodies against H. pylori were detected by an enzyme-linked immunosorbent assay (ELISA), using low molecular mass antigen. The western blot technique was used to detect serum antibodies against the cytotoxin-associated protein (CagA) using whole cell antigen. Histologically the prevalence of H. pylori infection was 79% and not influenced by demographic factors. Mucosal inflammation and atrophy were associated with a H. pylori infection. Intestinal metaplasia was found in eight children, suggesting early H. pylori acquisition in life. Increased levels of IgG antibodies were detected in 57% of children. The prevalence of IgG antibodies was significantly higher in patients with duodenal ulcer compared to children with gastritis. Forty-four (67%) H. pylori-seropositive children had antibodies against CagA. Low molecular weight-ELISA and whole cell-western blot results were significantly associated with histopathology, the presence of duodenal ulcer and the CagA status. A high number of false seronegative cases were due to poor immunological responses in children and poor locally validated tests. The prevalence of H. pylori infection in Lithuanian children is higher compared to Western Europe. The infection is acquired in early life. Diagnosing H. pylori infection, serology is helpful, but endoscopy/histology remains as gold standard.},
  author       = {Janulaityte-Gunther, D and Kucinskiene, R and Kupcinskas, L and Pavilonis, A and Labanauskas, L and Cizauskas, A and Schmidt, U and Wadström, Torkel and Andersen, L P},
  issn         = {2049-632X},
  keyword      = {immunoblot,ELISA,infection,Helicobacter pylori,IgG antibodies,lithuania,children},
  language     = {eng},
  number       = {2},
  pages        = {205--212},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {The humoral immune response to Helicobacter pylori infection in children with gastrointestinal symptoms},
  url          = {http://dx.doi.org/10.1016/j.femsim.2005.02.005},
  volume       = {44},
  year         = {2005},
}