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Presence and function of the calcitonin gene-related peptide receptor on rat pial arteries investigated in vitro and in vivo

Petersen, KA; Nilsson, E; Olesen, J and Edvinsson, Lars LU (2005) In Cephalalgia 25(6). p.424-432
Abstract
Calcitonin gene-related peptide (CGRP) and related peptides may be involved in migraine pathogenesis. To understand their vasomotor role in the cerebral circulation, we performed two studies, a pressurized arteriography study of the middle cerebral artery (MCA) and a genuine closed cranial window (gCCW) in vivo study. Using the pressurized arteriography model rat MCAs were mounted on micropipettes, pressurized to 85 mmHg and luminally perfused. The diameter responses to luminally and abluminally applied rat-alpha CGRP, rat-beta CGRP, amylin and adrenomedullin were compared with the resting diameter. Only abluminally applied CGRP induced dilation of the cerebral arteries; E-max for alpha CGRP and beta CGRP were 35 +/- 0.5% and 10.8 +/-... (More)
Calcitonin gene-related peptide (CGRP) and related peptides may be involved in migraine pathogenesis. To understand their vasomotor role in the cerebral circulation, we performed two studies, a pressurized arteriography study of the middle cerebral artery (MCA) and a genuine closed cranial window (gCCW) in vivo study. Using the pressurized arteriography model rat MCAs were mounted on micropipettes, pressurized to 85 mmHg and luminally perfused. The diameter responses to luminally and abluminally applied rat-alpha CGRP, rat-beta CGRP, amylin and adrenomedullin were compared with the resting diameter. Only abluminally applied CGRP induced dilation of the cerebral arteries; E-max for alpha CGRP and beta CGRP were 35 +/- 0.5% and 10.8 +/- 0.2%. These responses were blocked by CGRP(8-37). The gCCW model allowed videomicroscopic visualization of the pial vessels in anaesthetized rats. Changes in vessel diameter to intravenously administered alpha CGRP and beta CGRP were compared with pre-infusion baseline. Intravenous infusion of alpha CGRP and beta CGRP in the highest dose induced dilation of the cerebral cortical pial arteries/arterioles of 40.3 +/- 7.5% and 49.1 +/- 8.4%, respectively. However, this was probably secondary to a decrease in blood pressure of 44.8 +/- 3.3 mmHg and 49.2 +/- 3.3 mmHg. Our results suggest that CGRP receptors are probably functional on the smooth muscle cells and not on the endothelium of rat cerebral arteries. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
middle cerebral artery, muscle cells, endothelium and vascular smooth, CGRP receptor, cortical pial arteries
in
Cephalalgia
volume
25
issue
6
pages
424 - 432
publisher
Wiley-Blackwell
external identifiers
  • wos:000229192600004
  • pmid:15910566
  • scopus:20044394766
ISSN
0333-1024
DOI
10.1111/j.1468-2982.2005.00869.x
language
English
LU publication?
yes
id
69706d87-2a4a-4f2f-9bbe-cc44a6053e7a (old id 240299)
date added to LUP
2007-08-22 14:45:42
date last changed
2017-08-27 05:30:55
@article{69706d87-2a4a-4f2f-9bbe-cc44a6053e7a,
  abstract     = {Calcitonin gene-related peptide (CGRP) and related peptides may be involved in migraine pathogenesis. To understand their vasomotor role in the cerebral circulation, we performed two studies, a pressurized arteriography study of the middle cerebral artery (MCA) and a genuine closed cranial window (gCCW) in vivo study. Using the pressurized arteriography model rat MCAs were mounted on micropipettes, pressurized to 85 mmHg and luminally perfused. The diameter responses to luminally and abluminally applied rat-alpha CGRP, rat-beta CGRP, amylin and adrenomedullin were compared with the resting diameter. Only abluminally applied CGRP induced dilation of the cerebral arteries; E-max for alpha CGRP and beta CGRP were 35 +/- 0.5% and 10.8 +/- 0.2%. These responses were blocked by CGRP(8-37). The gCCW model allowed videomicroscopic visualization of the pial vessels in anaesthetized rats. Changes in vessel diameter to intravenously administered alpha CGRP and beta CGRP were compared with pre-infusion baseline. Intravenous infusion of alpha CGRP and beta CGRP in the highest dose induced dilation of the cerebral cortical pial arteries/arterioles of 40.3 +/- 7.5% and 49.1 +/- 8.4%, respectively. However, this was probably secondary to a decrease in blood pressure of 44.8 +/- 3.3 mmHg and 49.2 +/- 3.3 mmHg. Our results suggest that CGRP receptors are probably functional on the smooth muscle cells and not on the endothelium of rat cerebral arteries.},
  author       = {Petersen, KA and Nilsson, E and Olesen, J and Edvinsson, Lars},
  issn         = {0333-1024},
  keyword      = {middle cerebral artery,muscle cells,endothelium and vascular smooth,CGRP receptor,cortical pial arteries},
  language     = {eng},
  number       = {6},
  pages        = {424--432},
  publisher    = {Wiley-Blackwell},
  series       = {Cephalalgia},
  title        = {Presence and function of the calcitonin gene-related peptide receptor on rat pial arteries investigated in vitro and in vivo},
  url          = {http://dx.doi.org/10.1111/j.1468-2982.2005.00869.x},
  volume       = {25},
  year         = {2005},
}