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PARP14 Contributes to the Development of the Tumor-Associated Macrophage Phenotype

Sturniolo, Isotta ; Váróczy, Csongor ; Regdon, Zsolt ; Mázló, Anett ; Muzsai, Szabolcs ; Bácsi, Attila ; Intili, Giorgia ; Hegedűs, Csaba ; Boothby, Mark R. and Holechek, Jacob , et al. (2024) In International Journal of Molecular Sciences 25(7).
Abstract

Cancers reprogram macrophages (MΦs) to a tumor-growth-promoting TAM (tumor-associated MΦ) phenotype that is similar to the anti-inflammatory M2 phenotype. Poly(ADP-ribose) polymerase (PARP) enzymes regulate various aspects of MΦ biology, but their role in the development of TAM phenotype has not yet been investigated. Here, we show that the multispectral PARP inhibitor (PARPi) PJ34 and the PARP14 specific inhibitor MCD113 suppress the expression of M2 marker genes in IL-4-polarized primary murine MΦs, in THP-1 monocytic human MΦs, and in primary human monocyte-derived MΦs. MΦs isolated from PARP14 knockout mice showed a limited ability to differentiate to M2 cells. In a murine model of TAM polarization (4T1 breast carcinoma cell... (More)

Cancers reprogram macrophages (MΦs) to a tumor-growth-promoting TAM (tumor-associated MΦ) phenotype that is similar to the anti-inflammatory M2 phenotype. Poly(ADP-ribose) polymerase (PARP) enzymes regulate various aspects of MΦ biology, but their role in the development of TAM phenotype has not yet been investigated. Here, we show that the multispectral PARP inhibitor (PARPi) PJ34 and the PARP14 specific inhibitor MCD113 suppress the expression of M2 marker genes in IL-4-polarized primary murine MΦs, in THP-1 monocytic human MΦs, and in primary human monocyte-derived MΦs. MΦs isolated from PARP14 knockout mice showed a limited ability to differentiate to M2 cells. In a murine model of TAM polarization (4T1 breast carcinoma cell supernatant transfer to primary MΦs) and in a human TAM model (spheroids formed from JIMT-1 breast carcinoma cells and THP-1-MΦs), both PARPis and the PARP14 KO phenotype caused weaker TAM polarization. Increased JIMT-1 cell apoptosis in co-culture spheroids treated with PARPis suggested reduced functional TAM reprogramming. Protein profiling arrays identified lipocalin-2, macrophage migration inhibitory factor, and plasminogen activator inhibitor-1 as potential (ADP-ribosyl)ation-dependent mediators of TAM differentiation. Our data suggest that PARP14 inhibition might be a viable anticancer strategy with a potential to boost anticancer immune responses by reprogramming TAMs.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ADP-ribosylation, breast cancer, macrophage, PARP14
in
International Journal of Molecular Sciences
volume
25
issue
7
article number
3601
publisher
MDPI AG
external identifiers
  • scopus:85190369121
  • pmid:38612413
ISSN
1661-6596
DOI
10.3390/ijms25073601
language
English
LU publication?
yes
id
240c1d0d-8bce-4217-933d-26ad380035f7
date added to LUP
2024-05-03 10:25:30
date last changed
2024-06-28 15:47:44
@article{240c1d0d-8bce-4217-933d-26ad380035f7,
  abstract     = {{<p>Cancers reprogram macrophages (MΦs) to a tumor-growth-promoting TAM (tumor-associated MΦ) phenotype that is similar to the anti-inflammatory M2 phenotype. Poly(ADP-ribose) polymerase (PARP) enzymes regulate various aspects of MΦ biology, but their role in the development of TAM phenotype has not yet been investigated. Here, we show that the multispectral PARP inhibitor (PARPi) PJ34 and the PARP14 specific inhibitor MCD113 suppress the expression of M2 marker genes in IL-4-polarized primary murine MΦs, in THP-1 monocytic human MΦs, and in primary human monocyte-derived MΦs. MΦs isolated from PARP14 knockout mice showed a limited ability to differentiate to M2 cells. In a murine model of TAM polarization (4T1 breast carcinoma cell supernatant transfer to primary MΦs) and in a human TAM model (spheroids formed from JIMT-1 breast carcinoma cells and THP-1-MΦs), both PARPis and the PARP14 KO phenotype caused weaker TAM polarization. Increased JIMT-1 cell apoptosis in co-culture spheroids treated with PARPis suggested reduced functional TAM reprogramming. Protein profiling arrays identified lipocalin-2, macrophage migration inhibitory factor, and plasminogen activator inhibitor-1 as potential (ADP-ribosyl)ation-dependent mediators of TAM differentiation. Our data suggest that PARP14 inhibition might be a viable anticancer strategy with a potential to boost anticancer immune responses by reprogramming TAMs.</p>}},
  author       = {{Sturniolo, Isotta and Váróczy, Csongor and Regdon, Zsolt and Mázló, Anett and Muzsai, Szabolcs and Bácsi, Attila and Intili, Giorgia and Hegedűs, Csaba and Boothby, Mark R. and Holechek, Jacob and Ferraris, Dana and Schüler, Herwig and Virág, László}},
  issn         = {{1661-6596}},
  keywords     = {{ADP-ribosylation; breast cancer; macrophage; PARP14}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{PARP14 Contributes to the Development of the Tumor-Associated Macrophage Phenotype}},
  url          = {{http://dx.doi.org/10.3390/ijms25073601}},
  doi          = {{10.3390/ijms25073601}},
  volume       = {{25}},
  year         = {{2024}},
}