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Long-term effect of apatite on ectomycorrhizal growth and community structure.

Berner, Christoffer LU ; Johansson, Tomas LU and Wallander, Håkan LU (2012) In Mycorrhiza 22(8). p.615-621
Abstract
Ectomycorrhizal (ECM) fungi are efficient at taking up phosphorus (P) from mineral sources, such as apatite, which are not easily available to the host trees. Since ECM fungal species differ in P uptake rates, it can be expected that the composition of the ECM fungal community will change upon exposure to apatite, provided that the P transfer is rewarded by more carbon being transferred to the fungal symbiont. Control and apatite-amended mesh bags were buried in pairs in the humus layer of a P-poor Norway spruce forest. The ECM fungal community that colonized these bags was analyzed by DNA extraction, PCR amplification of the internal transcribed spacer (ITS) region, cloning, and random sequencing. Fungal biomass was estimated by... (More)
Ectomycorrhizal (ECM) fungi are efficient at taking up phosphorus (P) from mineral sources, such as apatite, which are not easily available to the host trees. Since ECM fungal species differ in P uptake rates, it can be expected that the composition of the ECM fungal community will change upon exposure to apatite, provided that the P transfer is rewarded by more carbon being transferred to the fungal symbiont. Control and apatite-amended mesh bags were buried in pairs in the humus layer of a P-poor Norway spruce forest. The ECM fungal community that colonized these bags was analyzed by DNA extraction, PCR amplification of the internal transcribed spacer (ITS) region, cloning, and random sequencing. Fungal biomass was estimated by ergosterol analysis. No change in the ECM fungal community structure was seen after 5 years of apatite exposure, although the fungal biomass increased threefold upon apatite amendment. Our results indicate that host trees enhance carbon allocation to ECM fungi colonizing P sources in P-poor forests but the lack of change in the composition of the ECM fungal community suggests that P transfer rates were similar among the species. Alternatively, higher P transfer among certain species was not rewarded with higher carbon transfer from the host. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ectomycorrhiza, Community structure, Apatite, Norway spruce, External mycelium, Weathering
in
Mycorrhiza
volume
22
issue
8
pages
615 - 621
publisher
Springer
external identifiers
  • wos:000310324600004
  • pmid:22451218
  • scopus:84867981640
ISSN
1432-1890
DOI
10.1007/s00572-012-0438-y
project
Ectomycorrhizal fungi and apatite weathering
Ectomycorrhizal fungi and nutrient mobilisation
BECC
language
English
LU publication?
yes
id
b3c74bd3-032e-49ac-a478-7a2b57a160e7 (old id 2431418)
date added to LUP
2012-05-08 10:50:59
date last changed
2017-10-22 03:31:36
@article{b3c74bd3-032e-49ac-a478-7a2b57a160e7,
  abstract     = {Ectomycorrhizal (ECM) fungi are efficient at taking up phosphorus (P) from mineral sources, such as apatite, which are not easily available to the host trees. Since ECM fungal species differ in P uptake rates, it can be expected that the composition of the ECM fungal community will change upon exposure to apatite, provided that the P transfer is rewarded by more carbon being transferred to the fungal symbiont. Control and apatite-amended mesh bags were buried in pairs in the humus layer of a P-poor Norway spruce forest. The ECM fungal community that colonized these bags was analyzed by DNA extraction, PCR amplification of the internal transcribed spacer (ITS) region, cloning, and random sequencing. Fungal biomass was estimated by ergosterol analysis. No change in the ECM fungal community structure was seen after 5 years of apatite exposure, although the fungal biomass increased threefold upon apatite amendment. Our results indicate that host trees enhance carbon allocation to ECM fungi colonizing P sources in P-poor forests but the lack of change in the composition of the ECM fungal community suggests that P transfer rates were similar among the species. Alternatively, higher P transfer among certain species was not rewarded with higher carbon transfer from the host.},
  author       = {Berner, Christoffer and Johansson, Tomas and Wallander, Håkan},
  issn         = {1432-1890},
  keyword      = {Ectomycorrhiza,Community structure,Apatite,Norway spruce,External mycelium,Weathering},
  language     = {eng},
  number       = {8},
  pages        = {615--621},
  publisher    = {Springer},
  series       = {Mycorrhiza},
  title        = {Long-term effect of apatite on ectomycorrhizal growth and community structure.},
  url          = {http://dx.doi.org/10.1007/s00572-012-0438-y},
  volume       = {22},
  year         = {2012},
}