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FOSL1 as a candidate target gene for 11q12 rearrangements in desmoplastic fibroblastoma.

Macchia, Gemma; Trombetta, Domenico; Möller, Emely LU ; Mertens, Fredrik LU ; Storlazzi, Clelia T; Debiec-Rychter, Maria; Sciot, Raf and Hansén Nord, Karolin LU (2012) In Laboratory Investigation 92(5). p.735-743
Abstract
Desmoplastic fibroblastoma (DF) is a benign fibroblastic/myofibroblastic tumor. Cytogenetic analyses have revealed consistent rearrangement of chromosome band 11q12, strongly suggesting that this region harbors a gene of pathogenetic importance. To identify the target gene of the 11q12 rearrangements, we analyzed six cases diagnosed as DF using chromosome banding, fluorescence in situ hybridization (FISH), single-nucleotide polymorphism array and gene expression approaches. Different structural rearrangements involving 11q12 were found in five of the six cases. Metaphase FISH analyses in two of them mapped the 11q12 breakpoints to an ∼20-kb region, harboring FOSL1. Global gene expression profiling followed by quantitative real-time PCR... (More)
Desmoplastic fibroblastoma (DF) is a benign fibroblastic/myofibroblastic tumor. Cytogenetic analyses have revealed consistent rearrangement of chromosome band 11q12, strongly suggesting that this region harbors a gene of pathogenetic importance. To identify the target gene of the 11q12 rearrangements, we analyzed six cases diagnosed as DF using chromosome banding, fluorescence in situ hybridization (FISH), single-nucleotide polymorphism array and gene expression approaches. Different structural rearrangements involving 11q12 were found in five of the six cases. Metaphase FISH analyses in two of them mapped the 11q12 breakpoints to an ∼20-kb region, harboring FOSL1. Global gene expression profiling followed by quantitative real-time PCR showed that FOSL1 was expressed at higher levels in DF with 11q12 rearrangements than in desmoid-type fibromatoses. Furthermore, FOSL1 was not upregulated in the single case of DF that did not show cytogenetic involvement of 11q12; instead this tumor was found to display a hemizygous loss on 5q, including the APC (adenomatous polyposis coli) locus, raising the possibility that it actually was a misdiagnosed Gardner fibroma. 5'RACE-PCR in two 11q12-positive DF did not identify any fusion transcripts. Thus, in agreement with the finding at chromosome banding analysis that varying translocation partners are involved in the 11q12 rearrangement, the molecular data suggest that the functional outcome of the 11q12 rearrangements is deregulated expression of FOSL1.Laboratory Investigation advance online publication, 12 March 2012; doi:10.1038/labinvest.2012.46. (Less)
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Contribution to journal
publication status
published
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in
Laboratory Investigation
volume
92
issue
5
pages
735 - 743
publisher
Nature Publishing Group
external identifiers
  • wos:000303366200008
  • pmid:22411068
  • scopus:84860290499
ISSN
1530-0307
DOI
10.1038/labinvest.2012.46
language
English
LU publication?
yes
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eafd96c5-8dfd-4ead-bd82-52e691d44648 (old id 2431899)
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http://www.ncbi.nlm.nih.gov/pubmed/22411068?dopt=Abstract
date added to LUP
2012-04-03 10:39:24
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2017-09-24 04:39:29
@article{eafd96c5-8dfd-4ead-bd82-52e691d44648,
  abstract     = {Desmoplastic fibroblastoma (DF) is a benign fibroblastic/myofibroblastic tumor. Cytogenetic analyses have revealed consistent rearrangement of chromosome band 11q12, strongly suggesting that this region harbors a gene of pathogenetic importance. To identify the target gene of the 11q12 rearrangements, we analyzed six cases diagnosed as DF using chromosome banding, fluorescence in situ hybridization (FISH), single-nucleotide polymorphism array and gene expression approaches. Different structural rearrangements involving 11q12 were found in five of the six cases. Metaphase FISH analyses in two of them mapped the 11q12 breakpoints to an ∼20-kb region, harboring FOSL1. Global gene expression profiling followed by quantitative real-time PCR showed that FOSL1 was expressed at higher levels in DF with 11q12 rearrangements than in desmoid-type fibromatoses. Furthermore, FOSL1 was not upregulated in the single case of DF that did not show cytogenetic involvement of 11q12; instead this tumor was found to display a hemizygous loss on 5q, including the APC (adenomatous polyposis coli) locus, raising the possibility that it actually was a misdiagnosed Gardner fibroma. 5'RACE-PCR in two 11q12-positive DF did not identify any fusion transcripts. Thus, in agreement with the finding at chromosome banding analysis that varying translocation partners are involved in the 11q12 rearrangement, the molecular data suggest that the functional outcome of the 11q12 rearrangements is deregulated expression of FOSL1.Laboratory Investigation advance online publication, 12 March 2012; doi:10.1038/labinvest.2012.46.},
  author       = {Macchia, Gemma and Trombetta, Domenico and Möller, Emely and Mertens, Fredrik and Storlazzi, Clelia T and Debiec-Rychter, Maria and Sciot, Raf and Hansén Nord, Karolin},
  issn         = {1530-0307},
  language     = {eng},
  number       = {5},
  pages        = {735--743},
  publisher    = {Nature Publishing Group},
  series       = {Laboratory Investigation},
  title        = {FOSL1 as a candidate target gene for 11q12 rearrangements in desmoplastic fibroblastoma.},
  url          = {http://dx.doi.org/10.1038/labinvest.2012.46},
  volume       = {92},
  year         = {2012},
}