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Pseudomonas aeruginosa population structure revisited under environmental focus: impact of water quality and phage pressure.

Selezska, Katherina; Kazmierczak, Marlon; Müsken, Mathias; Garbe, Julia LU ; Schobert, Max; Häussler, Susanne; Wiehlmann, Lutz; Rohde, Christine and Sikorski, Johannes (2012) In Environmental Microbiology 14(8). p.1952-1967
Abstract
Pseudomonas aeruginosa attracts research attention as a common opportunistic nosocomial pathogen causing severe health problems in humans. Nevertheless, its primary habitat is the natural environment. Here, we relate the genetic diversity of 381 environmental isolates from rivers in northern Germany to ecological factors such as river system, season of sampling and different levels of water quality. From representatives of 99 environmental clones, also in comparison with 91 clinical isolates, we determined motility phenotypes, virulence factors, biofilm formation, serotype and the resistance to seven environmental P. aeruginosa phages. The integration of genetic, ecological and phenotypic data showed (i) the presence of several extended... (More)
Pseudomonas aeruginosa attracts research attention as a common opportunistic nosocomial pathogen causing severe health problems in humans. Nevertheless, its primary habitat is the natural environment. Here, we relate the genetic diversity of 381 environmental isolates from rivers in northern Germany to ecological factors such as river system, season of sampling and different levels of water quality. From representatives of 99 environmental clones, also in comparison with 91 clinical isolates, we determined motility phenotypes, virulence factors, biofilm formation, serotype and the resistance to seven environmental P. aeruginosa phages. The integration of genetic, ecological and phenotypic data showed (i) the presence of several extended clonal complexes (ecc) which are non-uniformly distributed across different water qualities, and (ii) a correlation of the hosts' serotype composition with susceptibility towards distinct groups of environmental phages. For at least one ecc (eccB), we assumed the ecophysiological differences on environmental water adaptation and phage resistance to be so distinct as to reinforce an environmentally driven cladogenic split from the remainder of P. aeruginosa. In summary, we conclude that the majority of the microevolutionary population dynamics of P. aeruginosa were shaped by the natural environment and not by the clinical habitat. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Microbiology
volume
14
issue
8
pages
1952 - 1967
publisher
Wiley-Blackwell
external identifiers
  • wos:000306899900012
  • pmid:22390474
  • scopus:84864387005
ISSN
1462-2920
DOI
10.1111/j.1462-2920.2012.02719.x
language
English
LU publication?
yes
id
2b96d17f-eee1-41cb-93e6-69c6c41edd8d (old id 2432256)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22390474?dopt=Abstract
date added to LUP
2012-04-03 08:45:52
date last changed
2017-11-12 04:07:36
@article{2b96d17f-eee1-41cb-93e6-69c6c41edd8d,
  abstract     = {Pseudomonas aeruginosa attracts research attention as a common opportunistic nosocomial pathogen causing severe health problems in humans. Nevertheless, its primary habitat is the natural environment. Here, we relate the genetic diversity of 381 environmental isolates from rivers in northern Germany to ecological factors such as river system, season of sampling and different levels of water quality. From representatives of 99 environmental clones, also in comparison with 91 clinical isolates, we determined motility phenotypes, virulence factors, biofilm formation, serotype and the resistance to seven environmental P. aeruginosa phages. The integration of genetic, ecological and phenotypic data showed (i) the presence of several extended clonal complexes (ecc) which are non-uniformly distributed across different water qualities, and (ii) a correlation of the hosts' serotype composition with susceptibility towards distinct groups of environmental phages. For at least one ecc (eccB), we assumed the ecophysiological differences on environmental water adaptation and phage resistance to be so distinct as to reinforce an environmentally driven cladogenic split from the remainder of P. aeruginosa. In summary, we conclude that the majority of the microevolutionary population dynamics of P. aeruginosa were shaped by the natural environment and not by the clinical habitat.},
  author       = {Selezska, Katherina and Kazmierczak, Marlon and Müsken, Mathias and Garbe, Julia and Schobert, Max and Häussler, Susanne and Wiehlmann, Lutz and Rohde, Christine and Sikorski, Johannes},
  issn         = {1462-2920},
  language     = {eng},
  number       = {8},
  pages        = {1952--1967},
  publisher    = {Wiley-Blackwell},
  series       = {Environmental Microbiology},
  title        = {Pseudomonas aeruginosa population structure revisited under environmental focus: impact of water quality and phage pressure.},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2012.02719.x},
  volume       = {14},
  year         = {2012},
}