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Growth and hydrogen production characteristics of Caldicellulosiruptor saccharolyticus on chemically defined minimal media

Willquist, Karin LU and van Niel, Ed LU (2012) In International Journal of Hydrogen Energy 37(6). p.4925-4929
Abstract
Caldicellulosiruptor saccharolyticus is an extreme thermophilic bacterium recognized for its saccharolytic ability and superior ability to produce high yields of hydrogen. However,most studies have been made using yeast extract (YE) as a rich but expensive nutrient

source. For the first time, we show that C. saccharolyticus is able to grow on defined minimal media, including essential vitamins, provided that CO2 was allowed to accumulate sufficiently

in the culture broth to activate growth. Growth and hydrogen production performance on minimal media was analyzed in both batch and continuous mode. Absence of YE resulted in similar or higher hydrogen yields and specific hydrogen productivities but lower

volumetric... (More)
Caldicellulosiruptor saccharolyticus is an extreme thermophilic bacterium recognized for its saccharolytic ability and superior ability to produce high yields of hydrogen. However,most studies have been made using yeast extract (YE) as a rich but expensive nutrient

source. For the first time, we show that C. saccharolyticus is able to grow on defined minimal media, including essential vitamins, provided that CO2 was allowed to accumulate sufficiently

in the culture broth to activate growth. Growth and hydrogen production performance on minimal media was analyzed in both batch and continuous mode. Absence of YE resulted in similar or higher hydrogen yields and specific hydrogen productivities but lower

volumetric hydrogen productivities than with YE. The results also indicate that YE is used as a carbon- and energy source thus affecting metabolic flux calculations. This study clarified that YE is not essential making C. saccharolyticus more attractive for fundamental studies on its metabolism and future industrial exploitation. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Caldicellulosiruptor saccharolyticus Minimal media Hydrogen production Growth activation CO2 sparging
in
International Journal of Hydrogen Energy
volume
37
issue
6
pages
4925 - 4929
publisher
Elsevier
external identifiers
  • wos:000301990100019
  • scopus:84857650586
ISSN
1879-3487
DOI
10.1016/j.ijhydene.2011.12.055
language
English
LU publication?
yes
id
902dea30-6521-4e59-9e9a-0acda78da63b (old id 2439075)
date added to LUP
2012-04-16 12:54:34
date last changed
2017-01-01 06:00:12
@article{902dea30-6521-4e59-9e9a-0acda78da63b,
  abstract     = {Caldicellulosiruptor saccharolyticus is an extreme thermophilic bacterium recognized for its saccharolytic ability and superior ability to produce high yields of hydrogen. However,most studies have been made using yeast extract (YE) as a rich but expensive nutrient<br/><br>
source. For the first time, we show that C. saccharolyticus is able to grow on defined minimal media, including essential vitamins, provided that CO2 was allowed to accumulate sufficiently<br/><br>
in the culture broth to activate growth. Growth and hydrogen production performance on minimal media was analyzed in both batch and continuous mode. Absence of YE resulted in similar or higher hydrogen yields and specific hydrogen productivities but lower<br/><br>
volumetric hydrogen productivities than with YE. The results also indicate that YE is used as a carbon- and energy source thus affecting metabolic flux calculations. This study clarified that YE is not essential making C. saccharolyticus more attractive for fundamental studies on its metabolism and future industrial exploitation.},
  author       = {Willquist, Karin and van Niel, Ed},
  issn         = {1879-3487},
  keyword      = {Caldicellulosiruptor saccharolyticus Minimal media Hydrogen production Growth activation CO2 sparging},
  language     = {eng},
  number       = {6},
  pages        = {4925--4929},
  publisher    = {Elsevier},
  series       = {International Journal of Hydrogen Energy},
  title        = {Growth and hydrogen production characteristics of Caldicellulosiruptor saccharolyticus on chemically defined minimal media},
  url          = {http://dx.doi.org/10.1016/j.ijhydene.2011.12.055},
  volume       = {37},
  year         = {2012},
}