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Assignment of vibrational spectral bands of kidney tissue by means of low temperature sers spectroscopy

Velicka, M.; Radzvilaite, M.; Ceponkus, Justinas; Urboniene, V.; Pucetaite, M. LU ; Jankevicius, F.; Steiner, Gerald and Sablinskas, V. (2017) Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV In Proceedings of SPIE 10068.
Abstract

Surface enhanced Raman scattering (SERS) spectroscopy is a useful method for detection of trace amounts of molecules. It has already been successfully implemented for detection of explosives, food additives, biomarkers in blood or urine, etc. In the last decade, SERS spectroscopy was introduced into the field of health sciences and has been especially focused on early disease detection. In the recent years, application of SERS spectroscopy for detection of various types of human cancerous tissues emerged. Furthermore, SERS spectroscopy of extracellular fluid shows great potential for the differentiation of normal and cancerous tissues; however, due to high variety of molecules present in such biological samples, the experimental... (More)

Surface enhanced Raman scattering (SERS) spectroscopy is a useful method for detection of trace amounts of molecules. It has already been successfully implemented for detection of explosives, food additives, biomarkers in blood or urine, etc. In the last decade, SERS spectroscopy was introduced into the field of health sciences and has been especially focused on early disease detection. In the recent years, application of SERS spectroscopy for detection of various types of human cancerous tissues emerged. Furthermore, SERS spectroscopy of extracellular fluid shows great potential for the differentiation of normal and cancerous tissues; however, due to high variety of molecules present in such biological samples, the experimental spectrum is a combination of many different overlapping vibrational spectral bands. Thus, precise assignment of these bands to the corresponding molecular vibrations is a difficult task. In most cases, researchers try to avoid this task satisfying just with tentative assignment. In this study, low temperature SERS measurements of extracellular fluid of cancerous and healthy kidney tissue samples were carried out in order to get a deeper understanding of the nature of vibrational spectral bands present in the experimental spectrum. The SERS spectra were measured in temperature range from 300 K down to 100 K. SERS method was implemented using silver nanoparticle colloidal solution. The results of the low temperature SERS experiment were analysed and compared with the results of theoretical calculations. The analysis showed that the SERS spectrum of extracellular fluid of kidney tissue is highly influenced by the vibrational bands of adenine and Lcystine molecules.

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Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Extracellular fluid, Kidney cancer, Low temperature, SERS
host publication
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
series title
Proceedings of SPIE
editor
Farkas, Daniel L.; Nicolau, Dan V.; Leif, Robert C.; ; and
volume
10068
publisher
SPIE
conference name
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
conference location
San Francisco, United States
conference dates
2017-01-30 - 2017-02-01
external identifiers
  • scopus:85020435396
ISSN
1996-756X
0277-786X
ISBN
9781510605770
DOI
10.1117/12.2252907
language
English
LU publication?
no
id
2442c786-7321-4200-b38d-675b0b026e67
date added to LUP
2019-03-18 11:54:08
date last changed
2019-03-21 15:14:13
@inproceedings{2442c786-7321-4200-b38d-675b0b026e67,
  abstract     = {<p>Surface enhanced Raman scattering (SERS) spectroscopy is a useful method for detection of trace amounts of molecules. It has already been successfully implemented for detection of explosives, food additives, biomarkers in blood or urine, etc. In the last decade, SERS spectroscopy was introduced into the field of health sciences and has been especially focused on early disease detection. In the recent years, application of SERS spectroscopy for detection of various types of human cancerous tissues emerged. Furthermore, SERS spectroscopy of extracellular fluid shows great potential for the differentiation of normal and cancerous tissues; however, due to high variety of molecules present in such biological samples, the experimental spectrum is a combination of many different overlapping vibrational spectral bands. Thus, precise assignment of these bands to the corresponding molecular vibrations is a difficult task. In most cases, researchers try to avoid this task satisfying just with tentative assignment. In this study, low temperature SERS measurements of extracellular fluid of cancerous and healthy kidney tissue samples were carried out in order to get a deeper understanding of the nature of vibrational spectral bands present in the experimental spectrum. The SERS spectra were measured in temperature range from 300 K down to 100 K. SERS method was implemented using silver nanoparticle colloidal solution. The results of the low temperature SERS experiment were analysed and compared with the results of theoretical calculations. The analysis showed that the SERS spectrum of extracellular fluid of kidney tissue is highly influenced by the vibrational bands of adenine and Lcystine molecules.</p>},
  author       = {Velicka, M. and Radzvilaite, M. and Ceponkus, Justinas and Urboniene, V. and Pucetaite, M. and Jankevicius, F. and Steiner, Gerald and Sablinskas, V.},
  booktitle    = {Proceedings of SPIE },
  editor       = {Farkas, Daniel L. and Nicolau, Dan V. and Leif, Robert C.},
  isbn         = {9781510605770},
  issn         = {1996-756X},
  keyword      = {Extracellular fluid,Kidney cancer,Low temperature,SERS},
  language     = {eng},
  location     = {San Francisco, United States},
  month        = {01},
  publisher    = {SPIE},
  title        = {Assignment of vibrational spectral bands of kidney tissue by means of low temperature sers spectroscopy},
  url          = {http://dx.doi.org/10.1117/12.2252907},
  volume       = {10068},
  year         = {2017},
}