Advanced

Light-activated desorption of photoactive polyelectrolytes from supported lipid bilayers

Benkoski, JJ; Jesorka, A; Kasemo, B and Höök, Fredrik LU (2005) In Macromolecules 38(9). p.3852-3860
Abstract
Phospholipid vesicles and supported bilayers have emerged as a promising platform for the development of biorecognition devices. To expand the capabilities of such biochips, it becomes desirable to direct and control the assembly of lipid structures into more sophisticated architectures. As one step toward this goal, we demonstrate the photoregulated desorption of a new class of polymer from lipid bilayers. The neutral, hydrophobic polymer resides within the bilayer under mild pH and ambient conditions. However, it contains side groups that can undergo excited state proton transfer (ESPT). The polymer therefore behaves as a polyelectrolyte when exposed to IN light. With the ensuing increase in hydrophilicity, the molecule is spontaneously... (More)
Phospholipid vesicles and supported bilayers have emerged as a promising platform for the development of biorecognition devices. To expand the capabilities of such biochips, it becomes desirable to direct and control the assembly of lipid structures into more sophisticated architectures. As one step toward this goal, we demonstrate the photoregulated desorption of a new class of polymer from lipid bilayers. The neutral, hydrophobic polymer resides within the bilayer under mild pH and ambient conditions. However, it contains side groups that can undergo excited state proton transfer (ESPT). The polymer therefore behaves as a polyelectrolyte when exposed to IN light. With the ensuing increase in hydrophilicity, the molecule is spontaneously ejected from the bilayer. Quartz crystal microbalance measurements with dissipation monitoring (QCM-D) have recorded this process and have shown that a rapid buffer exchange during light exposure results in efficient removal of the polymer from the system. Three polymers were tested in all: a polyanion, a polyeation, and a polyzwitterion. A one-step approach to the synthesis of the monomer, performed under relatively mild reaction conditions, made it possible to synthesize each polymer in one step. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Macromolecules
volume
38
issue
9
pages
3852 - 3860
publisher
The American Chemical Society
external identifiers
  • wos:000228738600044
  • scopus:18844447496
ISSN
0024-9297
DOI
10.1021/ma048046q
language
English
LU publication?
yes
id
4e53b2f6-7adc-40d3-8cbd-968d7d5a653a (old id 244309)
date added to LUP
2007-08-16 11:50:11
date last changed
2017-01-01 04:19:52
@article{4e53b2f6-7adc-40d3-8cbd-968d7d5a653a,
  abstract     = {Phospholipid vesicles and supported bilayers have emerged as a promising platform for the development of biorecognition devices. To expand the capabilities of such biochips, it becomes desirable to direct and control the assembly of lipid structures into more sophisticated architectures. As one step toward this goal, we demonstrate the photoregulated desorption of a new class of polymer from lipid bilayers. The neutral, hydrophobic polymer resides within the bilayer under mild pH and ambient conditions. However, it contains side groups that can undergo excited state proton transfer (ESPT). The polymer therefore behaves as a polyelectrolyte when exposed to IN light. With the ensuing increase in hydrophilicity, the molecule is spontaneously ejected from the bilayer. Quartz crystal microbalance measurements with dissipation monitoring (QCM-D) have recorded this process and have shown that a rapid buffer exchange during light exposure results in efficient removal of the polymer from the system. Three polymers were tested in all: a polyanion, a polyeation, and a polyzwitterion. A one-step approach to the synthesis of the monomer, performed under relatively mild reaction conditions, made it possible to synthesize each polymer in one step.},
  author       = {Benkoski, JJ and Jesorka, A and Kasemo, B and Höök, Fredrik},
  issn         = {0024-9297},
  language     = {eng},
  number       = {9},
  pages        = {3852--3860},
  publisher    = {The American Chemical Society},
  series       = {Macromolecules},
  title        = {Light-activated desorption of photoactive polyelectrolytes from supported lipid bilayers},
  url          = {http://dx.doi.org/10.1021/ma048046q},
  volume       = {38},
  year         = {2005},
}