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Fabrication and optimization of stable, optically transparent, and reusable ph-responsive silk membranes

Toytziaridis, Andreas and Dicko, Cedric LU (2016) In International Journal of Molecular Sciences 17(11).
Abstract

The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of... (More)

The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of Thionine and 15 mg/mL silk, were further tested for operational stability over several cycles of pH altering. Stability, performance, and reusability were achieved over the tested cycles. The approach could be extended to other reporting molecules or enzymes able to bind to Laponite.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chromogenic, Laponite, Membrane, Neutral red, PH-responsive, Silk patch, Thionine
in
International Journal of Molecular Sciences
volume
17
issue
11
publisher
MOLECULAR DIVERSITY PRESERVATION INT
external identifiers
  • scopus:84995665652
  • wos:000388809600129
ISSN
1661-6596
DOI
10.3390/ijms17111897
language
English
LU publication?
yes
id
244a8841-5914-4614-9b5a-8bf909f8dd9b
date added to LUP
2016-12-05 08:10:19
date last changed
2017-09-18 11:29:48
@article{244a8841-5914-4614-9b5a-8bf909f8dd9b,
  abstract     = {<p>The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of Thionine and 15 mg/mL silk, were further tested for operational stability over several cycles of pH altering. Stability, performance, and reusability were achieved over the tested cycles. The approach could be extended to other reporting molecules or enzymes able to bind to Laponite.</p>},
  articleno    = {1897},
  author       = {Toytziaridis, Andreas and Dicko, Cedric},
  issn         = {1661-6596},
  keyword      = {Chromogenic,Laponite,Membrane,Neutral red,PH-responsive,Silk patch,Thionine},
  language     = {eng},
  month        = {11},
  number       = {11},
  publisher    = {MOLECULAR DIVERSITY PRESERVATION INT},
  series       = {International Journal of Molecular Sciences},
  title        = {Fabrication and optimization of stable, optically transparent, and reusable ph-responsive silk membranes},
  url          = {http://dx.doi.org/10.3390/ijms17111897},
  volume       = {17},
  year         = {2016},
}