Fabrication and optimization of stable, optically transparent, and reusable ph-responsive silk membranes
Toytziaridis, Andreas and Dicko, Cedric LU
(2016)
In International Journal of Molecular Sciences
17(11).
- Abstract
The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of... (More)
The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of Thionine and 15 mg/mL silk, were further tested for operational stability over several cycles of pH altering. Stability, performance, and reusability were achieved over the tested cycles. The approach could be extended to other reporting molecules or enzymes able to bind to Laponite.
(Less)
- author
- Toytziaridis, Andreas
and Dicko, Cedric
LU
- organization
- publishing date
- 2016-11-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chromogenic, Laponite, Membrane, Neutral red, PH-responsive, Silk patch, Thionine
- in
- International Journal of Molecular Sciences
- volume
- 17
- issue
- 11
- article number
- 1897
- publisher
- MDPI AG
- external identifiers
-
- scopus:84995665652
- pmid:27854303
- wos:000388809600129
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms17111897
- language
- English
- LU publication?
- yes
- id
- 244a8841-5914-4614-9b5a-8bf909f8dd9b
- date added to LUP
- 2016-12-05 08:10:19
- date last changed
- 2024-07-12 21:43:11
@article{244a8841-5914-4614-9b5a-8bf909f8dd9b,
abstract = {{<p>The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 µM neutral red or 250 µM of Thionine and 15 mg/mL silk, were further tested for operational stability over several cycles of pH altering. Stability, performance, and reusability were achieved over the tested cycles. The approach could be extended to other reporting molecules or enzymes able to bind to Laponite.</p>}},
author = {{Toytziaridis, Andreas and Dicko, Cedric}},
issn = {{1661-6596}},
keywords = {{Chromogenic; Laponite; Membrane; Neutral red; PH-responsive; Silk patch; Thionine}},
language = {{eng}},
month = {{11}},
number = {{11}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Fabrication and optimization of stable, optically transparent, and reusable ph-responsive silk membranes}},
url = {{http://dx.doi.org/10.3390/ijms17111897}},
doi = {{10.3390/ijms17111897}},
volume = {{17}},
year = {{2016}},
}