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Koyunlarda Haemonchus contortus’un moleküler prevalansı, filogenetik karakterizasyonu ve benzimidazol dirençliliği

Önder, Zuhal; Yildirim, Alparslan; İnci, Abdullah; Düzlü, Önder and Çiloğlu, Arif LU (2016) In Kafkas Universitesi Veteriner Fakultesi Dergisi 22(1). p.93-99
Abstract

This study was conducted to determine the molecular prevalence and characterization of Haemonchus contortus from sheep along with the benzimidazole (BZ) resistance in H. contortus populations. Fecal samples were collected from a total of 300 sheep in research area and analyzed by fecal flotation. qPCR assays were utilized on trichostrongylid egg positive samples in order to identify H. contortus. Phylogenetic analyses were performed on ribosomal ITS-2 and mt-COI gene regions. BZ sensitive and resistant allele frequencies were determined by qPCR along with sequence analyses of the ß-tubulin isotype 1 gene for single nucleotide polimorphizms (SNPs). H. contortus was identified in 36 (24.8%) out of 145 trichostrongylid egg positive... (More)

This study was conducted to determine the molecular prevalence and characterization of Haemonchus contortus from sheep along with the benzimidazole (BZ) resistance in H. contortus populations. Fecal samples were collected from a total of 300 sheep in research area and analyzed by fecal flotation. qPCR assays were utilized on trichostrongylid egg positive samples in order to identify H. contortus. Phylogenetic analyses were performed on ribosomal ITS-2 and mt-COI gene regions. BZ sensitive and resistant allele frequencies were determined by qPCR along with sequence analyses of the ß-tubulin isotype 1 gene for single nucleotide polimorphizms (SNPs). H. contortus was identified in 36 (24.8%) out of 145 trichostrongylid egg positive samples. H. contortus isolates showed 100.0% identity to each other and 0.1% difference with the isolates available in the GenBank based on phylogenetic analyses of ITS-2 gene while mt-COI analyses of the isolates exhibited a mean of 97.4±0.5% identity to each other and 5.5±0.8% difference with the isolates in GenBank. BZ sensitive and resistant allele frequencies in H. contortus populations were determined as 87.1%±16.2 and 12.9%±16.2, respectively. SNPs were detected only in the codon 200 of the sequenced isolates belong to resistant allele. This study provides the first data on molecular prevalence, phylogenetic characterization and BZ resistance in H. contortus populations from sheep in Turkey.

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author
alternative title
Molecular prevalence, phylogenetic characterization and benzimidazole resistance of Haemonchus contortus from sheep
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Benzimidazole resistance, Haemonchus contortus, Molecular characterization, Sheep
in
Kafkas Universitesi Veteriner Fakultesi Dergisi
volume
22
issue
1
pages
7 pages
publisher
Kafkas University
external identifiers
  • scopus:84946615721
ISSN
1300-6045
DOI
10.9775/kvfd.2015.13960
language
Turkish
LU publication?
no
id
245f768a-29aa-41ed-9af4-71b43035be56
date added to LUP
2017-12-04 15:14:02
date last changed
2019-06-19 03:43:53
@article{245f768a-29aa-41ed-9af4-71b43035be56,
  abstract     = {<p>This study was conducted to determine the molecular prevalence and characterization of Haemonchus contortus from sheep along with the benzimidazole (BZ) resistance in H. contortus populations. Fecal samples were collected from a total of 300 sheep in research area and analyzed by fecal flotation. qPCR assays were utilized on trichostrongylid egg positive samples in order to identify H. contortus. Phylogenetic analyses were performed on ribosomal ITS-2 and mt-COI gene regions. BZ sensitive and resistant allele frequencies were determined by qPCR along with sequence analyses of the ß-tubulin isotype 1 gene for single nucleotide polimorphizms (SNPs). H. contortus was identified in 36 (24.8%) out of 145 trichostrongylid egg positive samples. H. contortus isolates showed 100.0% identity to each other and 0.1% difference with the isolates available in the GenBank based on phylogenetic analyses of ITS-2 gene while mt-COI analyses of the isolates exhibited a mean of 97.4±0.5% identity to each other and 5.5±0.8% difference with the isolates in GenBank. BZ sensitive and resistant allele frequencies in H. contortus populations were determined as 87.1%±16.2 and 12.9%±16.2, respectively. SNPs were detected only in the codon 200 of the sequenced isolates belong to resistant allele. This study provides the first data on molecular prevalence, phylogenetic characterization and BZ resistance in H. contortus populations from sheep in Turkey.</p>},
  author       = {Önder, Zuhal and Yildirim, Alparslan and İnci, Abdullah and Düzlü, Önder and Çiloğlu, Arif},
  issn         = {1300-6045},
  keyword      = {Benzimidazole resistance,Haemonchus contortus,Molecular characterization,Sheep},
  language     = {tur},
  month        = {01},
  number       = {1},
  pages        = {93--99},
  publisher    = {Kafkas University},
  series       = {Kafkas Universitesi Veteriner Fakultesi Dergisi},
  title        = {Koyunlarda Haemonchus contortus’un moleküler prevalansı, filogenetik karakterizasyonu ve benzimidazol dirençliliği},
  url          = {http://dx.doi.org/10.9775/kvfd.2015.13960},
  volume       = {22},
  year         = {2016},
}