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Detection of laminin 5-specific auto-antibodies in mucous membrane and bullous pemphigoid sera by ELISA

Bekou, V; Thoma-Uszynski, S; Wendler, O; Uter, W; Schwietzke, S; Hunziker, T; Zouboulis, C C; Schuler, G; Sorokin, Lydia LU and Hertl, M (2005) In Journal of Investigative Dermatology 124(4). p.732-740
Abstract
Mucous membrane pemphigoid (MMP) is an autoimmune bullous disease that primarily affects mucous membranes leading to a scarring phenotype. MMP patients produce auto-antibodies (auto-ab) that preferentially recognize two components of the dermoepidermal basement membrane zone (BMZ): bullous pemphigoid (BP)180 and laminin 5 (LN5). Since detection of disease-specific auto-ab may be critical for diagnosis of MMP, we developed an ELISA with affinity-purified native human LN5. A total of 24 MMP, 72 BP, and 51 control sera were analyzed for LN5-specific auto-ab: 18/24 (75.0%) MMP and 29/72 (40.3%) BP sera were LN5 reactive. Sensitivity and specificity of the LN5 ELISA for MMP were 75% and 84.3%, respectively, and 40.3% and 88.2% for BP,... (More)
Mucous membrane pemphigoid (MMP) is an autoimmune bullous disease that primarily affects mucous membranes leading to a scarring phenotype. MMP patients produce auto-antibodies (auto-ab) that preferentially recognize two components of the dermoepidermal basement membrane zone (BMZ): bullous pemphigoid (BP)180 and laminin 5 (LN5). Since detection of disease-specific auto-ab may be critical for diagnosis of MMP, we developed an ELISA with affinity-purified native human LN5. A total of 24 MMP, 72 BP, and 51 control sera were analyzed for LN5-specific auto-ab: 18/24 (75.0%) MMP and 29/72 (40.3%) BP sera were LN5 reactive. Sensitivity and specificity of the LN5 ELISA for MMP were 75% and 84.3%, respectively, and 40.3% and 88.2% for BP, respectively. The LN5 ELISA was more sensitive than a dot blot assay with native LN5, which detected LN5-reactive IgG in 14/24 (58.3%) MMP and 16/72 (22.2%) BP sera. In MMP, but not BP, levels of LN5-reactive IgG correlated with disease severity. Furthermore, IgG reactivity to LN5 of the MMP and BP sera was not significantly associated with IgG reactivity against other autoantigens of the BMZ, such as BP180 or BP230. Thus, the established LN5 ELISA holds great promise as a novel diagnostic and prognostic parameter for MMP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BP180, dermoepidermal junction-auto-antibody, laminin 5, pemphigoid
in
Journal of Investigative Dermatology
volume
124
issue
4
pages
732 - 740
publisher
Nature Publishing Group
external identifiers
  • wos:000227776700011
  • pmid:15816831
  • scopus:20144386869
ISSN
1523-1747
DOI
10.1111/j.0022-202X.2005.23646.x
language
English
LU publication?
yes
id
637968ad-53c8-4130-965a-7eee234a9c74 (old id 247939)
date added to LUP
2007-08-08 13:43:55
date last changed
2017-08-06 03:38:10
@article{637968ad-53c8-4130-965a-7eee234a9c74,
  abstract     = {Mucous membrane pemphigoid (MMP) is an autoimmune bullous disease that primarily affects mucous membranes leading to a scarring phenotype. MMP patients produce auto-antibodies (auto-ab) that preferentially recognize two components of the dermoepidermal basement membrane zone (BMZ): bullous pemphigoid (BP)180 and laminin 5 (LN5). Since detection of disease-specific auto-ab may be critical for diagnosis of MMP, we developed an ELISA with affinity-purified native human LN5. A total of 24 MMP, 72 BP, and 51 control sera were analyzed for LN5-specific auto-ab: 18/24 (75.0%) MMP and 29/72 (40.3%) BP sera were LN5 reactive. Sensitivity and specificity of the LN5 ELISA for MMP were 75% and 84.3%, respectively, and 40.3% and 88.2% for BP, respectively. The LN5 ELISA was more sensitive than a dot blot assay with native LN5, which detected LN5-reactive IgG in 14/24 (58.3%) MMP and 16/72 (22.2%) BP sera. In MMP, but not BP, levels of LN5-reactive IgG correlated with disease severity. Furthermore, IgG reactivity to LN5 of the MMP and BP sera was not significantly associated with IgG reactivity against other autoantigens of the BMZ, such as BP180 or BP230. Thus, the established LN5 ELISA holds great promise as a novel diagnostic and prognostic parameter for MMP.},
  author       = {Bekou, V and Thoma-Uszynski, S and Wendler, O and Uter, W and Schwietzke, S and Hunziker, T and Zouboulis, C C and Schuler, G and Sorokin, Lydia and Hertl, M},
  issn         = {1523-1747},
  keyword      = {BP180,dermoepidermal junction-auto-antibody,laminin 5,pemphigoid},
  language     = {eng},
  number       = {4},
  pages        = {732--740},
  publisher    = {Nature Publishing Group},
  series       = {Journal of Investigative Dermatology},
  title        = {Detection of laminin 5-specific auto-antibodies in mucous membrane and bullous pemphigoid sera by ELISA},
  url          = {http://dx.doi.org/10.1111/j.0022-202X.2005.23646.x},
  volume       = {124},
  year         = {2005},
}